ID   V4M296_EUTSA            Unreviewed;       789 AA.
AC   V4M296;
DT   22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT   22-JAN-2014, sequence version 1.
DT   27-MAR-2024, entry version 39.
DE   RecName: Full=polynucleotide adenylyltransferase {ECO:0000256|ARBA:ARBA00012388};
DE            EC=2.7.7.19 {ECO:0000256|ARBA:ARBA00012388};
GN   ORFNames=EUTSA_v10001908mg {ECO:0000313|EMBL:ESQ50274.1};
OS   Eutrema salsugineum (Saltwater cress) (Sisymbrium salsugineum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Eutremeae; Eutrema.
OX   NCBI_TaxID=72664 {ECO:0000313|EMBL:ESQ50274.1, ECO:0000313|Proteomes:UP000030689};
RN   [1] {ECO:0000313|EMBL:ESQ50274.1, ECO:0000313|Proteomes:UP000030689}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=23518688; DOI=10.3389/fpls.2013.00046;
RA   Yang R., Jarvis D.E., Chen H., Beilstein M.A., Grimwood J., Jenkins J.,
RA   Shu S., Prochnik S., Xin M., Ma C., Schmutz J., Wing R.A.,
RA   Mitchell-Olds T., Schumaker K.S., Wang X.;
RT   "The Reference Genome of the Halophytic Plant Eutrema salsugineum.";
RL   Front. Plant Sci. 4:46-46(2013).
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|ARBA:ARBA00001946};
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000256|ARBA:ARBA00001936};
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC   -!- SIMILARITY: Belongs to the poly(A) polymerase family.
CC       {ECO:0000256|ARBA:ARBA00010912}.
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DR   EMBL; KI517398; ESQ50274.1; -; Genomic_DNA.
DR   RefSeq; XP_006408821.1; XM_006408758.1.
DR   AlphaFoldDB; V4M296; -.
DR   STRING; 72664.V4M296; -.
DR   EnsemblPlants; ESQ50274; ESQ50274; EUTSA_v10001908mg.
DR   GeneID; 18025642; -.
DR   Gramene; ESQ50274; ESQ50274; EUTSA_v10001908mg.
DR   eggNOG; KOG2245; Eukaryota.
DR   OMA; VVKPCNQ; -.
DR   OrthoDB; 1351913at2759; -.
DR   Proteomes; UP000030689; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:1990817; F:poly(A) RNA polymerase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   GO; GO:0031123; P:RNA 3'-end processing; IEA:InterPro.
DR   CDD; cd05402; NT_PAP_TUTase; 1.
DR   Gene3D; 1.10.1410.10; -; 1.
DR   Gene3D; 3.30.460.10; Beta Polymerase, domain 2; 1.
DR   Gene3D; 3.30.70.590; Poly(A) polymerase predicted RNA binding domain; 1.
DR   InterPro; IPR043519; NT_sf.
DR   InterPro; IPR011068; NuclTrfase_I-like_C.
DR   InterPro; IPR007012; PolA_pol_cen_dom.
DR   InterPro; IPR048840; PolA_pol_NTPase.
DR   InterPro; IPR007010; PolA_pol_RNA-bd_dom.
DR   PANTHER; PTHR10682:SF31; NUCLEAR POLY(A) POLYMERASE 2; 1.
DR   PANTHER; PTHR10682; POLY A POLYMERASE; 1.
DR   Pfam; PF04928; PAP_central; 1.
DR   Pfam; PF20750; PAP_NTPase; 1.
DR   Pfam; PF04926; PAP_RNA-bind; 2.
DR   SUPFAM; SSF81301; Nucleotidyltransferase; 1.
DR   SUPFAM; SSF55003; PAP/Archaeal CCA-adding enzyme, C-terminal domain; 1.
DR   SUPFAM; SSF81631; PAP/OAS1 substrate-binding domain; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   mRNA processing {ECO:0000256|ARBA:ARBA00022664};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW   Reference proteome {ECO:0000313|Proteomes:UP000030689};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT   DOMAIN          19..213
FT                   /note="Poly(A) polymerase nucleotidyltransferase"
FT                   /evidence="ECO:0000259|Pfam:PF20750"
FT   DOMAIN          218..363
FT                   /note="Poly(A) polymerase central"
FT                   /evidence="ECO:0000259|Pfam:PF04928"
FT   DOMAIN          366..424
FT                   /note="Poly(A) polymerase RNA-binding"
FT                   /evidence="ECO:0000259|Pfam:PF04926"
FT   DOMAIN          424..499
FT                   /note="Poly(A) polymerase RNA-binding"
FT                   /evidence="ECO:0000259|Pfam:PF04926"
FT   REGION          489..577
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        523..549
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   789 AA;  89756 MW;  48A2B6C8C80E9155 CRC64;
     MVGTQRSEEP PVKALKSYGI TKPLSVTGPS AADLKRNLEL EKFLVDEGLY ESNEETMRRE
     EVLGRMDQIV KHWVKHLTRQ RGYTDQMVED ANAVIFTFGS YRLGVHGPGA DIDTLCVGPS
     YVNREEDFFI ILRDILAEME EVTELQPVTD AHVPVMKFKF QGISIDLLYA SISLLVVPQD
     LDISNSSVLC DVDEQTVRSL NGCRVADQIL KLVPNFEHFR TTLRCMKYWA KKRGVYSNVT
     GFLGGVNWAL LVARLCQLYP NAIPSMLVSR FFRVYTQWRW PNPVMLCAIE ENELGIPVWD
     PRKNHRDRYH LMPIITPAYP CMNSSYNVSQ STLRVMTEQF QYGNKICQEI ELNKQHWSSL
     FEQYMFFEAY KNYLQVDVLA VDAEDLLAWK GWVESRFRQL TLKIERDTNG MLMCHPQPNE
     YVDTSKQFRH CAFFMGLQRA EGVGGQECQQ FDIRGTVDEF RQEVNMYMFW RPGMDVYVSH
     VRRRQLPPFV FPNGYKRPRQ SRHQNQQSRE PGEEGVGSVS AYVERHAKRK NDNEMIDTRP
     EKPEKRASLS PHSLDAVYPD SSAITTGETP PIGIESGPRA ECLVTTDLDC TTPNVEVEAE
     RFIRKSTEII EVSQREHNSG NEQTLEVDSR ALVQGCHDLA EPVEKHVRPD PSAVVACEGG
     HNEDEGRNMC SEAINDDTPH LPRRLNLKEN VDEVERESRS GKIADGALRN GYCGQNLDHE
     GFFTGASLDP SVEHKNLHSD GLFKSGLSEE LQSNPLLSGM GRPEEGARSE SLSIDEACIL
     QPVTILCQS
//