ID T0GC68_9SPHN Unreviewed; 429 AA.
AC T0GC68;
DT 16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT 16-OCT-2013, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE SubName: Full=SAM-dependent methlyltransferase {ECO:0000313|EMBL:EQB01321.1};
GN ORFNames=L286_16145 {ECO:0000313|EMBL:EQB01321.1};
OS Sphingobium sp. HDIP04.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingobium.
OX NCBI_TaxID=428994 {ECO:0000313|EMBL:EQB01321.1, ECO:0000313|Proteomes:UP000015528};
RN [1] {ECO:0000313|EMBL:EQB01321.1, ECO:0000313|Proteomes:UP000015528}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HDIP04 {ECO:0000313|EMBL:EQB01321.1,
RC ECO:0000313|Proteomes:UP000015528};
RX PubMed=24051321;
RA Mukherjee U., Kumar R., Mahato N.K., Khurana J.P., Lal R.;
RT "Draft Genome Sequence of Sphingobium sp. Strain HDIPO4, an Avid Degrader
RT of Hexachlorocyclohexane.";
RL Genome Announc. 1:e00749-e00713(2013).
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RsmB/NOP family. {ECO:0000256|PROSITE-ProRule:PRU01023}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EQB01321.1}.
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DR EMBL; ATDO01000111; EQB01321.1; -; Genomic_DNA.
DR RefSeq; WP_020819044.1; NZ_ATDO01000111.1.
DR AlphaFoldDB; T0GC68; -.
DR PATRIC; fig|428994.3.peg.3086; -.
DR Proteomes; UP000015528; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:InterPro.
DR GO; GO:0001510; P:RNA methylation; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 1.10.940.10; NusB-like; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR InterPro; IPR035926; NusB-like_sf.
DR InterPro; IPR006027; NusB_RsmB_TIM44.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR22807:SF61; NOL1_NOP2_SUN FAMILY PROTEIN _ ANTITERMINATION NUSB DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR22807; NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR Pfam; PF01189; Methyltr_RsmB-F; 1.
DR Pfam; PF01029; NusB; 1.
DR PRINTS; PR02008; RCMTFAMILY.
DR SUPFAM; SSF48013; NusB-like; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01023};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU01023};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01023};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01023}.
FT DOMAIN 149..426
FT /note="SAM-dependent MTase RsmB/NOP-type"
FT /evidence="ECO:0000259|PROSITE:PS51686"
FT ACT_SITE 359
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 243..249
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 264
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 290
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 306
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ SEQUENCE 429 AA; 45388 MW; 646BD34237611C1D CRC64;
MPRPSRPQDV PGLPTRRAAL RLLDAVLRRG DPLELALHGA AQGLAPADRA LVHAIAAETL
RHLPDLDALI DGATRQPLPY DAKARMVLRI ALIQVLALGT APHAAIATAL PLVDGGPRKL
VHGVFGTLTR ADPTLPSPPA LPPETAGRWA AQWGDAMPQA AAHAYAQRPP VDVSLRDPAR
TAHWAEALNG RSLAPGHVRL PEGAVIPDLP GFGEGAWWVQ DLAASTPARL LGAGEGRCVL
DLCAAPGGKT MQLAAAGWRV TAVDQSKKRL ERLSENLART GLSAAIVQDD LRAWRPEAPA
DAVLLDAPCT ATGIYRRHPD VLHRIGPRQI AEMAELQAEL LARAAAWLKP GGTLVYATCS
LEQAEGEDQV ARFLDARPDF GPLPAMPAEL PDGIAPTAQG WVRTLPDSLA AQGGTDGFFV
ARLIRSTKP
//
