ID SEM3B_HUMAN Reviewed; 749 AA.
AC Q13214; Q6GU46; Q8TB71; Q8TDV7; Q93018; Q96GX0;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 27-MAR-2024, entry version 195.
DE RecName: Full=Semaphorin-3B;
DE AltName: Full=Sema A(V);
DE AltName: Full=Semaphorin-V;
DE Short=Sema V;
DE Flags: Precursor;
GN Name=SEMA3B; Synonyms=SEMA5, SEMAA;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=8633026; DOI=10.1073/pnas.93.9.4120;
RA Sekido Y., Bader S., Latif F., Chen J.-Y., Duh F.-M., Wei M.-H.,
RA Albanesi J.P., Lee C.-C., Lerman M.I., Minna J.D.;
RT "Human semaphorins A(V) and IV reside in the 3p21.3 small cell lung cancer
RT deletion region and demonstrate distinct expression patterns.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:4120-4125(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Koyama N.;
RT "Semaphorin 3B (SEMA3B) cDNA.";
RL Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16641997; DOI=10.1038/nature04728;
RA Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J.,
RA Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P.,
RA Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A.,
RA Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G.,
RA Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W.,
RA Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M.,
RA Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P.,
RA Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H.,
RA Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J.,
RA Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W.,
RA Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B.,
RA Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O.,
RA Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B.,
RA Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H.,
RA Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J.,
RA Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X.,
RA Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R.,
RA Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.;
RT "The DNA sequence, annotation and analysis of human chromosome 3.";
RL Nature 440:1194-1198(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Brain, and Muscle;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP DISCUSSION OF SEQUENCE, AND VARIANTS CYS-348; HIS-397 AND ILE-415.
RX PubMed=11085536;
RG The international lung cancer chromosome 3p21.3 tumor suppressor gene consortium;
RA Lerman M.I., Minna J.D.;
RT "The 630-kb lung cancer homozygous deletion region on human chromosome
RT 3p21.3: identification and evaluation of the resident candidate tumor
RT suppressor genes.";
RL Cancer Res. 60:6116-6133(2000).
CC -!- FUNCTION: Inhibits axonal extension by providing local signals to
CC specify territories inaccessible for growing axons. {ECO:0000250}.
CC -!- INTERACTION:
CC Q13214-2; Q8N9W6-4: BOLL; NbExp=3; IntAct=EBI-11017428, EBI-11983447;
CC Q13214-2; Q9BWT7: CARD10; NbExp=3; IntAct=EBI-11017428, EBI-3866279;
CC Q13214-2; A8MQ03: CYSRT1; NbExp=3; IntAct=EBI-11017428, EBI-3867333;
CC Q13214-2; O75031: HSF2BP; NbExp=3; IntAct=EBI-11017428, EBI-7116203;
CC Q13214-2; Q5T749: KPRP; NbExp=3; IntAct=EBI-11017428, EBI-10981970;
CC Q13214-2; Q07627: KRTAP1-1; NbExp=3; IntAct=EBI-11017428, EBI-11959885;
CC Q13214-2; P60410: KRTAP10-8; NbExp=3; IntAct=EBI-11017428, EBI-10171774;
CC Q13214-2; P60411: KRTAP10-9; NbExp=3; IntAct=EBI-11017428, EBI-10172052;
CC Q13214-2; P59991: KRTAP12-2; NbExp=3; IntAct=EBI-11017428, EBI-10176379;
CC Q13214-2; Q3LI72: KRTAP19-5; NbExp=3; IntAct=EBI-11017428, EBI-1048945;
CC Q13214-2; P26371: KRTAP5-9; NbExp=3; IntAct=EBI-11017428, EBI-3958099;
CC Q13214-2; Q99750: MDFI; NbExp=3; IntAct=EBI-11017428, EBI-724076;
CC Q13214-2; P41227: NAA10; NbExp=3; IntAct=EBI-11017428, EBI-747693;
CC Q13214-2; P0DPK4: NOTCH2NLC; NbExp=3; IntAct=EBI-11017428, EBI-22310682;
CC Q13214-2; Q9NRD5: PICK1; NbExp=3; IntAct=EBI-11017428, EBI-79165;
CC Q13214-2; Q6ZRY4: RBPMS2; NbExp=3; IntAct=EBI-11017428, EBI-11987469;
CC Q13214-2; Q15645: TRIP13; NbExp=3; IntAct=EBI-11017428, EBI-358993;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}. Endoplasmic reticulum.
CC Note=Accumulates in the endoplasmic reticulum.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q13214-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q13214-2; Sequence=VSP_023032;
CC -!- TISSUE SPECIFICITY: Expressed abundantly but differentially in a
CC variety of neural and nonneural tissues.
CC -!- SIMILARITY: Belongs to the semaphorin family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC02731.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and
CC Haematology;
CC URL="https://atlasgeneticsoncology.org/gene/42252/SEMA3B";
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DR EMBL; U28369; AAD09138.1; -; mRNA.
DR EMBL; AB083186; BAB88870.1; -; mRNA.
