ID TEE6_STRP6 Reviewed; 537 AA.
AC P18481; Q5XE68;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 2.
DT 24-JAN-2024, entry version 133.
DE RecName: Full=Trypsin-resistant surface T6 protein;
DE AltName: Full=T6 antigen;
DE Flags: Precursor;
GN Name=tee6; OrderedLocusNames=M6_Spy0160;
OS Streptococcus pyogenes serotype M6 (strain ATCC BAA-946 / MGAS10394).
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
OC Streptococcus.
OX NCBI_TaxID=286636;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=D471 / Serotype M6;
RX PubMed=2188957; DOI=10.1128/jb.172.6.3310-3317.1990;
RA Schneewind O., Jones K.F., Fischetti V.A.;
RT "Sequence and structural characteristics of the trypsin-resistant T6
RT surface protein of group A streptococci.";
RL J. Bacteriol. 172:3310-3317(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11854196; DOI=10.1128/iai.70.3.1159-1167.2002;
RA Bessen D.E., Kalia A.;
RT "Genomic localization of a T serotype locus to a recombinatorial zone
RT encoding extracellular matrix-binding proteins in Streptococcus pyogenes.";
RL Infect. Immun. 70:1159-1167(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-946 / MGAS10394;
RX PubMed=15272401; DOI=10.1086/422697;
RA Banks D.J., Porcella S.F., Barbian K.D., Beres S.B., Philips L.E.,
RA Voyich J.M., DeLeo F.R., Martin J.M., Somerville G.A., Musser J.M.;
RT "Progress toward characterization of the group A Streptococcus metagenome:
RT complete genome sequence of a macrolide-resistant serotype M6 strain.";
RL J. Infect. Dis. 190:727-738(2004).
CC -!- INTERACTION:
CC P18481; Q5XE69: M6_Spy0159; NbExp=2; IntAct=EBI-5260395, EBI-5260633;
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000255|PROSITE-
CC ProRule:PRU00477}; Peptidoglycan-anchor {ECO:0000255|PROSITE-
CC ProRule:PRU00477}.
CC -!- MISCELLANEOUS: More than 25 serologically different T antigens are
CC found to be expressed independently of M protein, but certain T
CC proteins occur only in association with specific M serotypes (i.e. T6
CC protein is always found on M6 streptococcal strains).
CC -!- MISCELLANEOUS: The T protein is exposed on the cell surface when
CC fibrillar M protein is enzymatically removed.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAT86295.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; M32978; AAA27019.1; -; Genomic_DNA.
DR EMBL; AY049087; AAL11467.1; -; Genomic_DNA.
DR EMBL; CP000003; AAT86295.1; ALT_INIT; Genomic_DNA.
DR PIR; A35400; A35400.
DR PDB; 4P0D; X-ray; 1.90 A; A=21-508.
DR PDBsum; 4P0D; -.
DR AlphaFoldDB; P18481; -.
DR SMR; P18481; -.
DR IntAct; P18481; 1.
DR KEGG; spa:M6_Spy0160; -.
DR HOGENOM; CLU_028873_3_0_9; -.
DR Proteomes; UP000001167; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.740; -; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR032334; GramPos_pilinBB.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR019931; LPXTG_anchor.
DR InterPro; IPR041033; Prealbumin-like.
DR InterPro; IPR041184; T6_Ig-like.
DR NCBIfam; TIGR01167; LPXTG_anchor; 1.
DR Pfam; PF00746; Gram_pos_anchor; 1.
DR Pfam; PF16569; GramPos_pilinBB; 1.
DR Pfam; PF17802; SpaA; 1.
DR Pfam; PF18002; T6_Ig_like; 1.
DR SUPFAM; SSF49478; Cna protein B-type domain; 1.
DR PROSITE; PS50847; GRAM_POS_ANCHORING; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell wall; Peptidoglycan-anchor; Secreted; Signal.
FT SIGNAL 1..22
FT CHAIN 23..507
FT /note="Trypsin-resistant surface T6 protein"
FT /id="PRO_0000005671"
FT PROPEP 508..537
FT /note="Removed by sortase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT /id="PRO_0000005672"
FT REGION 310..330
FT /note="Hydrophilic"
FT MOTIF 504..508
FT /note="LPXTG sorting signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT MOD_RES 507
FT /note="Pentaglycyl murein peptidoglycan amidated threonine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00477"
FT CONFLICT 318
FT /note="K -> R (in Ref. 1 and 2)"
FT /evidence="ECO:0000305"
FT STRAND 26..32
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 39..50
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 56..62
FT /evidence="ECO:0007829|PDB:4P0D"
FT HELIX 75..87
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 88..90
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 93..98
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 104..120
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 131..136
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 158..161
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 168..175
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 177..179
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 182..184
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 190..198
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 211..216
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 221..232
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 244..247
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 250..257
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 259..266
FT /evidence="ECO:0007829|PDB:4P0D"
FT HELIX 268..271
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 277..284
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 299..306
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 316..318
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 326..331
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 334..336
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 338..346
FT /evidence="ECO:0007829|PDB:4P0D"
FT TURN 347..349
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 357..362
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 367..372
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 377..380
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 386..395
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 407..412
FT /evidence="ECO:0007829|PDB:4P0D"
FT TURN 413..415
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 418..420
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 428..431
FT /evidence="ECO:0007829|PDB:4P0D"
FT HELIX 433..435
FT /evidence="ECO:0007829|PDB:4P0D"
FT HELIX 437..439
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 444..447
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 450..453
FT /evidence="ECO:0007829|PDB:4P0D"
FT HELIX 454..456
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 464..470
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 480..482
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 487..491
FT /evidence="ECO:0007829|PDB:4P0D"
FT STRAND 496..498
FT /evidence="ECO:0007829|PDB:4P0D"
SQ SEQUENCE 537 AA; 57647 MW; 70F7251EEFDBF644 CRC64;
MLACLAILAV VGLGMTRVSA LSKDDTAQLK ITNIEGGPTV TLYKIGEGVY NTNGDSFINF
KYAEGVSLTE TGPTSQEITT IANGINTGKI KPFSTENVSI SNGTATYNAR GASVYIALLT
GATDGRTYNP ILLAASYNGE GNLVTKNIDS KSNYLYGQTS VAKSSLPSIT KKVTGTIDDV
NKKTTSLGSV LSYSLTFELP SYTKEAVNKT VYVSDNMSEG LTFNFNSLTV EWKGKMANIT
EDGSVMVENT KIGIAKEVNN GFNLSFIYDS LESISPNISY KAVVNNKAIV GEEGNPNKAE
FFYSNNPTKG NTYDNLDKKP DKGNGITSKE DSKIVYTYQI AFRKVDSVSK TPLIGAIFGV
YDTSNKLIDI VTTNKNGYAI STQVSSGKYK IKELKAPKGY SLNTETYEIT ANWVTATVKT
SANSKSTTYT SDKNKATDNS EQVGWLKNGI FYSIDSRPTG NDVKEAYIES TKALTDGTTF
SKSNEGSGTV LLETDIPNTK LGELPSTGSI GTYLFKAIGS AAMIGAIGIY IVKRRKA
//
