ID SP17_HUMAN Reviewed; 151 AA.
AC Q15506; B2R4F2; Q9BXF7;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 24-JAN-2024, entry version 177.
DE RecName: Full=Sperm surface protein Sp17;
DE AltName: Full=Cancer/testis antigen 22;
DE Short=CT22;
DE AltName: Full=Sp17-1;
DE AltName: Full=Sperm autoantigenic protein 17;
DE Short=Sperm protein 17;
GN Name=SPA17; Synonyms=SP17;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RX PubMed=8688458; DOI=10.1016/0167-4781(96)00077-2;
RA Lea I.A., Richardson R.T., Widgren E.E., O'Rand M.G.;
RT "Cloning and sequencing of cDNAs encoding the human sperm protein, Sp17.";
RL Biochim. Biophys. Acta 1307:263-266(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Testis;
RX PubMed=12393185; DOI=10.1016/s0167-4781(02)00478-5;
RA Buchli R., De Jong A., Robbins D.L.;
RT "Genomic organization of an intron-containing sperm protein 17 gene (Sp17-
RT 1) and an intronless pseudogene (Sp17-2) in humans: a new model.";
RL Biochim. Biophys. Acta 1578:29-42(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP POSSIBLE INTERACTION WITH ROPN1.
RX PubMed=17551920; DOI=10.1002/ijc.22842;
RA Li Z., Li W., Meklat F., Wang Z., Zhang J., Zhang Y., Lim S.H.;
RT "A yeast two-hybrid system using Sp17 identified ropporin as a novel
RT cancer-testis antigen in hematologic malignancies.";
RL Int. J. Cancer 121:1507-1511(2007).
CC -!- FUNCTION: Sperm surface zona pellucida binding protein. Helps to bind
CC spermatozoa to the zona pellucida with high affinity. Might function in
CC binding zona pellucida and carbohydrates (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Homodimer (By similarity). May interact with ROPN1.
CC {ECO:0000250}.
CC -!- INTERACTION:
CC Q15506; P35609: ACTN2; NbExp=3; IntAct=EBI-1377865, EBI-77797;
CC Q15506; O43687-2: AKAP7; NbExp=6; IntAct=EBI-1377865, EBI-10185182;
CC Q15506; P14649: MYL6B; NbExp=12; IntAct=EBI-1377865, EBI-358570;
CC Q15506; Q9HAT0: ROPN1; NbExp=9; IntAct=EBI-1377865, EBI-1378139;
CC Q15506; Q96C74: ROPN1L; NbExp=4; IntAct=EBI-1377865, EBI-9033237;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Peripheral membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Testis and sperm specific.
CC -!- WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and
CC Haematology;
CC URL="https://atlasgeneticsoncology.org/gene/42360/SPA17";
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DR EMBL; Z48570; CAA88459.1; -; mRNA.
DR EMBL; AF334735; AAK20878.1; -; mRNA.
DR EMBL; AF334810; AAK28125.1; -; Genomic_DNA.
DR EMBL; AK311806; BAG34749.1; -; mRNA.
DR EMBL; CH471065; EAW67592.1; -; Genomic_DNA.
DR EMBL; BC032457; AAH32457.1; -; mRNA.
DR CCDS; CCDS8450.1; -.
DR PIR; I38243; I38243.
DR RefSeq; NP_059121.1; NM_017425.3.
DR RefSeq; XP_011541172.1; XM_011542870.2.
DR PDB; 8J07; EM; 4.10 A; K/L/k/l=1-151.
DR PDBsum; 8J07; -.
DR AlphaFoldDB; Q15506; -.
DR EMDB; EMD-35888; -.
DR SMR; Q15506; -.
DR BioGRID; 119741; 17.
DR IntAct; Q15506; 11.
DR MINT; Q15506; -.
DR STRING; 9606.ENSP00000432305; -.
DR GlyGen; Q15506; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q15506; -.
DR PhosphoSitePlus; Q15506; -.
DR BioMuta; SPA17; -.
DR DMDM; 2833264; -.
DR EPD; Q15506; -.
DR jPOST; Q15506; -.
DR MassIVE; Q15506; -.
DR MaxQB; Q15506; -.
DR PaxDb; 9606-ENSP00000432305; -.
DR PeptideAtlas; Q15506; -.
DR ProteomicsDB; 60613; -.
DR Pumba; Q15506; -.
DR TopDownProteomics; Q15506; -.
DR Antibodypedia; 32898; 306 antibodies from 26 providers.
DR DNASU; 53340; -.
DR Ensembl; ENST00000227135.7; ENSP00000227135.2; ENSG00000064199.7.
DR Ensembl; ENST00000532692.1; ENSP00000432305.1; ENSG00000064199.7.
