ID REPJ_ECOLX Reviewed; 279 AA.
AC P20356;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 27-MAR-2024, entry version 95.
DE RecName: Full=Regulatory protein RepA;
GN Name=repA;
OS Escherichia coli.
OG Plasmid IncQ RSF1010.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2653965; DOI=10.1016/0378-1119(89)90273-4;
RA Scholz P., Haring V., Wittmann-Liebold B., Ashman K., Bagdasarian M.,
RA Scherzinger E.;
RT "Complete nucleotide sequence and gene organization of the broad-host-range
RT plasmid RSF1010.";
RL Gene 75:271-288(1989).
CC -!- FUNCTION: This protein is involved in regulating the plasmid copy-
CC number. Increasing the level of this protein results in a higher
CC plasmid copy-number.
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DR EMBL; M28829; AAA26450.1; -; Genomic_DNA.
DR PIR; JH0130; RGECRI.
DR RefSeq; NP_044309.1; NC_001740.1.
DR RefSeq; WP_000190708.1; NZ_WAJL01000044.1.
DR PDB; 1G8Y; X-ray; 2.40 A; A/B/C/D/E/F/G/H/I/J/K/L=1-279.
DR PDB; 1NLF; X-ray; 1.95 A; A/B/C=1-279.
DR PDB; 1OLO; X-ray; 2.55 A; A/B=1-279.
DR PDBsum; 1G8Y; -.
DR PDBsum; 1NLF; -.
DR PDBsum; 1OLO; -.
DR AlphaFoldDB; P20356; -.
DR SMR; P20356; -.
DR DIP; DIP-59501N; -.
DR IntAct; P20356; 1.
DR GeneID; 84568267; -.
DR BRENDA; 3.6.4.12; 2026.
DR EvolutionaryTrace; P20356; -.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0006276; P:plasmid maintenance; IEA:UniProtKB-KW.
DR CDD; cd01125; RepA_RSF1010_like; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR038724; RepA.
DR Pfam; PF13481; AAA_25; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Nucleotide-binding; Plasmid;
KW Plasmid copy control.
FT CHAIN 1..279
FT /note="Regulatory protein RepA"
FT /id="PRO_0000068333"
FT HELIX 9..14
FT /evidence="ECO:0007829|PDB:1NLF"
FT STRAND 22..24
FT /evidence="ECO:0007829|PDB:1NLF"
FT STRAND 31..38
FT /evidence="ECO:0007829|PDB:1NLF"
FT HELIX 43..55
FT /evidence="ECO:0007829|PDB:1NLF"
FT STRAND 71..78
FT /evidence="ECO:0007829|PDB:1NLF"
FT HELIX 80..91
FT /evidence="ECO:0007829|PDB:1NLF"
FT HELIX 96..105
FT /evidence="ECO:0007829|PDB:1NLF"
FT STRAND 106..108
FT /evidence="ECO:0007829|PDB:1NLF"
FT TURN 112..114
FT /evidence="ECO:0007829|PDB:1OLO"
FT HELIX 121..131
FT /evidence="ECO:0007829|PDB:1NLF"
FT STRAND 135..140
FT /evidence="ECO:0007829|PDB:1NLF"
FT HELIX 142..145
FT /evidence="ECO:0007829|PDB:1NLF"
FT HELIX 153..170
FT /evidence="ECO:0007829|PDB:1NLF"
FT STRAND 173..179
FT /evidence="ECO:0007829|PDB:1NLF"
FT HELIX 203..205
FT /evidence="ECO:0007829|PDB:1NLF"
FT STRAND 209..214
FT /evidence="ECO:0007829|PDB:1NLF"
FT HELIX 217..222
FT /evidence="ECO:0007829|PDB:1NLF"
FT STRAND 227..229
FT /evidence="ECO:0007829|PDB:1G8Y"
FT HELIX 230..232
FT /evidence="ECO:0007829|PDB:1NLF"
FT STRAND 233..240
FT /evidence="ECO:0007829|PDB:1NLF"
FT STRAND 242..245
FT /evidence="ECO:0007829|PDB:1NLF"
FT STRAND 250..254
FT /evidence="ECO:0007829|PDB:1NLF"
FT HELIX 256..258
FT /evidence="ECO:0007829|PDB:1NLF"
FT STRAND 260..262
FT /evidence="ECO:0007829|PDB:1NLF"
SQ SEQUENCE 279 AA; 29909 MW; 69CD06FF8D3FFBB2 CRC64;
MATHKPINIL EAFAAAPPPL DYVLPNMVAG TVGALVSPGG AGKSMLALQL AAQIAGGPDL
LEVGELPTGP VIYLPAEDPP TAIHHRLHAL GAHLSAEERQ AVADGLLIQP LIGSLPNIMA
PEWFDGLKRA AEGRRLMVLD TLRRFHIEEE NASGPMAQVI GRMEAIAADT GCSIVFLHHA
SKGAAMMGAG DQQQASRGSS VLVDNIRWQS YLSSMTSAEA EEWGVDDDQR RFFVRFGVSK
ANYGAPFADR WFRRHDGGVL KPAVLERQRK SKGVPRGEA
//
