ID U2NXL6_ENTFC Unreviewed; 380 AA.
AC U2NXL6;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=I131_07135 {ECO:0000313|EMBL:ERK33818.1};
OS Enterococcus faecium CRL1879.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
OC Enterococcus.
OX NCBI_TaxID=1295527 {ECO:0000313|EMBL:ERK33818.1, ECO:0000313|Proteomes:UP000016582};
RN [1] {ECO:0000313|EMBL:ERK33818.1, ECO:0000313|Proteomes:UP000016582}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CRL1879 {ECO:0000313|EMBL:ERK33818.1,
RC ECO:0000313|Proteomes:UP000016582};
RX PubMed=23868133;
RA Suarez N.E., Saavedra L., Slozilova I., Bonacina J., Demnerova K.,
RA Sesma F.;
RT "Draft Genome Sequence of Enterococcus faecium Strain CRL 1879, Isolated
RT from a Northwestern Argentinian Artisanal Cheese.";
RL Genome Announc. 1:E00514-13(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ERK33818.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AOUK01000047; ERK33818.1; -; Genomic_DNA.
DR AlphaFoldDB; U2NXL6; -.
DR Proteomes; UP000016582; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR004546; Restrct_endonuc_T1M.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR NCBIfam; TIGR00497; hsdM; 1.
DR PANTHER; PTHR42933:SF1; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); 1.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:ERK33818.1};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 5..161
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 170..379
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 380 AA; 42994 MW; 956B5B923E9C4629 CRC64;
MGAELNQKLF SAADNLRSKM DASEYKNYLL GLIFYKYLSD RLLEQVVLLA DESLEEYDTV
SKQTMLYREL LSDEESKEDL IATIVDILGY AIAPEYLFNV LADQAKQATF QLNDLNKAFV
QLASTYNQFN GLFDDVDLQS KKLGTDEQQR NVTITEVIKK LNDVEVLGHD GDVIGDAYEF
LISQFASEAG KKAGEFYTPH MVSDMMAQIV TLDQKERRFF SVFDPTMGSG SLMLNVRNYL
THPDNVKYHG QELNTTTYNL AKMNLILHGV DAEEMNLRNG DTLNKDWPTD EPYTFDAVVM
NPPYSANWSA DTTFLDDSRF NRYGKLAPKS KADFAFLLHG FYHLKETGTM AIVLPHGVLF
RGAAEGVIRQ KLLEDGSIYA
//