DR GeneID; 53340; -.
DR KEGG; hsa:53340; -.
DR MANE-Select; ENST00000227135.7; ENSP00000227135.2; NM_017425.4; NP_059121.1.
DR UCSC; uc001qap.4; human.
DR AGR; HGNC:11210; -.
DR CTD; 53340; -.
DR DisGeNET; 53340; -.
DR GeneCards; SPA17; -.
DR HGNC; HGNC:11210; SPA17.
DR HPA; ENSG00000064199; Tissue enriched (testis).
DR MIM; 608621; gene.
DR neXtProt; NX_Q15506; -.
DR OpenTargets; ENSG00000064199; -.
DR PharmGKB; PA36047; -.
DR VEuPathDB; HostDB:ENSG00000064199; -.
DR eggNOG; ENOG502S4R6; Eukaryota.
DR GeneTree; ENSGT00440000039164; -.
DR HOGENOM; CLU_115900_0_0_1; -.
DR InParanoid; Q15506; -.
DR OMA; NCESEKE; -.
DR OrthoDB; 5472182at2759; -.
DR PhylomeDB; Q15506; -.
DR TreeFam; TF332959; -.
DR PathwayCommons; Q15506; -.
DR SignaLink; Q15506; -.
DR BioGRID-ORCS; 53340; 12 hits in 1118 CRISPR screens.
DR ChiTaRS; SPA17; human.
DR GeneWiki; SPA17; -.
DR GenomeRNAi; 53340; -.
DR Pharos; Q15506; Tbio.
DR PRO; PR:Q15506; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q15506; Protein.
DR Bgee; ENSG00000064199; Expressed in bronchial epithelial cell and 167 other cell types or tissues.
DR ExpressionAtlas; Q15506; baseline and differential.
DR Genevisible; Q15506; HS.
DR GO; GO:0005929; C:cilium; IDA:BHF-UCL.
DR GO; GO:0005737; C:cytoplasm; IDA:BHF-UCL.
DR GO; GO:0009897; C:external side of plasma membrane; IEA:Ensembl.
DR GO; GO:0005576; C:extracellular region; IEA:GOC.
DR GO; GO:0031514; C:motile cilium; IDA:BHF-UCL.
DR GO; GO:0043005; C:neuron projection; IEA:Ensembl.
DR GO; GO:0035686; C:sperm fibrous sheath; IDA:GO_Central.
DR GO; GO:0097228; C:sperm principal piece; IDA:GO_Central.
DR GO; GO:0005516; F:calmodulin binding; IBA:GO_Central.
DR GO; GO:0007339; P:binding of sperm to zona pellucida; IEA:InterPro.
DR GO; GO:0003351; P:epithelial cilium movement involved in extracellular fluid movement; NAS:BHF-UCL.
DR GO; GO:0007338; P:single fertilization; TAS:ProtInc.
DR GO; GO:0007283; P:spermatogenesis; TAS:ProtInc.
DR CDD; cd12100; DD_CABYR_SP17; 1.
DR Gene3D; 1.20.5.190; -; 1.
DR Gene3D; 1.20.890.10; cAMP-dependent protein kinase regulatory subunit, dimerization-anchoring domain; 1.
DR InterPro; IPR003117; cAMP_dep_PK_reg_su_I/II_a/b.
DR InterPro; IPR047579; DD_CABYR_SP17.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR InterPro; IPR012105; Sp17.
DR PANTHER; PTHR10699; NEUROMODULIN; 1.
DR PANTHER; PTHR10699:SF16; SPERM SURFACE PROTEIN SP17; 1.
DR Pfam; PF00612; IQ; 1.
DR Pfam; PF02197; RIIa; 1.
DR PIRSF; PIRSF016533; Sp17; 1.
DR SMART; SM00015; IQ; 1.
DR SMART; SM00394; RIIa; 1.
DR SUPFAM; SSF47391; Dimerization-anchoring domain of cAMP-dependent PK regulatory subunit; 1.
DR PROSITE; PS50096; IQ; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Membrane; Reference proteome.
FT CHAIN 1..151
FT /note="Sperm surface protein Sp17"
FT /id="PRO_0000181340"
FT DOMAIN 114..143
FT /note="IQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT REGION 56..115
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 127..151
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 151 AA; 17406 MW; BC99EC310FA0E54A CRC64;
MSIPFSNTHY RIPQGFGNLL EGLTREILRE QPDNIPAFAA AYFESLLEKR EKTNFDPAEW
GSKVEDRFYN NHAFEEQEPP EKSDPKQEES QISGKEEETS VTILDSSEED KEKEEVAAVK
IQAAFRGHIA REEAKKMKTN SLQNEEKEEN K
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