ID TMG10_METTH Reviewed; 336 AA.
AC O26820;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 27-MAR-2024, entry version 124.
DE RecName: Full=tRNA (guanine(10)-N2)-dimethyltransferase;
DE EC=2.1.1.213;
DE AltName: Full=tRNA:G10 dimethyltransferase;
GN Name=trmG10; OrderedLocusNames=MTH_724;
OS Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM
OS 10044 / NBRC 100330 / Delta H) (Methanobacterium thermoautotrophicum).
OC Archaea; Euryarchaeota; Methanomada group; Methanobacteria;
OC Methanobacteriales; Methanobacteriaceae; Methanothermobacter.
OX NCBI_TaxID=187420;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H;
RX PubMed=9371463; DOI=10.1128/jb.179.22.7135-7155.1997;
RA Smith D.R., Doucette-Stamm L.A., Deloughery C., Lee H.-M., Dubois J.,
RA Aldredge T., Bashirzadeh R., Blakely D., Cook R., Gilbert K., Harrison D.,
RA Hoang L., Keagle P., Lumm W., Pothier B., Qiu D., Spadafora R., Vicare R.,
RA Wang Y., Wierzbowski J., Gibson R., Jiwani N., Caruso A., Bush D.,
RA Safer H., Patwell D., Prabhakar S., McDougall S., Shimer G., Goyal A.,
RA Pietrovski S., Church G.M., Daniels C.J., Mao J.-I., Rice P., Noelling J.,
RA Reeve J.N.;
RT "Complete genome sequence of Methanobacterium thermoautotrophicum deltaH:
RT functional analysis and comparative genomics.";
RL J. Bacteriol. 179:7135-7155(1997).
CC -!- FUNCTION: Catalyzes the adenosylmethionine-dependent methylation of the
CC exocyclic amino group (N(2)) of guanosine at position 10 of various
CC tRNAs. Acts via a two-step process that leads to the formation of
CC either N(2)-monomethyl (m(2)G) or N(2)-dimethylguanosine (m(2)(2)G) (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(10) in tRNA + 2 S-adenosyl-L-methionine = 2 H(+) +
CC N(2)-dimethylguanosine(10) in tRNA + 2 S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43124, Rhea:RHEA-COMP:10355, Rhea:RHEA-COMP:10358,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:74269, ChEBI:CHEBI:74513; EC=2.1.1.213;
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily. Trm-G10
CC family. {ECO:0000305}.
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DR EMBL; AE000666; AAB85229.1; -; Genomic_DNA.
DR PIR; G69196; G69196.
DR AlphaFoldDB; O26820; -.
DR SMR; O26820; -.
DR STRING; 187420.MTH_724; -.
DR PaxDb; 187420-MTH_724; -.
DR EnsemblBacteria; AAB85229; AAB85229; MTH_724.
DR KEGG; mth:MTH_724; -.
DR PATRIC; fig|187420.15.peg.709; -.
DR HOGENOM; CLU_057819_1_0_2; -.
DR InParanoid; O26820; -.
DR Proteomes; UP000005223; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0160101; F:tRNA (guanine(10)-N2)-dimethyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0160102; F:tRNA (guanine(10)-N2)-methyltransferase activity; IEA:InterPro.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
DR GO; GO:0030488; P:tRNA methylation; IEA:InterPro.
DR CDD; cd02440; AdoMet_MTases; 1.
DR CDD; cd11715; THUMP_AdoMetMT; 1.
DR Gene3D; 3.30.2130.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR000241; RlmKL-like_Mtase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR004114; THUMP_dom.
DR InterPro; IPR005885; TrmG10.
DR NCBIfam; TIGR01177; TIGR01177 family methyltransferase; 1.
DR PANTHER; PTHR14911; THUMP DOMAIN-CONTAINING; 1.
DR PANTHER; PTHR14911:SF13; TRNA (GUANINE(6)-N2)-METHYLTRANSFERASE THUMP3; 1.
DR Pfam; PF02926; THUMP; 1.
DR Pfam; PF01170; UPF0020; 1.
DR PIRSF; PIRSF017259; tRNA_mtfrase_TRM11; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SMART; SM00981; THUMP; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR SUPFAM; SSF143437; THUMP domain-like; 1.
DR PROSITE; PS51165; THUMP; 1.
DR PROSITE; PS01261; UPF0020; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Methyltransferase; Reference proteome; RNA-binding;
KW S-adenosyl-L-methionine; Transferase; tRNA processing; tRNA-binding.
FT CHAIN 1..336
FT /note="tRNA (guanine(10)-N2)-dimethyltransferase"
FT /id="PRO_0000140482"
FT DOMAIN 50..147
FT /note="THUMP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00529"
SQ SEQUENCE 336 AA; 38020 MW; D305B3FFADE1F5CC CRC64;
MEMMVLLSQE HPELPSAELR SVLMSEGIDF SVIESGSGYE VIDAPRSTWK ILKKRLAYAH
EISEVIGYAA ADDLDDAAEE IDWSKYVRES FAVRIRKLCG DVDSRTLERT IGAIIKEDTG
LKVDLEKPWT LIRPVLINDR FILTRRLAVI SKEHFNQARP HKRPFFYPGS MSPKLARCMV
NLSGVKAGDR ILDPFCGTGG ILIEAGLMGV RVVGADIDWR MVEGTRENLQ HYGITDFEVI
RSDARDLRLD EKVDAIVTDP PYGISASTAG EKSEKLYREF LDSAHSNLAE GGMICMAAPH
YLDLESLIDE RFSIRERYSM RMHRSLTRVI RVIERV
//
