ID U1LGG7_9BACL Unreviewed; 562 AA.
AC U1LGG7;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=Rqc2 homolog RqcH {ECO:0000256|HAMAP-Rule:MF_00844};
DE Short=RqcH {ECO:0000256|HAMAP-Rule:MF_00844};
GN Name=rqcH {ECO:0000256|HAMAP-Rule:MF_00844};
GN ORFNames=M467_04030 {ECO:0000313|EMBL:ERG66443.1};
OS Exiguobacterium chiriqhucha RW-2.
OC Bacteria; Bacillota; Bacilli; Bacillales;
OC Bacillales Family XII. Incertae Sedis; Exiguobacterium.
OX NCBI_TaxID=1345023 {ECO:0000313|EMBL:ERG66443.1, ECO:0000313|Proteomes:UP000016464};
RN [1] {ECO:0000313|EMBL:ERG66443.1, ECO:0000313|Proteomes:UP000016464}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RW-2 {ECO:0000313|EMBL:ERG66443.1,
RC ECO:0000313|Proteomes:UP000016464};
RX PubMed=23929485;
RA White R.A.III., Grassa C.J., Suttle C.A.;
RT "Draft Genome Sequence of Exiguobacterium pavilionensis Strain RW-2, with
RT Wide Thermal, Salinity, and pH Tolerance, Isolated from Modern Freshwater
RT Microbialites.";
RL Genome Announc. 1:e00597-e00513(2013).
CC -!- FUNCTION: Part of the ribosome quality control system (RQC). Recruits
CC Ala-charged tRNA and directs the elongation of stalled nascent chains
CC on 50S ribosomal subunits, leading to non-templated C-terminal Ala
CC extensions (Ala tail). The Ala tail promotes nascent chain degradation.
CC May add between 1 and at least 8 Ala residues. Binds to stalled 50S
CC ribosomal subunits. {ECO:0000256|HAMAP-Rule:MF_00844}.
CC -!- SUBUNIT: Associates with stalled 50S ribosomal subunits.
CC {ECO:0000256|HAMAP-Rule:MF_00844}.
CC -!- SIMILARITY: Belongs to the NEMF family. {ECO:0000256|HAMAP-
CC Rule:MF_00844}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ERG66443.1}.
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DR EMBL; ATCL01000020; ERG66443.1; -; Genomic_DNA.
DR RefSeq; WP_021067069.1; NZ_ATCL01000020.1.
DR AlphaFoldDB; U1LGG7; -.
DR STRING; 1385984.GCA_000702565_01865; -.
DR PATRIC; fig|1345023.5.peg.1912; -.
DR eggNOG; COG1293; Bacteria.
DR OrthoDB; 9766163at2; -.
DR Proteomes; UP000016464; Unassembled WGS sequence.
DR GO; GO:0043023; F:ribosomal large subunit binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0072344; P:rescue of stalled ribosome; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.8.50; -; 1.
DR Gene3D; 3.40.970.40; fibrinogen binding protein from staphylococcus aureus domain like; 1.
DR Gene3D; 2.30.310.10; ibrinogen binding protein from staphylococcus aureus domain; 1.
DR HAMAP; MF_00844_B; RqcH_B; 1.
DR InterPro; IPR008532; NFACT_RNA-bd.
DR InterPro; IPR043682; RqcH_bacterial.
DR PANTHER; PTHR15239; NUCLEAR EXPORT MEDIATOR FACTOR NEMF; 1.
DR PANTHER; PTHR15239:SF6; RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF; 1.
DR Pfam; PF05670; NFACT-R_1; 1.
DR Pfam; PF18297; NFACT-R_2; 1.
DR Pfam; PF05833; NFACT_N; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|HAMAP-Rule:MF_00844};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00844}; Reference proteome {ECO:0000313|Proteomes:UP000016464};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|HAMAP-
KW Rule:MF_00844};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|HAMAP-
KW Rule:MF_00844}.
FT DOMAIN 446..534
FT /note="NFACT RNA-binding"
FT /evidence="ECO:0000259|Pfam:PF05670"
FT COILED 280..315
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00844"
SQ SEQUENCE 562 AA; 63967 MW; 276AC916BDB60E67 CRC64;
MAYDGLMTYR VVTELQALVG GRINRVHQPY SLDLMIQVRS NRQSVQLLVS ANAMYARLQL
TDNPTKNPQE PPMFCMMLRK HIEGGFITAI EQVGRDRVIV LSIRSRNELG DEENKKVYIE
LMGRHSNVVL TNEDGKILDA IKHLPPSQNT YRTIMPGSEY LLPPAQDKCD PLTEREAGLK
RIDWNAGSLD KQLVASFAGL SPQIAQEIVH RAKLPNRASI EESFVSVMSD LEGPYVFQQL
ASGKERFSPI VLTSGEVTSE ERYETSKDVL DRFFYEKANR DRVKQQAHDV ERLLKSELEK
NLLKRKRLLD DLKATERSDE LQKYGELLTT YLFQLEKGMT VAHVVDYYDE EGGMIDIPLN
KLKTPNENAQ AYYKKYNKLK VAKVEVQKQI ELNDAEIEYL ETLVAQLDVA SPADIVEIRE
ELAEGGYMKQ RNQKKKQTKK ISLEAYTSST GTEFFVGKNN TQNDHLTFKF ARRDEIWLHV
KDIPGSHVII RSSEPDETTL LEAATVAAYF SKARASSGVP VDYTKARYVK KPSGAKPGFV
IYTDQQTVYV TPDERLVKSL QQ
//
