ID T1XCL9_VARPD Unreviewed; 234 AA.
AC T1XCL9;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 24-JAN-2024, entry version 34.
DE RecName: Full=(5-formylfuran-3-yl)methyl phosphate synthase {ECO:0000256|ARBA:ARBA00012553};
DE EC=4.2.3.153 {ECO:0000256|ARBA:ARBA00012553};
DE AltName: Full=4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate synthase {ECO:0000256|ARBA:ARBA00032523};
GN ORFNames=VAPA_1c31370 {ECO:0000313|EMBL:AGU50226.1};
OS Variovorax paradoxus B4.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Comamonadaceae; Variovorax.
OX NCBI_TaxID=1246301 {ECO:0000313|EMBL:AGU50226.1, ECO:0000313|Proteomes:UP000016223};
RN [1] {ECO:0000313|EMBL:AGU50226.1, ECO:0000313|Proteomes:UP000016223}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B4 {ECO:0000313|EMBL:AGU50226.1,
RC ECO:0000313|Proteomes:UP000016223};
RA Schuldes J., Brandt U., Hiessl S., Wuebbeler J.H., Thuermer A.,
RA Steinbuechel A., Daniel R.;
RT "Genome sequence of Variovorax paradoxus B4.";
RL Submitted (OCT-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the formation of 4-(hydroxymethyl)-2-
CC furancarboxaldehyde phosphate (4-HFC-P) from two molecules of
CC glyceraldehyde-3-P (GA-3-P). {ECO:0000256|ARBA:ARBA00003810}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 D-glyceraldehyde 3-phosphate = 4-(hydroxymethyl)-2-
CC furancarboxaldehyde phosphate + 2 H2O + phosphate;
CC Xref=Rhea:RHEA:43536, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474,
CC ChEBI:CHEBI:59776, ChEBI:CHEBI:83407; EC=4.2.3.153;
CC Evidence={ECO:0000256|ARBA:ARBA00001294};
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DR EMBL; CP003911; AGU50226.1; -; Genomic_DNA.
DR RefSeq; WP_021007703.1; NC_022247.1.
DR AlphaFoldDB; T1XCL9; -.
DR KEGG; vpd:VAPA_1c31370; -.
DR PATRIC; fig|1246301.3.peg.3171; -.
DR HOGENOM; CLU_068659_0_0_4; -.
DR OrthoDB; 2111523at2; -.
DR Proteomes; UP000016223; Chromosome 1.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR InterPro; IPR007565; 4HFCP_synth.
DR Pfam; PF04476; 4HFCP_synth; 1.
DR PIRSF; PIRSF015957; UCP015957; 1.
PE 4: Predicted;
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Schiff base {ECO:0000256|ARBA:ARBA00023270}.
FT ACT_SITE 29
FT /note="Schiff-base intermediate with substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR015957-1"
FT ACT_SITE 91
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR015957-1"
SQ SEQUENCE 234 AA; 23789 MW; 9B78BB3FA41CAE40 CRC64;
MTMRMLVSVR SVDEALLAAR GGADFIDLKE PNEGALGGLP VATIGTVVGA LRAHGISLPV
SATIGDLPMH ALDGILAQVD AVGACGVDYV KVGIERGPEA FAVLDALAAC NWPVVPVFIA
DHGLDAALTA HACTLGFPGL MVDTADKQAG SLFDAVPMVE LRAFLAQVRA AGRMAGVAGA
LRTAHVPLLQ SLAPDFAGFR SAVCVGDRKA ALSPERLAAL AVLLHGEAVE PASA
//
