ID U2HE28_9BURK Unreviewed; 401 AA.
AC U2HE28;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=Methyltransferase {ECO:0000256|RuleBase:RU362026};
DE EC=2.1.1.- {ECO:0000256|RuleBase:RU362026};
GN ORFNames=L810_6891 {ECO:0000313|EMBL:ERJ38674.1};
OS Burkholderia sp. AU4i.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Burkholderia.
OX NCBI_TaxID=1335308 {ECO:0000313|EMBL:ERJ38674.1, ECO:0000313|Proteomes:UP000016616};
RN [1] {ECO:0000313|EMBL:ERJ38674.1, ECO:0000313|Proteomes:UP000016616}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AU4i {ECO:0000313|EMBL:ERJ38674.1,
RC ECO:0000313|Proteomes:UP000016616};
RA Devi U., Khatri I., Kumar N., Kumar L., Singh D., Sharma D.,
RA Subramanian S., Saini A.K.;
RT "Genome Assembly of Burkholderia vietnamiensis AU4i.";
RL Submitted (MAY-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = an N(4)-
CC methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:16857, Rhea:RHEA-COMP:11369, Rhea:RHEA-COMP:13674,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:85452, ChEBI:CHEBI:137933; EC=2.1.1.113;
CC Evidence={ECO:0000256|ARBA:ARBA00001893};
CC -!- SIMILARITY: Belongs to the N(4)/N(6)-methyltransferase family. N(4)
CC subfamily. {ECO:0000256|ARBA:ARBA00010203}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ERJ38674.1}.
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DR EMBL; ASSI01000060; ERJ38674.1; -; Genomic_DNA.
DR AlphaFoldDB; U2HE28; -.
DR PATRIC; fig|1335308.3.peg.2907; -.
DR Proteomes; UP000016616; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0015667; F:site-specific DNA-methyltransferase (cytosine-N4-specific) activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR002941; DNA_methylase_N4/N6.
DR InterPro; IPR017985; MeTrfase_CN4_CS.
DR InterPro; IPR025745; Mrr-like_N_dom.
DR InterPro; IPR001091; RM_Methyltransferase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR Pfam; PF14338; Mrr_N; 1.
DR Pfam; PF01555; N6_N4_Mtase; 1.
DR PRINTS; PR00508; S21N4MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00093; N4_MTASE; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:ERJ38674.1};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:ERJ38674.1}.
FT DOMAIN 1..85
FT /note="Restriction system protein Mrr-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF14338"
FT DOMAIN 121..372
FT /note="DNA methylase N-4/N-6"
FT /evidence="ECO:0000259|Pfam:PF01555"
SQ SEQUENCE 401 AA; 44272 MW; 2E8FEEA536DDE342 CRC64;
MLPLLDCIEN GGGKAHTHDL YDAIALKCGV PEVEQQETTL LAGRNVNVYQ RSVRWAQQRA
RLMGLINASG RGEWELTGKG RDALRQAAPG IVITIFTTSK GVALFGEAKE AVAHIDDGSL
RLILTSPPYP LLREKQYGNK PVEEYLDWLM RIAELWPQKL AGDGSVVLNL GDVFQSGVPM
LSTYQERLII RLEDDLGWKL CSRYAWHNPS KMPAPAEWVT VRRVRVKPSL EQIYWLAPNG
EPYADNSQVL VPYSESMRSR IAAGGEKGAV RPSGHELSAG AFDVDNGGAI PHTLIVAANT
ESNSHYMKAC REQNLPVHPA RFPGAVPEHF IKLCTMEGDT VYDPFSGSFK TGAEAERLGR
NWIGSELMLD YVLGGANRFP DAVLTRQGID DFRRAADLFA C
//
