ID T5A654_OPHSC Unreviewed; 687 AA.
AC T5A654;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=aspartate--tRNA ligase {ECO:0000256|ARBA:ARBA00012841};
DE EC=6.1.1.12 {ECO:0000256|ARBA:ARBA00012841};
GN ORFNames=OCS_06214 {ECO:0000313|EMBL:EQK98073.1};
OS Ophiocordyceps sinensis (strain Co18 / CGMCC 3.14243) (Yarsagumba
OS caterpillar fungus) (Hirsutella sinensis).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Ophiocordycipitaceae; Ophiocordyceps.
OX NCBI_TaxID=911162 {ECO:0000313|EMBL:EQK98073.1, ECO:0000313|Proteomes:UP000019374};
RN [1] {ECO:0000313|EMBL:EQK98073.1, ECO:0000313|Proteomes:UP000019374}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Co18 / CGMCC 3.14243 {ECO:0000313|Proteomes:UP000019374};
RC TISSUE=Fruit-body {ECO:0000313|EMBL:EQK98073.1};
RX DOI=10.1007/s11434-013-5929-5;
RA Hu X., Zhang Y., Xiao G., Zheng P., Xia Y., Zhang X., St Leger R.J.,
RA Liu X., Wang C.;
RT "Genome survey uncovers the secrets of sex and lifestyle in caterpillar
RT fungus.";
RL Chin. Sci. Bull. 58:2846-2854(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-
CC aspartyl-tRNA(Asp); Xref=Rhea:RHEA:19649, Rhea:RHEA-COMP:9660,
CC Rhea:RHEA-COMP:9678, ChEBI:CHEBI:29991, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:78442, ChEBI:CHEBI:78516,
CC ChEBI:CHEBI:456215; EC=6.1.1.12;
CC Evidence={ECO:0000256|ARBA:ARBA00000225};
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC Type 2 subfamily. {ECO:0000256|ARBA:ARBA00005312}.
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DR EMBL; KE655668; EQK98073.1; -; Genomic_DNA.
DR AlphaFoldDB; T5A654; -.
DR eggNOG; KOG0556; Eukaryota.
DR HOGENOM; CLU_004553_0_1_1; -.
DR Proteomes; UP000019374; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0004815; F:aspartate-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0006422; P:aspartyl-tRNA aminoacylation; IEA:InterPro.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR InterPro; IPR004364; Aa-tRNA-synt_II.
DR InterPro; IPR006195; aa-tRNA-synth_II.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR004523; Asp-tRNA_synthase_2.
DR InterPro; IPR002312; Asp/Asn-tRNA-synth_IIb.
DR InterPro; IPR024320; LPG_synthase_C.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR PANTHER; PTHR43450:SF2; ASPARTATE--TRNA LIGASE; 1.
DR PANTHER; PTHR43450; ASPARTYL-TRNA SYNTHETASE; 1.
DR Pfam; PF09924; LPG_synthase_C; 1.
DR Pfam; PF00152; tRNA-synt_2; 1.
DR PRINTS; PR01042; TRNASYNTHASP.
DR SUPFAM; SSF55681; Class II aaRS and biotin synthetases; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE 3: Inferred from homology;
KW Ligase {ECO:0000313|EMBL:EQK98073.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000019374}.
FT DOMAIN 125..359
FT /note="Aminoacyl-transfer RNA synthetases class-II family
FT profile"
FT /evidence="ECO:0000259|PROSITE:PS50862"
SQ SEQUENCE 687 AA; 77732 MW; 3CC9D1786CDA4E76 CRC64;
MVSWVQKLPA ESLVQVNGTL KAPPEPVRSA SHAGVEVHVH SVHLVNAAHN APFSNYKPPE
SMRNRMSSRI LDLRHPSNQA LFRVRSLVTR NGAAVFKVNY FGRNAFLAQS PQLAKQMSVS
ADFGRVFEVG PVFRAENSNT HRHLTEYTGL DIEMAIEHDY HEVIDVIDEF LKAVFTTVYS
MPELAEVRKR WPSKEFKYLD ETLILDFQDG IKMLREDGRD VEEEEDLATA DEIRLGQLVR
DKYGTDYYVL DKFPASARPF YTHKDPQDPR WTRSFDIFIR GQEICSGGQR IHDAEELRAN
MRAAGMNEDG MEEYMEAFDL GAPPHAGAGL GLERIVAWML ELGDVRYASL FYRDPKSLPE
KAPGLMHPEA DTTKPQPASD LPPVEKLIAN YGDASNTSWL DDRFEIWRHP TGAAVGYVKQ
DKFVMMTGDP LCDRTQYKDV CAAFVKFAVS ELHLVPVWML VSYEVQKILA QQMGWRTLSC
TEEQRVDANK HHNAHDGPKA RRVEREGVKI HEVKADADFI KRADPAIAEW KASRKGKQIH
MTEVRPWVDM EHRRYFAAER DGKVLALVVL AKLSPKHGWQ VKWALDFPGS VNGAIEVLVE
YTLASVTGQV TFGVGVSEKL TPGEHLNGIR ARFLASTYNS IVDSLGLRRK AGFRSKFGAL
GEEVYICYPK HGVGLRDLTH IIKFFQD
//
