ID S5T4L2_9GAMM Unreviewed; 423 AA.
AC S5T4L2;
DT 16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT 16-OCT-2013, sequence version 1.
DT 24-JAN-2024, entry version 42.
DE RecName: Full=3-deoxy-D-manno-octulosonic acid transferase {ECO:0000256|ARBA:ARBA00019077, ECO:0000256|RuleBase:RU365103};
DE Short=Kdo transferase {ECO:0000256|RuleBase:RU365103};
DE EC=2.4.99.12 {ECO:0000256|ARBA:ARBA00012621, ECO:0000256|RuleBase:RU365103};
DE AltName: Full=Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase {ECO:0000256|ARBA:ARBA00031445, ECO:0000256|RuleBase:RU365103};
GN ORFNames=CYCME_0182 {ECO:0000313|EMBL:AGS38524.1};
OS Cycloclasticus zancles 78-ME.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Thiotrichales;
OC Piscirickettsiaceae; Cycloclasticus.
OX NCBI_TaxID=1198232 {ECO:0000313|EMBL:AGS38524.1, ECO:0000313|Proteomes:UP000015380};
RN [1] {ECO:0000313|EMBL:AGS38524.1, ECO:0000313|Proteomes:UP000015380}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=7-ME {ECO:0000313|Proteomes:UP000015380};
RA Yakimov M.M., Messina E., Genovese M., Denaro R., Crisafi F., Russo D.,
RA Cappello S., Santisi S., Smedile F., Golyshina O.V., Tran H., Pieper D.H.,
RA Golyshin P.N., Giuliano L.;
RT "Between feast and famine: a lifestyle of most important marine PAH-
RT degrading bacterium Cycloclasticus sp. 7ME.";
RL Submitted (MAY-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes
CC the transfer of 3-deoxy-D-manno-octulosonate (Kdo) residue(s) from CMP-
CC Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate
CC precursor of lipid A. {ECO:0000256|RuleBase:RU365103}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=CMP-3-deoxy-beta-D-manno-octulosonate + lipid IVA (E. coli) =
CC alpha-Kdo-(2->6)-lipid IVA (E. coli) + CMP + H(+);
CC Xref=Rhea:RHEA:28066, ChEBI:CHEBI:15378, ChEBI:CHEBI:58603,
CC ChEBI:CHEBI:60364, ChEBI:CHEBI:60377, ChEBI:CHEBI:85987;
CC EC=2.4.99.12; Evidence={ECO:0000256|ARBA:ARBA00034406,
CC ECO:0000256|RuleBase:RU365103};
CC -!- PATHWAY: Bacterial outer membrane biogenesis; LPS core biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004713, ECO:0000256|RuleBase:RU365103}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|RuleBase:RU365103}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC {ECO:0000256|RuleBase:RU365103}.
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DR EMBL; CP005996; AGS38524.1; -; Genomic_DNA.
DR RefSeq; WP_020931853.1; NC_021917.1.
DR AlphaFoldDB; S5T4L2; -.
DR KEGG; cza:CYCME_0182; -.
DR PATRIC; fig|1198232.3.peg.184; -.
DR eggNOG; COG1519; Bacteria.
DR HOGENOM; CLU_036146_2_0_6; -.
DR UniPathway; UPA00958; -.
DR Proteomes; UP000015380; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043842; F:Kdo transferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009244; P:lipopolysaccharide core region biosynthetic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.11720; 3-Deoxy-D-manno-octulosonic-acid transferase, N-terminal domain; 1.
DR Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 1.
DR InterPro; IPR007507; Glycos_transf_N.
DR InterPro; IPR038107; Glycos_transf_N_sf.
DR InterPro; IPR039901; Kdotransferase.
DR PANTHER; PTHR42755:SF1; 3-DEOXY-D-MANNO-OCTULOSONIC ACID TRANSFERASE, MITOCHONDRIAL-RELATED; 1.
DR PANTHER; PTHR42755; 3-DEOXY-MANNO-OCTULOSONATE CYTIDYLYLTRANSFERASE; 1.
DR Pfam; PF04413; Glycos_transf_N; 1.
DR SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|RuleBase:RU365103};
KW Glycosyltransferase {ECO:0000313|EMBL:AGS38524.1};
KW Lipopolysaccharide biosynthesis {ECO:0000256|RuleBase:RU365103};
KW Membrane {ECO:0000256|RuleBase:RU365103};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU365103}.
FT DOMAIN 33..209
FT /note="3-deoxy-D-manno-octulosonic-acid transferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF04413"
FT ACT_SITE 59
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR639901-1"
FT SITE 129
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR639901-2"
FT SITE 207
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR639901-2"
SQ SEQUENCE 423 AA; 47573 MW; 8481F8716A56355F CRC64;
MRLAYSVLLY LLTPFFILRL LIRGRKAPAY LNRWAERFGW YKKGQPQGVI WFHTVSVGEA
EAAFALIDKI AAAYPDKPIL VTTTTPTGSA RVKAFLGYRV HHVYLPYDLP DAMWRFYRCF
KPAIAIILET EIWPNMLHQA QKNAVPSIIV NARLSEKSAK GYAKLGAFMV QTLANITHVC
AQTEATLERF VDLGLNKNNI SVPGNIKFDL EMSAHLFEEA EVIRRDWFQQ RPSWIAASTH
EGEDEIVLDA FSQIKKQLPN SLLVLVPRHP ERFNTVAKLC EKRGYTVTRR SEQKSSPVKT
DVFLLDTLGE LKLYYATVDV AFIGGSFVPT GGHNMLEAAA HSVPVLFGPF VHNFTEISEC
LLKKGAAIQV MGADELALQV VDLLEHPEQR DQMGMKGLRF VEQNKGAVDK VAERIVSMVN
ASR
//