DR EMBL; U73167; AAC02731.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BC009113; AAH09113.1; -; mRNA.
DR EMBL; BC013975; AAH13975.1; -; mRNA.
DR EMBL; BC024220; AAH24220.1; -; mRNA.
DR CCDS; CCDS74941.1; -. [Q13214-1]
DR CCDS; CCDS77744.1; -. [Q13214-2]
DR PIR; G01856; G01856.
DR RefSeq; NP_001005914.1; NM_001005914.2. [Q13214-2]
DR RefSeq; NP_001276989.1; NM_001290060.1. [Q13214-1]
DR RefSeq; NP_001276990.1; NM_001290061.1.
DR RefSeq; NP_001276991.1; NM_001290062.1.
DR RefSeq; NP_001276992.1; NM_001290063.1.
DR RefSeq; NP_004627.1; NM_004636.3. [Q13214-1]
DR AlphaFoldDB; Q13214; -.
DR SMR; Q13214; -.
DR BioGRID; 113618; 124.
DR IntAct; Q13214; 23.
DR STRING; 9606.ENSP00000480680; -.
DR GlyCosmos; Q13214; 3 sites, No reported glycans.
DR GlyGen; Q13214; 5 sites, 1 O-linked glycan (2 sites).
DR iPTMnet; Q13214; -.
DR PhosphoSitePlus; Q13214; -.
DR BioMuta; SEMA3B; -.
DR DMDM; 8134673; -.
DR EPD; Q13214; -.
DR jPOST; Q13214; -.
DR MassIVE; Q13214; -.
DR MaxQB; Q13214; -.
DR PaxDb; 9606-ENSP00000484146; -.
DR PeptideAtlas; Q13214; -.
DR ProteomicsDB; 59226; -. [Q13214-1]
DR ProteomicsDB; 59227; -. [Q13214-2]
DR Pumba; Q13214; -.
DR Antibodypedia; 30821; 158 antibodies from 20 providers.
DR DNASU; 7869; -.
DR Ensembl; ENST00000433753.4; ENSP00000485281.1; ENSG00000012171.20. [Q13214-2]
DR Ensembl; ENST00000616701.5; ENSP00000484146.1; ENSG00000012171.20. [Q13214-1]
DR Ensembl; ENST00000618865.4; ENSP00000481957.1; ENSG00000012171.20. [Q13214-1]
DR GeneID; 7869; -.
DR KEGG; hsa:7869; -.
DR MANE-Select; ENST00000616701.5; ENSP00000484146.1; NM_001290060.2; NP_001276989.1.
DR UCSC; uc032rmz.2; human. [Q13214-1]
DR AGR; HGNC:10724; -.
DR CTD; 7869; -.
DR DisGeNET; 7869; -.
DR GeneCards; SEMA3B; -.
DR HGNC; HGNC:10724; SEMA3B.
DR HPA; ENSG00000012171; Tissue enhanced (adrenal gland, brain).
DR MIM; 601281; gene.
DR neXtProt; NX_Q13214; -.
DR OpenTargets; ENSG00000012171; -.
DR PharmGKB; PA35646; -.
DR VEuPathDB; HostDB:ENSG00000012171; -.
DR eggNOG; KOG3611; Eukaryota.
DR GeneTree; ENSGT00940000157756; -.
DR HOGENOM; CLU_009051_5_1_1; -.
DR InParanoid; Q13214; -.
DR OrthoDB; 5342713at2759; -.
DR PhylomeDB; Q13214; -.
DR PathwayCommons; Q13214; -.
DR SignaLink; Q13214; -.
DR SIGNOR; Q13214; -.
DR BioGRID-ORCS; 7869; 3 hits in 266 CRISPR screens.
DR ChiTaRS; SEMA3B; human.
DR GeneWiki; SEMA3B; -.
DR GenomeRNAi; 7869; -.
DR Pharos; Q13214; Tbio.
DR PRO; PR:Q13214; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; Q13214; Protein.
DR Bgee; ENSG00000012171; Expressed in tibial nerve and 181 other cell types or tissues.
DR ExpressionAtlas; Q13214; baseline and differential.
DR Genevisible; Q13214; HS.
DR GO; GO:0062023; C:collagen-containing extracellular matrix; HDA:BHF-UCL.
DR GO; GO:0005783; C:endoplasmic reticulum; TAS:ProtInc.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0045499; F:chemorepellent activity; IBA:GO_Central.
DR GO; GO:0030215; F:semaphorin receptor binding; IBA:GO_Central.
DR GO; GO:0007411; P:axon guidance; IBA:GO_Central.
DR GO; GO:0007267; P:cell-cell signaling; TAS:ProtInc.
DR GO; GO:0061643; P:chemorepulsion of axon; TAS:ARUK-UCL.
DR GO; GO:0050919; P:negative chemotaxis; IBA:GO_Central.
DR GO; GO:0048843; P:negative regulation of axon extension involved in axon guidance; IBA:GO_Central.
DR GO; GO:0001755; P:neural crest cell migration; IBA:GO_Central.
