ID   V5DEM3_TRYCR            Unreviewed;       765 AA.
AC   V5DEM3;
DT   22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT   22-JAN-2014, sequence version 1.
DT   24-JAN-2024, entry version 34.
DE   RecName: Full=methionine--tRNA ligase {ECO:0000256|ARBA:ARBA00012838};
DE            EC=6.1.1.10 {ECO:0000256|ARBA:ARBA00012838};
GN   ORFNames=TCDM_05606 {ECO:0000313|EMBL:ESS65891.1};
OS   Trypanosoma cruzi Dm28c.
OC   Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina;
OC   Trypanosomatida; Trypanosomatidae; Trypanosoma; Schizotrypanum.
OX   NCBI_TaxID=1416333 {ECO:0000313|EMBL:ESS65891.1, ECO:0000313|Proteomes:UP000017861};
RN   [1] {ECO:0000313|EMBL:ESS65891.1, ECO:0000313|Proteomes:UP000017861}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Dm28c {ECO:0000313|EMBL:ESS65891.1,
RC   ECO:0000313|Proteomes:UP000017861};
RX   PubMed=24482508;
RA   Grisard E.C., Teixeira S.M., de Almeida L.G., Stoco P.H., Gerber A.L.,
RA   Talavera-Lopez C., Lima O.C., Andersson B., de Vasconcelos A.T.;
RT   "Trypanosoma cruzi Clone Dm28c Draft Genome Sequence.";
RL   Genome Announc. 2:e01114-13(2014).
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000256|RuleBase:RU363039}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:ESS65891.1}.
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DR   EMBL; AYLP01000055; ESS65891.1; -; Genomic_DNA.
DR   AlphaFoldDB; V5DEM3; -.
DR   EnsemblProtists; ESS65891; ESS65891; TCDM_05606.
DR   VEuPathDB; TriTrypDB:TCDM_05606; -.
DR   Proteomes; UP000017861; Unassembled WGS sequence.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004825; F:methionine-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006431; P:methionyl-tRNA aminoacylation; IEA:InterPro.
DR   CDD; cd07957; Anticodon_Ia_Met; 1.
DR   CDD; cd00814; MetRS_core; 1.
DR   Gene3D; 2.170.220.10; -; 1.
DR   Gene3D; 3.40.50.620; HUPs; 1.
DR   InterPro; IPR041872; Anticodon_Met.
DR   InterPro; IPR014758; Met-tRNA_synth.
DR   InterPro; IPR023457; Met-tRNA_synth_2.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR033911; MetRS_core.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   NCBIfam; TIGR00398; metG; 1.
DR   PANTHER; PTHR43326:SF1; METHIONINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR43326; METHIONYL-TRNA SYNTHETASE; 1.
DR   Pfam; PF19303; Anticodon_3; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR01041; TRNASYNTHMET.
DR   SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR   SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW   ECO:0000256|RuleBase:RU363039};
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363039};
KW   Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363039};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW   ECO:0000256|RuleBase:RU363039};
KW   Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW   ECO:0000256|RuleBase:RU363039};
KW   Reference proteome {ECO:0000313|Proteomes:UP000017861}.
FT   DOMAIN          235..609
FT                   /note="Methionyl/Leucyl tRNA synthetase"
FT                   /evidence="ECO:0000259|Pfam:PF09334"
FT   DOMAIN          622..743
FT                   /note="Methionyl-tRNA synthetase anticodon-binding"
FT                   /evidence="ECO:0000259|Pfam:PF19303"
SQ   SEQUENCE   765 AA;  86094 MW;  AAE2FD0B816995F4 CRC64;
     MSLKLFSEKA NTQALKALLC CHHVRRPVEL TLSGAYTSPV LHHPAFKQPI FSANEMVRLI
     LHDKWRDDSA GGSGGKKTGC YDGDWASVSL EEEAWLEWEA TTFTCAVHPL YTQRRVTPEA
     AGAFEYLDNK IHENDCRGPC LAPVGEKGTS TKSPSFLVDC IIWCAVLPAL CEGGLLGEKE
     REKVPHLLKW FESFQSAREA HIAGAVESLA VQEFADFLRA PRVYKLSPKL SKVFFVTTPI
     YYVNAAPHIG HVYSTLIADV IGRYHTIKGE RVFAMSGTDE HGQKVAEAAR QKGMNPYDFT
     FQVSREFIDC FEQMGYHMDY FIRTTNESHK KVVHELWSKL EAKGDIYLGR YEGWYSVSDE
     SFLTAQNVTD GVDKDGKPCK VSLESGHVVT WLAEDNYMFR LSAFRERLLE WYNNNPGCIV
     PEFRRREVIR TVEKGLNDLS VSRTREAVQD WAIPVPGNPN HCIYVWLDAL SNYYTGSRLR
     LGNSGEELEL VEDHRVLERF PADIHVIGKD ILKFHAIYWP AFLISAGLPL PKTIVAHGWW
     TKDRKKISKS LGNVFDPVEK AREFGFDALK YFLLRETGFS DDGDYSDRNM IARLNGELAD
     TLGNLVMRCT SAKINVHREW PTPDAYNAKD EALIQLIKEL PGVVDHFFLI PDVQKALAAV
     FDVLRAINSY VTENAPWRLV KTDPDRLRTV LYITMEGTRL ATLMLSPVLS QKAPVIFDML
     GVPEANRVGV ENFGFGVVPS GTPIGDAVEG KALFPKISLD KAQSA
//