ID SMU1_DANRE Reviewed; 513 AA.
AC Q7ZVA0;
DT 30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 27-MAR-2024, entry version 113.
DE RecName: Full=WD40 repeat-containing protein SMU1;
DE AltName: Full=Smu-1 suppressor of mec-8 and unc-52 protein homolog;
GN Name=smu1; ORFNames=zgc:56147;
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=SJD;
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in pre-mRNA splicing as a component of the
CC spliceosome (By similarity). Required for normal accumulation of ik (By
CC similarity). Required for normal mitotic spindle assembly and normal
CC progress through mitosis (By similarity).
CC {ECO:0000250|UniProtKB:Q2TAY7, ECO:0000250|UniProtKB:Q76B40}.
CC -!- SUBUNIT: Component of the spliceosome B complex. Interacts with ik.
CC {ECO:0000250|UniProtKB:Q2TAY7}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q99M63}. Nucleus
CC {ECO:0000250|UniProtKB:Q76B40}. Nucleus speckle
CC {ECO:0000250|UniProtKB:Q76B40}. Note=Colocalizes with srsf1 in nuclear
CC speckles. {ECO:0000250|UniProtKB:Q76B40}.
CC -!- DOMAIN: The WD repeats assemble into a seven-bladed WD propeller.
CC {ECO:0000250|UniProtKB:G5EEG7}.
CC -!- SIMILARITY: Belongs to the WD repeat SMU1 family. {ECO:0000305}.
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DR EMBL; BC045945; AAH45945.1; -; mRNA.
DR RefSeq; NP_956493.1; NM_200199.1.
DR AlphaFoldDB; Q7ZVA0; -.
DR SMR; Q7ZVA0; -.
DR STRING; 7955.ENSDARP00000089117; -.
DR PaxDb; 7955-ENSDARP00000089117; -.
DR GeneID; 393168; -.
DR KEGG; dre:393168; -.
DR AGR; ZFIN:ZDB-GENE-040426-916; -.
DR CTD; 393168; -.
DR ZFIN; ZDB-GENE-040426-916; smu1a.
DR eggNOG; KOG0275; Eukaryota.
DR InParanoid; Q7ZVA0; -.
DR OrthoDB; 3893433at2759; -.
DR PhylomeDB; Q7ZVA0; -.
DR PRO; PR:Q7ZVA0; -.
DR Proteomes; UP000000437; Chromosome 14.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016607; C:nuclear speck; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0071011; C:precatalytic spliceosome; IBA:GO_Central.
DR GO; GO:0071005; C:U2-type precatalytic spliceosome; ISS:UniProtKB.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; ISS:UniProtKB.
DR GO; GO:0008380; P:RNA splicing; IBA:GO_Central.
DR CDD; cd00200; WD40; 1.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR InterPro; IPR006595; CTLH_C.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR006594; LisH.
DR InterPro; IPR045184; SMU1.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR PANTHER; PTHR22848; WD40 REPEAT PROTEIN; 1.
DR PANTHER; PTHR22848:SF0; WD40 REPEAT-CONTAINING PROTEIN SMU1; 1.
DR Pfam; PF17814; LisH_TPL; 1.
DR Pfam; PF00400; WD40; 5.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00668; CTLH; 1.
DR SMART; SM00667; LisH; 1.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
DR PROSITE; PS50897; CTLH; 1.
DR PROSITE; PS50896; LISH; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Repeat; Spliceosome; WD repeat.
FT CHAIN 1..513
FT /note="WD40 repeat-containing protein SMU1"
FT /id="PRO_0000237594"
FT DOMAIN 6..38
FT /note="LisH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00126"
FT DOMAIN 40..92
FT /note="CTLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00058"
FT REPEAT 212..253
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 262..303
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 305..346
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 347..386
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 395..436
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 440..479
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 482..513
FT /note="WD 7"
FT /evidence="ECO:0000255"
FT REGION 1..315
FT /note="Required for interaction with ik"
FT /evidence="ECO:0000250|UniProtKB:Q2TAY7"
SQ SEQUENCE 513 AA; 57531 MW; A0E11AA1760F3F4B CRC64;
MSIEIESADV IRLIMQYLKE NSLHRTLAML QEETTVSLNT VDSIESFVAD INSGHWDTVL
QAIQSLKLPD KTLIDLYEQV VLELIELREL GAARSLLRQT DPMIMLKQTQ PERYIHLENL
LARSYFDPRE AYPDGSSKEK RRAAIAQALA GEVSVVPPSR LMALLGQSLK WQQHQGLLPP
GMTIDLFRGK AAVKDVEEER FPTQLARHIK FGQKSHVECA RFSPDGQYLV TGSVDGFIEV
WNFTTGKIRK DLKYQAQDNF MMMDDAVLCM SFSRDTEMLA TGAQDGKIKV WKIQSGQCLR
RYERAHSKGV TCLSFSKDST QILSASFDQT IRIHGLKSGK CLKEFRGHSS FVNEATLTPD
GHHAISASSD GTVKVWNMKT TECTSTFKSL GTSAGTDITV NNVILLPKNP EHFVVCNRSN
TVVIMNMQGQ IVRSFSSGKR EGGDFVCCTL SPRGEWIYCV GEDYVLYCFS TVTGKLERTL
TVHEKDVIGI AHHPHQNLIG TYSEDGLLKL WKP
//
