ID   U5HTH1_MANES            Unreviewed;       965 AA.
AC   U5HTH1;
DT   22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT   22-JAN-2014, sequence version 1.
DT   27-MAR-2024, entry version 24.
DE   RecName: Full=phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00012305};
DE            EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305};
GN   Name=PEPC {ECO:0000313|EMBL:AFN70424.1};
OS   Manihot esculenta (Cassava) (Jatropha manihot).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Malpighiales; Euphorbiaceae; Crotonoideae; Manihoteae;
OC   Manihot.
OX   NCBI_TaxID=3983 {ECO:0000313|EMBL:AFN70424.1};
RN   [1] {ECO:0000313|EMBL:AFN70424.1}
RP   NUCLEOTIDE SEQUENCE.
RC   TISSUE=Leaf {ECO:0000313|EMBL:AFN70424.1};
RA   Zhang Y., Chen X., Lu C., Wang W.-Q.;
RT   "Cloning and Expression Analysis of Phosphoenolpyruvate Carboxylase Gene
RT   pepc from Cassava (Manihot esculenta Crantz).";
RL   J Trop Subtrop Botany 20:369-375(2012).
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC       {ECO:0000256|ARBA:ARBA00008346}.
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DR   EMBL; JN387052; AFN70424.1; -; mRNA.
DR   AlphaFoldDB; U5HTH1; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 1.
DR   HAMAP; MF_00595; PEPcase_type1; 1.
DR   InterPro; IPR021135; PEP_COase.
DR   InterPro; IPR022805; PEP_COase_bac/pln-type.
DR   InterPro; IPR018129; PEP_COase_Lys_AS.
DR   InterPro; IPR033129; PEPCASE_His_AS.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR   PANTHER; PTHR30523:SF47; PHOSPHOENOLPYRUVATE CARBOXYLASE 2; 1.
DR   Pfam; PF00311; PEPcase; 1.
DR   PRINTS; PR00150; PEPCARBXLASE.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR   PROSITE; PS00781; PEPCASE_1; 1.
DR   PROSITE; PS00393; PEPCASE_2; 1.
PE   2: Evidence at transcript level;
KW   Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW   Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW   Pyruvate {ECO:0000313|EMBL:AFN70424.1}.
FT   ACT_SITE        172
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10111"
FT   ACT_SITE        600
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU10112"
SQ   SEQUENCE   965 AA;  110360 MW;  E7600C1E126E3FEB CRC64;
     MAARNLEKMA SIDAQLRLLA PRKVSEDDKL VEYDALLLDR FLDISQDLHG EEIRETVQDC
     YELSAEYEGK HDPQKLEELG KVLTSLDPGD SIVVAKSFSH MLNLANLAEE VQIAYGRRIK
     LKKGDFADEN SATTESDIEE TLKRLVVQLK KSPEEVFDAL KNQTVDLVLT AHPTQSVRRS
     LLQKHARIRN CLTQLYAKDI TPDDKQELDE ALQREIQAAF RTDEIRRTPP TPQDEMRAGM
     SYFHETIWKG VPKFLRRVDT ALKNIGINER IPYNAPLIQF SSWMGGDRDG NPRVTPEVTR
     DVCLLARMMA ANLYFSQIED LMFELSMWRC NDELRVHADE LHRSSRRDAK HYIEFWKQIP
     ASEPYRVILG DVRDKLYNTR ERSRHLLVNG ISDIPEEATF TNVEQFLQPL ELCYRSLCAC
     GDRPIADGSL LDFLRQVSTF GLSLVRLDIR QESDRHTDVL DAITKYLGIG SYREWSEESR
     QEWLLSELRG KRPLFGPDLP KTEEIADVLD TFHVIAELPP DNFGAYIISM ATAPSDVLAV
     ELLQRECHVK QPLRVVPLFE KLADLEAAPA AVARLFSVDW YRNRINGKQE VMIGYSDSGK
     DAGRLSAAWQ LYKAQEELVK VAKQYGVKLT MFHGRGGTVG RGGGPTHLAI LSQPPDTIHG
     SLRVTVQGEV IEQSFGEEHL CFRTLQRFTA ATLEHGMHPP ISPKPEWRAL MDEMAIIATK
     EYRSIVFQEP RFVEYFRLAT PELEYGRMNI GSRPSKRKPS GGIESLRAIP WIFAWTQTRF
     HLPVWLGFGA AFRHVVEKDI KNLQMLQEMY NQWPFFRVTI DLVEMVFAKG DPGIAALYDK
     LLVSKELWPF GERLRANYEE TKSFLLKIAG HKDLLEGDPY LKQRLRLRDA YITTLNVCQV
     YTLKRIRDPD YYVTVRPHLS KEYMESTKPA AELVKLNPTS EYAPGLEDTL ILTMKGIAAG
     MQNTG
//