ID U3B2M5_9VIBR Unreviewed; 372 AA.
AC U3B2M5;
DT 13-NOV-2013, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2013, sequence version 1.
DT 27-MAR-2024, entry version 47.
DE RecName: Full=aminomethyltransferase {ECO:0000256|ARBA:ARBA00012616};
DE EC=2.1.2.10 {ECO:0000256|ARBA:ARBA00012616};
DE AltName: Full=Glycine cleavage system T protein {ECO:0000256|ARBA:ARBA00031395};
GN Name=gcvT {ECO:0000313|EMBL:GAD80205.1};
GN ORFNames=VEZ01S_26_00440 {ECO:0000313|EMBL:GAD80205.1};
OS Vibrio ezurae NBRC 102218.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=1219080 {ECO:0000313|EMBL:GAD80205.1, ECO:0000313|Proteomes:UP000016562};
RN [1] {ECO:0000313|EMBL:GAD80205.1, ECO:0000313|Proteomes:UP000016562}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 102218 {ECO:0000313|EMBL:GAD80205.1,
RC ECO:0000313|Proteomes:UP000016562};
RA Yoshida I., Hosoyama A., Numata M., Hashimoto M., Hosoyama Y.,
RA Tsuchikane K., Noguchi M., Hirakata S., Ichikawa N., Ohji S., Yamazoe A.,
RA Fujita N.;
RT "Whole genome shotgun sequence of Vibrio ezurae NBRC 102218.";
RL Submitted (SEP-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + N(6)-[(R)-S(8)-
CC aminomethyldihydrolipoyl]-L-lysyl-[protein] = (6R)-5,10-
CC methylene-5,6,7,8-tetrahydrofolate + N(6)-[(R)-dihydrolipoyl]-L-
CC lysyl-[protein] + NH4(+); Xref=Rhea:RHEA:16945, Rhea:RHEA-COMP:10475,
CC Rhea:RHEA-COMP:10492, ChEBI:CHEBI:15636, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:57453, ChEBI:CHEBI:83100, ChEBI:CHEBI:83143; EC=2.1.2.10;
CC Evidence={ECO:0000256|ARBA:ARBA00043710};
CC -!- SIMILARITY: Belongs to the GcvT family.
CC {ECO:0000256|ARBA:ARBA00008609}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAD80205.1}.
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DR EMBL; BATM01000026; GAD80205.1; -; Genomic_DNA.
DR RefSeq; WP_021713913.1; NZ_BATM01000026.1.
DR AlphaFoldDB; U3B2M5; -.
DR STRING; 1219080.VEZ01S_26_00440; -.
DR eggNOG; COG0404; Bacteria.
DR OrthoDB; 9774591at2; -.
DR Proteomes; UP000016562; Unassembled WGS sequence.
DR GO; GO:0004047; F:aminomethyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0008483; F:transaminase activity; IEA:UniProtKB-KW.
DR GO; GO:0006546; P:glycine catabolic process; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR Gene3D; 2.40.30.110; Aminomethyltransferase beta-barrel domains; 1.
DR Gene3D; 3.30.70.1400; Aminomethyltransferase beta-barrel domains; 1.
DR Gene3D; 4.10.1250.10; Aminomethyltransferase fragment; 1.
DR InterPro; IPR006223; GCS_T.
DR InterPro; IPR028896; GCST/YgfZ/DmdA.
DR InterPro; IPR013977; GCV_T_C.
DR InterPro; IPR006222; GCV_T_N.
DR InterPro; IPR029043; GcvT/YgfZ_C.
DR InterPro; IPR027266; TrmE/GcvT_dom1.
DR NCBIfam; TIGR00528; gcvT; 1.
DR PANTHER; PTHR43757; AMINOMETHYLTRANSFERASE; 1.
DR PANTHER; PTHR43757:SF2; AMINOMETHYLTRANSFERASE, MITOCHONDRIAL; 1.
DR Pfam; PF01571; GCV_T; 1.
DR Pfam; PF08669; GCV_T_C; 1.
DR PIRSF; PIRSF006487; GcvT; 1.
DR SUPFAM; SSF101790; Aminomethyltransferase beta-barrel domain; 1.
DR SUPFAM; SSF103025; Folate-binding domain; 1.
PE 3: Inferred from homology;
KW Aminotransferase {ECO:0000256|ARBA:ARBA00022576};
KW Methyltransferase {ECO:0000313|EMBL:GAD80205.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000016562};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:GAD80205.1}.
FT DOMAIN 10..254
FT /note="Aminomethyltransferase folate-binding"
FT /evidence="ECO:0000259|Pfam:PF01571"
FT DOMAIN 287..365
FT /note="Glycine cleavage T-protein C-terminal barrel"
FT /evidence="ECO:0000259|Pfam:PF08669"
SQ SEQUENCE 372 AA; 40133 MW; 2523ED39DF63BAB3 CRC64;
MTEVLLKTPL HAVHVEQGAK MVPFAGYDMP VQYPLGVKKE HLHTRDAAGL FDVSHMGQLR
LKGDNAAATL EALVPVDIID LPNGNQRYAF FTNEQGGIMD DLMVANLGDH LFVVVNAACK
EQDIAHLQAN LLEGVELEVI EDRALLALQG PKAVDVLATL NPAVKDMVFM DVARLELLGV
ECIVSRSGYT GEDGFEISVP NAQAAALANT LTDSDLVEWI GLGARDSLRL ECGLCLYGHD
LDTTTTPVEA SLLWGISKIR RADGQRAGGF PGADIILEQI ATKDVKRKRV GLVGQTKAPV
REGCKLFDAN DVEVGIVTSG TAGPNAGKPV SMAYVRSDLM AVGTEIFAEV RGKKLPMTVE
KMPFVPQRYY RG
//
