ID V2YGE1_9FIRM Unreviewed; 659 AA.
AC V2YGE1;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 24-JAN-2024, entry version 43.
DE RecName: Full=Radical SAM core domain-containing protein {ECO:0000259|PROSITE:PS51918};
GN ORFNames=N510_001647 {ECO:0000313|EMBL:USF26715.1}, N510_01479
GN {ECO:0000313|EMBL:ESL14271.1};
OS Firmicutes bacterium ASF500.
OC Bacteria; Bacillota.
OX NCBI_TaxID=1378168 {ECO:0000313|EMBL:ESL14271.1};
RN [1] {ECO:0000313|EMBL:ESL14271.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ASF500 {ECO:0000313|EMBL:ESL14271.1};
RX PubMed=24723722;
RA Wannemuehler M.J., Overstreet A.M., Ward D.V., Phillips G.J.;
RT "Draft genome sequences of the altered schaedler flora, a defined bacterial
RT community from gnotobiotic mice.";
RL Genome Announc. 2:e00287-e00214(2014).
RN [2] {ECO:0000313|EMBL:USF26715.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ASF500 {ECO:0000313|EMBL:USF26715.1};
RA Proctor A., Parvinroo S., Richie T., Jia X., Lee S.T.M., Karp P.D.,
RA Paley S., Kostic A.D., Pierre J.F., Wannemuehler M.J., Phillips G.J.;
RT "Resources to Facilitate Use of the Altered Schaedler Flora (ASF) Mouse
RT Model to Study Microbiome Function.";
RL Submitted (MAY-2022) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_01251};
CC Note=Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3
CC cysteines and an exchangeable S-adenosyl-L-methionine.
CC {ECO:0000256|HAMAP-Rule:MF_01251};
CC -!- SIMILARITY: Belongs to the UPF0313 family. {ECO:0000256|HAMAP-
CC Rule:MF_01251}.
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DR EMBL; AYJP01000006; ESL14271.1; -; Genomic_DNA.
DR EMBL; CP097573; USF26715.1; -; Genomic_DNA.
DR AlphaFoldDB; V2YGE1; -.
DR STRING; 1378168.N510_01479; -.
DR PATRIC; fig|1378168.3.peg.1547; -.
DR eggNOG; COG1032; Bacteria.
DR HOGENOM; CLU_018288_2_0_9; -.
DR OrthoDB; 9803479at2; -.
DR Proteomes; UP000017395; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR HAMAP; MF_01251; UPF0313; 1.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR InterPro; IPR022946; UPF0313.
DR InterPro; IPR024560; UPF0313_C.
DR InterPro; IPR013704; UPF0313_N.
DR NCBIfam; TIGR03904; SAM_YgiQ; 1.
DR PANTHER; PTHR32331; UPF0313 PROTEIN YGIQ; 1.
DR PANTHER; PTHR32331:SF0; UPF0313 PROTEIN YGIQ; 1.
DR Pfam; PF11842; DUF3362; 1.
DR Pfam; PF08497; Radical_SAM_N; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SFLD; SFLDG01069; UPF0313; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485, ECO:0000256|HAMAP-Rule:MF_01251};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|HAMAP-Rule:MF_01251};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|HAMAP-
KW Rule:MF_01251};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723, ECO:0000256|HAMAP-
KW Rule:MF_01251}; Reference proteome {ECO:0000313|Proteomes:UP000017395};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691, ECO:0000256|HAMAP-
KW Rule:MF_01251}.
FT DOMAIN 297..570
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
FT REGION 596..659
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 623..645
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 312
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01251"
FT BINDING 316
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01251"
FT BINDING 319
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /ligand_note="4Fe-4S-S-AdoMet"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_01251"
SQ SEQUENCE 659 AA; 74151 MW; 8BE53D3FA4A94D54 CRC64;
MPFDRDLFLP VSREDMARRG WDYYDFLLIT GDAYVDHPSF GTAIIGRILE AEGYRVAVLA
QPDWHSAEAF AALGRPRLGV LIGAGNIDSM VAHYTAAKKR RHDDAYSPGR KTGLRPDHAT
VVYSNRVREA FGDIPIVIGG LEASLRRFAH YDYWEDKVRR SVLFDAQADI LTYGMGEYAT
LEIAARLSSG TPVSEITDVR GTCVAGKYPG LCAYPRVEVP SFEEVSASKE AYARANMVEY
QEHDAVSGKA VIQRHGDRSL IVNPPAFPLT TAELDRVAEL PYVREPHPMY DDMGGVPAIE
EVRFSVTHNR GCFGACNFCS LAFHQGRTIS CRSHDSVIRE VKALTEHPGF KGYIHDVGGP
SATFRRPSCQ KQLKSGMCRN RACLAPEPCP NLDADHTDYM MLLRKLREIP KVKKVFIRSG
IRFDYLMKDP SGEFFTDLVQ HHISGQLKVA PEHCVAHTLD YMGKPHIQVY EKFREKYFKL
NRRYGKDQYL VPYLISSHPG CTLEDAVQLA EWLNEQGHMP EQVQDFYPTP GTLATCMWYT
GIDPRTMKPV FVPKTPHDKA LQRALMQWRK PQNRKLVLEA LHKTGREDLI GYGKHCLVRP
DRGGPPVGRG DPGAPSSKNR PAKGKKPRAD TRTRVEKKES SARPAKKAGW AKAKPKKKR
//
