ID T1FXE2_HELRO Unreviewed; 290 AA.
AC T1FXE2;
DT 16-OCT-2013, integrated into UniProtKB/TrEMBL.
DT 16-OCT-2013, sequence version 1.
DT 27-MAR-2024, entry version 52.
DE RecName: Full=NADP-dependent oxidoreductase domain-containing protein {ECO:0000259|Pfam:PF00248};
GN Name=20213490 {ECO:0000313|EnsemblMetazoa:HelroP63316};
GN ORFNames=HELRODRAFT_63316 {ECO:0000313|EMBL:ESO12199.1};
OS Helobdella robusta (Californian leech).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Annelida; Clitellata;
OC Hirudinea; Rhynchobdellida; Glossiphoniidae; Helobdella.
OX NCBI_TaxID=6412 {ECO:0000313|EnsemblMetazoa:HelroP63316, ECO:0000313|Proteomes:UP000015101};
RN [1] {ECO:0000313|Proteomes:UP000015101}
RP NUCLEOTIDE SEQUENCE.
RA Hellsten U., Grimwood J., Chapman J.A., Shapiro H., Aerts A., Otillar R.P.,
RA Terry A.Y., Boore J.L., Simakov O., Marletaz F., Cho S.-J.,
RA Edsinger-Gonzales E., Havlak P., Kuo D.-H., Larsson T., Lv J., Arendt D.,
RA Savage R., Osoegawa K., de Jong P., Lindberg D.R., Seaver E.C.,
RA Weisblat D.A., Putnam N.H., Grigoriev I.V., Rokhsar D.S.;
RL Submitted (DEC-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ESO12199.1, ECO:0000313|Proteomes:UP000015101}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=23254933; DOI=10.1038/nature11696;
RA Simakov O., Marletaz F., Cho S.J., Edsinger-Gonzales E., Havlak P.,
RA Hellsten U., Kuo D.H., Larsson T., Lv J., Arendt D., Savage R.,
RA Osoegawa K., de Jong P., Grimwood J., Chapman J.A., Shapiro H., Aerts A.,
RA Otillar R.P., Terry A.Y., Boore J.L., Grigoriev I.V., Lindberg D.R.,
RA Seaver E.C., Weisblat D.A., Putnam N.H., Rokhsar D.S.;
RT "Insights into bilaterian evolution from three spiralian genomes.";
RL Nature 493:526-531(2013).
RN [3] {ECO:0000313|EnsemblMetazoa:HelroP63316}
RP IDENTIFICATION.
RG EnsemblMetazoa;
RL Submitted (JUN-2015) to UniProtKB.
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DR EMBL; AMQM01000098; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; KB095811; ESO12199.1; -; Genomic_DNA.
DR RefSeq; XP_009008919.1; XM_009010671.1.
DR AlphaFoldDB; T1FXE2; -.
DR STRING; 6412.T1FXE2; -.
DR EnsemblMetazoa; HelroT63316; HelroP63316; HelroG63316.
DR GeneID; 20213490; -.
DR KEGG; hro:HELRODRAFT_63316; -.
DR CTD; 20213490; -.
DR eggNOG; KOG1577; Eukaryota.
DR HOGENOM; CLU_023205_0_1_1; -.
DR InParanoid; T1FXE2; -.
DR OMA; IYAPVCW; -.
DR OrthoDB; 5305445at2759; -.
DR Proteomes; UP000015101; Unassembled WGS sequence.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:InterPro.
DR GO; GO:0071704; P:organic substance metabolic process; IEA:UniProt.
DR GO; GO:0044281; P:small molecule metabolic process; IEA:UniProt.
DR CDD; cd19136; AKR_DrGR-like; 1.
DR Gene3D; 3.20.20.100; NADP-dependent oxidoreductase domain; 1.
DR InterPro; IPR020471; AKR.
DR InterPro; IPR018170; Aldo/ket_reductase_CS.
DR InterPro; IPR023210; NADP_OxRdtase_dom.
DR InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR PANTHER; PTHR43827; 2,5-DIKETO-D-GLUCONIC ACID REDUCTASE; 1.
DR PANTHER; PTHR43827:SF15; ALDO_KET_RED DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF00248; Aldo_ket_red; 1.
DR PIRSF; PIRSF000097; AKR; 1.
DR PRINTS; PR00069; ALDKETRDTASE.
DR SUPFAM; SSF51430; NAD(P)-linked oxidoreductase; 1.
DR PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000015101}.
FT DOMAIN 18..277
FT /note="NADP-dependent oxidoreductase"
FT /evidence="ECO:0000259|Pfam:PF00248"
FT ACT_SITE 51
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-1"
FT BINDING 113
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-2"
FT SITE 80
FT /note="Lowers pKa of active site Tyr"
FT /evidence="ECO:0000256|PIRSR:PIRSR000097-3"
SQ SEQUENCE 290 AA; 33417 MW; 553C6F79887A48F4 CRC64;
MKCFDQVLLN SGFTMPMISL GTFKLKGDDV FQIIDAALNY GYRSFDTAQV YKNEESIGNA
LKILLPKYNL NRKDIFLITK LGPKNHGNVR CRVFYLQSLR NLQTDYLDLF LIHWPAVQGC
KPSDEINIKL RNESWQEMEK LVIEGKINSV GVSNYTMRHL EELYKYCKIE PSVLQIEYHP
HLVQSKLLQF CQKNNLHLQA YSSLGSQGNN LLLSNNLVRQ IALKHAVTNA QVLLKWAAQQ
NIGVIPKSTN LEHLAENLKI FHFQLTEKDI DDLNSLDKSC HYCWNPECVI
//
