ID SYM_RICPR Reviewed; 508 AA.
AC Q9ZCP0;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 27-MAR-2024, entry version 124.
DE RecName: Full=Methionine--tRNA ligase;
DE EC=6.1.1.10;
DE AltName: Full=Methionyl-tRNA synthetase;
DE Short=MetRS;
GN Name=metG; Synonyms=metS; OrderedLocusNames=RP683;
OS Rickettsia prowazekii (strain Madrid E).
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rickettsiales;
OC Rickettsiaceae; Rickettsieae; Rickettsia; typhus group.
OX NCBI_TaxID=272947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Madrid E;
RX PubMed=9823893; DOI=10.1038/24094;
RA Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T.,
RA Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H.,
RA Kurland C.G.;
RT "The genome sequence of Rickettsia prowazekii and the origin of
RT mitochondria.";
RL Nature 396:133-140(1998).
CC -!- FUNCTION: Is required not only for elongation of protein synthesis but
CC also for the initiation of all mRNA translation through initiator
CC tRNA(fMet) aminoacylation. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-
CC methionyl-tRNA(Met); Xref=Rhea:RHEA:13481, Rhea:RHEA-COMP:9667,
CC Rhea:RHEA-COMP:9698, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:57844, ChEBI:CHEBI:78442, ChEBI:CHEBI:78530,
CC ChEBI:CHEBI:456215; EC=6.1.1.10;
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC MetG type 2B subfamily. {ECO:0000305}.
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DR EMBL; AJ235272; CAA15120.1; -; Genomic_DNA.
DR PIR; F71674; F71674.
DR RefSeq; NP_221044.1; NC_000963.1.
DR RefSeq; WP_004596363.1; NC_000963.1.
DR AlphaFoldDB; Q9ZCP0; -.
DR SMR; Q9ZCP0; -.
DR STRING; 272947.gene:17555760; -.
DR EnsemblBacteria; CAA15120; CAA15120; CAA15120.
DR GeneID; 57569808; -.
DR KEGG; rpr:RP683; -.
DR PATRIC; fig|272947.5.peg.705; -.
DR eggNOG; COG0143; Bacteria.
DR HOGENOM; CLU_009710_9_2_5; -.
DR OrthoDB; 9810191at2; -.
DR Proteomes; UP000002480; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004825; F:methionine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006431; P:methionyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR CDD; cd07957; Anticodon_Ia_Met; 1.
DR CDD; cd00814; MetRS_core; 1.
DR Gene3D; 2.170.220.10; -; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_01228; Met_tRNA_synth_type2; 1.
DR InterPro; IPR041872; Anticodon_Met.
DR InterPro; IPR014758; Met-tRNA_synth.
DR InterPro; IPR023457; Met-tRNA_synth_2.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR033911; MetRS_core.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR NCBIfam; TIGR00398; metG; 1.
DR PANTHER; PTHR43326:SF1; METHIONINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR43326; METHIONYL-TRNA SYNTHETASE; 1.
DR Pfam; PF19303; Anticodon_3; 1.
DR Pfam; PF09334; tRNA-synt_1g; 2.
DR PRINTS; PR01041; TRNASYNTHMET.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..508
FT /note="Methionine--tRNA ligase"
FT /id="PRO_0000139238"
FT MOTIF 12..22
FT /note="'HIGH' region"
FT MOTIF 295..299
FT /note="'KMSKS' region"
FT BINDING 298
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
SQ SEQUENCE 508 AA; 58592 MW; C781AB4C8A674205 CRC64;
MKNTYYITTP IYYVNDVAHI GHAYTSVASD VIARFMRFCG KDVMFLTGTD EHGQKVEKAA
INQNIDPQTF TDKTSQNFRD LMVAMNISND DFIRTTENRH KKAVAVFWKK LLDNGAIYEG
FYEGWYSVRD EAFYDESEIN EDKLAPTGAP VEWVKEPSYF FNLAKWQDKL LEFYELNPDF
VRPISRRNEV ISFIKSGLKD LSISRTTFHW GIKVPNNRKH VIYVWLDALV NYISALGYPD
KQSNYAKFWP ANLQIVGKDI LRFHAVYWPA FLMAAEIPLP KAIMVHGWWT NEGQKISKSL
GNTIDPITLI DEFGVDQVRY FLMREVIFGA DANFARNNLI TRINSELSNK IGNLLHRIVS
FVYKNNDAKV PLIKSGVIDK IYELPILKTA IKFAQENILL MDKTEINKIL ENIINLAEDA
NIYITNEAPW NLKTTDPDKM LEVLYSLLEV LRYIAIMLQP FVPNSANKML DQLGVSKEER
LFKHLVRDHA LKAGSNILEP SIIFPKFN
//
