ID UXAA_BACSU Reviewed; 497 AA.
AC O34673;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 16-JUN-2009, sequence version 2.
DT 27-MAR-2024, entry version 124.
DE RecName: Full=Altronate dehydratase;
DE EC=4.2.1.7;
DE AltName: Full=D-altronate hydro-lyase;
GN Name=uxaA; Synonyms=yjmJ; OrderedLocusNames=BSU12390;
OS Bacillus subtilis (strain 168).
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROBABLE OPERON STRUCTURE.
RC STRAIN=168;
RX PubMed=9579062; DOI=10.1099/00221287-144-4-877;
RA Rivolta C., Soldo B., Lazarevic V., Joris B., Mauel C., Karamata D.;
RT "A 35.7 kb DNA fragment from the Bacillus subtilis chromosome containing a
RT putative 12.3 kb operon involved in hexuronate catabolism and a perfectly
RT symmetrical hypothetical catabolite-responsive element.";
RL Microbiology 144:877-884(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION TO 19.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
RN [4]
RP PROBABLE OPERON STRUCTURE, AND INDUCTION.
RC STRAIN=168 / MB24;
RX PubMed=9882655; DOI=10.1128/jb.181.2.426-433.1999;
RA Mekjian K.R., Bryan E.M., Beall B.W., Moran C.P. Jr.;
RT "Regulation of hexuronate utilization in Bacillus subtilis.";
RL J. Bacteriol. 181:426-433(1999).
CC -!- FUNCTION: Catalyzes the dehydration of D-altronate. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-altronate = 2-dehydro-3-deoxy-D-gluconate + H2O;
CC Xref=Rhea:RHEA:15957, ChEBI:CHEBI:15377, ChEBI:CHEBI:17360,
CC ChEBI:CHEBI:57990; EC=4.2.1.7;
CC -!- COFACTOR:
CC Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000250};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC -!- PATHWAY: Carbohydrate metabolism; pentose and glucuronate
CC interconversion.
CC -!- INDUCTION: Induced by galacturonate, repressed by glucose.
CC {ECO:0000305|PubMed:9882655}.
CC -!- MISCELLANEOUS: Last gene in the exu locus which is required for
CC galacturonate utilization.
CC -!- SIMILARITY: Belongs to the UxaA family. {ECO:0000305}.
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DR EMBL; AF015825; AAC46335.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13096.2; -; Genomic_DNA.
DR PIR; D69853; D69853.
DR RefSeq; NP_389121.2; NC_000964.3.
DR RefSeq; WP_003245409.1; NZ_JNCM01000035.1.
DR AlphaFoldDB; O34673; -.
DR SMR; O34673; -.
DR STRING; 224308.BSU12390; -.
DR PaxDb; 224308-BSU12390; -.
DR EnsemblBacteria; CAB13096; CAB13096; BSU_12390.
DR GeneID; 939837; -.
DR KEGG; bsu:BSU12390; -.
DR PATRIC; fig|224308.179.peg.1340; -.
DR eggNOG; COG2721; Bacteria.
DR InParanoid; O34673; -.
DR OrthoDB; 9804574at2; -.
DR PhylomeDB; O34673; -.
DR BioCyc; BSUB:BSU12390-MONOMER; -.
DR UniPathway; UPA00246; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0008789; F:altronate dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0019698; P:D-galacturonate catabolic process; IBA:GO_Central.
DR CDD; cd11613; SAF_AH_GD; 1.
DR Gene3D; 2.30.130.110; -; 1.
DR InterPro; IPR048332; GD_AH_C.
DR InterPro; IPR007392; GD_AH_second.
DR InterPro; IPR013974; SAF.
DR InterPro; IPR044144; UxaA/GarD_SAF.
DR PANTHER; PTHR30536:SF5; ALTRONATE DEHYDRATASE; 1.
DR PANTHER; PTHR30536; ALTRONATE/GALACTARATE DEHYDRATASE; 1.
DR Pfam; PF20629; GD_AH_C; 1.
DR Pfam; PF04295; GD_AH_second; 1.
DR Pfam; PF08666; SAF; 1.
DR SMART; SM00858; SAF; 1.
PE 2: Evidence at transcript level;
KW Iron; Lyase; Manganese; Reference proteome.
FT CHAIN 1..497
FT /note="Altronate dehydratase"
FT /id="PRO_0000172282"
FT CONFLICT 19
FT /note="D -> Y (in Ref. 1; AAC46335)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 497 AA; 54872 MW; 5B58AD93B572C3E9 CRC64;
MKSFIKIHKQ DNVLLALRDI QKGERLHAYG VSIEVKDDIK RGHKIALQSI KENDSIVKYG
FPIGHASQDI SIGEHIHVHN TKTNLSDIQL YSYTPRFDEN PYSNENRTFK GFRRENGDAG
VRNELWIVPT VGCVNGIAEK MLQRFVRETG DIAPFDNVLV LKHQYGCSQL GDDHENTKQI
LLNAIRHPNA GGVLVLGLGC ENNELARMKE ALQDVNLKRV KFLESQSVTD EMEAGVALLK
EIHEAAKGDK REDIPLSELK IGLKCGGSDG FSGITANPLL GRFSDYLIAQ GGSTVLTEVP
EMFGAETILM QRAANEEVFH KIVDLINDFK QYFIKHDQPV YENPSPGNKA GGISTLEDKS
LGCTQKAGIS PVTDVLKYGE VLKTKGLTLL SAPGNDLIAS SALAAAGCQI VLFTTGRGTP
FGTFVPTVKV ATNTELYEAK PHWIDFNAGL LAEDDVHEEY VLREFIHYMI EVASGQLVNH
EKNDFKELAI FKSGVTL
//