DR GO; GO:0030335; P:positive regulation of cell migration; IBA:GO_Central.
DR GO; GO:0071526; P:semaphorin-plexin signaling pathway; IBA:GO_Central.
DR CDD; cd11250; Sema_3B; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR Gene3D; 3.30.1680.10; ligand-binding face of the semaphorins, domain 2; 1.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR013151; Immunoglobulin.
DR InterPro; IPR016201; PSI.
DR InterPro; IPR001627; Semap_dom.
DR InterPro; IPR036352; Semap_dom_sf.
DR InterPro; IPR027231; Semaphorin.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR PANTHER; PTHR11036; SEMAPHORIN; 1.
DR PANTHER; PTHR11036:SF37; SEMAPHORIN-3B; 1.
DR Pfam; PF00047; ig; 1.
DR Pfam; PF01403; Sema; 1.
DR SMART; SM00409; IG; 1.
DR SMART; SM00423; PSI; 1.
DR SMART; SM00630; Sema; 1.
DR SUPFAM; SSF48726; Immunoglobulin; 1.
DR SUPFAM; SSF103575; Plexin repeat; 1.
DR SUPFAM; SSF101912; Sema domain; 1.
DR PROSITE; PS50835; IG_LIKE; 1.
DR PROSITE; PS51004; SEMA; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Disulfide bond; Endoplasmic reticulum; Glycoprotein;
KW Immunoglobulin domain; Reference proteome; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..749
FT /note="Semaphorin-3B"
FT /id="PRO_0000032309"
FT DOMAIN 30..513
FT /note="Sema"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00352"
FT DOMAIN 573..659
FT /note="Ig-like C2-type"
FT REGION 702..749
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 733..749
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 82
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 124
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 427
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 102..113
FT /evidence="ECO:0000250"
FT DISULFID 131..140
FT /evidence="ECO:0000250"
FT DISULFID 269..380
FT /evidence="ECO:0000250"
FT DISULFID 293..340
FT /evidence="ECO:0000250"
FT DISULFID 516..534
FT /evidence="ECO:0000250"
FT DISULFID 644..710
FT /evidence="ECO:0000250"
FT VAR_SEQ 332
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023032"
FT VARIANT 348
FT /note="R -> C (in a non-small cell lung cancer cell line)"
FT /evidence="ECO:0000269|PubMed:11085536"
FT /id="VAR_014221"
FT VARIANT 397
FT /note="D -> H (in a non-small cell lung cancer cell line)"
FT /evidence="ECO:0000269|PubMed:11085536"
FT /id="VAR_014222"
FT VARIANT 415
FT /note="T -> I (in a non-small cell lung cancer cell line;
FT dbSNP:rs2071203)"
FT /evidence="ECO:0000269|PubMed:11085536"
FT /id="VAR_014223"
FT CONFLICT 29..36
FT /note="PRLRLSFQ -> HAFGSPSKV (in Ref. 3; AAC02731)"
FT /evidence="ECO:0000305"
FT CONFLICT 308
FT /note="Q -> RPFPAE (in Ref. 2; BAB88870)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 749 AA; 83122 MW; 1F3B8F63F59444F3 CRC64;
MGRAGAAAVI PGLALLWAVG LGSAAPSPPR LRLSFQELQA WHGLQTFSLE RTCCYQALLV
DEERGRLFVG AENHVASLNL DNISKRAKKL AWPAPVEWRE ECNWAGKDIG TECMNFVKLL
HAYNRTHLLA CGTGAFHPTC AFVEVGHRAE EPVLRLDPGR IEDGKGKSPY DPRHRAASVL
VGEELYSGVA ADLMGRDFTI FRSLGQRPSL RTEPHDSRWL NEPKFVKVFW IPESENPDDD
KIYFFFRETA VEAAPALGRL SVSRVGQICR NDVGGQRSLV NKWTTFLKAR LVCSVPGVEG
DTHFDQLQDV FLLSSRDHRT PLLYAVFSTS SSIFQGSAVC VYSMNDVRRA FLGPFAHKEG
PMHQWVSYQG RVPYPRPGMC PSKTFGTFSS TKDFPDDVIQ FARNHPLMYN SVLPTGGRPL
FLQVGANYTF TQIAADRVAA ADGHYDVLFI GTDVGTVLKV ISVPKGSRPS AEGLLLEELH
VFEDSAAVTS MQISSKRHQL YVASRSAVAQ IALHRCAAHG RVCTECCLAR DPYCAWDGVA
CTRFQPSAKR RFRRQDVRNG DPSTLCSGDS SRPALLEHKV FGVEGSSAFL ECEPRSLQAR
VEWTFQRAGV TAHTQVLAEE RTERTARGLL LRRLRRRDSG VYLCAAVEQG FTQPLRRLSL
HVLSATQAER LARAEEAAPA APPGPKLWYR DFLQLVEPGG GGSANSLRMC RPQPALQSLP
LESRRKGRNR RTHAPEPRAE RGPRSATHW
//
