ID V5NK73_HELPX Unreviewed; 750 AA.
AC V5NK73;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE RecName: Full=Carbamoyltransferase {ECO:0000256|PIRNR:PIRNR006256};
DE EC=6.2.-.- {ECO:0000256|PIRNR:PIRNR006256};
GN ORFNames=U064_0048 {ECO:0000313|EMBL:AHA89000.1};
OS Helicobacter pylori BM012S.
OC Bacteria; Campylobacterota; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=1407463 {ECO:0000313|EMBL:AHA89000.1, ECO:0000313|Proteomes:UP000018543};
RN [1] {ECO:0000313|EMBL:AHA89000.1, ECO:0000313|Proteomes:UP000018543}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BM012S {ECO:0000313|EMBL:AHA89000.1,
RC ECO:0000313|Proteomes:UP000018543};
RX DOI=10.1371/journal.pone.0082187;
RA Linz B., Windsor H.M., Gajewski J.P., Hake C.M., Drautz D.I.,
RA Schuster S.C., Marshall B.J.;
RT "Helicobacter pylori genomic microevolution during naturally occurring
RT transmission between adults.";
RL PLoS ONE 8:E82187-E82187(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + C-terminal L-cysteinyl-[HypE protein] + carbamoyl
CC phosphate + H2O = AMP + C-terminal S-carboxamide-L-cysteinyl-[HypE
CC protein] + diphosphate + H(+) + phosphate; Xref=Rhea:RHEA:55636,
CC Rhea:RHEA-COMP:14247, Rhea:RHEA-COMP:14392, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58228, ChEBI:CHEBI:76913,
CC ChEBI:CHEBI:139126, ChEBI:CHEBI:456215;
CC Evidence={ECO:0000256|ARBA:ARBA00001186};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an acyl phosphate + H2O = a carboxylate + H(+) + phosphate;
CC Xref=Rhea:RHEA:14965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29067, ChEBI:CHEBI:43474, ChEBI:CHEBI:59918; EC=3.6.1.7;
CC Evidence={ECO:0000256|PROSITE-ProRule:PRU00520};
CC -!- PATHWAY: Protein modification; [NiFe] hydrogenase maturation.
CC {ECO:0000256|ARBA:ARBA00004711}.
CC -!- SIMILARITY: Belongs to the carbamoyltransferase HypF family.
CC {ECO:0000256|ARBA:ARBA00008097, ECO:0000256|PIRNR:PIRNR006256}.
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DR EMBL; CP006889; AHA89000.1; -; Genomic_DNA.
DR RefSeq; WP_023591502.1; NC_022911.1.
DR AlphaFoldDB; V5NK73; -.
DR KEGG; hez:U064_0048; -.
DR PATRIC; fig|1407463.3.peg.46; -.
DR HOGENOM; CLU_009164_0_0_7; -.
DR UniPathway; UPA00335; -.
DR Proteomes; UP000018543; Chromosome.
DR GO; GO:0003998; F:acylphosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0016743; F:carboxyl- or carbamoyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003725; F:double-stranded RNA binding; IEA:InterPro.
DR GO; GO:0016874; F:ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR Gene3D; 3.30.110.120; -; 1.
DR Gene3D; 3.30.420.360; -; 1.
DR Gene3D; 3.30.420.40; -; 1.
DR Gene3D; 3.90.870.50; -; 1.
DR InterPro; IPR001792; Acylphosphatase-like_dom.
DR InterPro; IPR036046; Acylphosphatase-like_dom_sf.
DR InterPro; IPR017968; Acylphosphatase_CS.
DR InterPro; IPR004421; Carbamoyltransferase_HypF.
DR InterPro; IPR017945; DHBP_synth_RibB-like_a/b_dom.
DR InterPro; IPR041440; HypF_C.
DR InterPro; IPR006070; Sua5-like_dom.
DR InterPro; IPR011125; Znf_HypF.
DR NCBIfam; TIGR00143; hypF; 1.
DR PANTHER; PTHR42959; CARBAMOYLTRANSFERASE; 1.
DR PANTHER; PTHR42959:SF1; CARBAMOYLTRANSFERASE HYPF; 1.
DR Pfam; PF00708; Acylphosphatase; 1.
DR Pfam; PF17788; HypF_C; 1.
DR Pfam; PF01300; Sua5_yciO_yrdC; 1.
DR Pfam; PF07503; zf-HYPF; 2.
DR PIRSF; PIRSF006256; CMPcnvr_hdrg_mat; 1.
DR SUPFAM; SSF54975; Acylphosphatase/BLUF domain-like; 1.
DR SUPFAM; SSF55821; YrdC/RibB; 1.
DR PROSITE; PS00150; ACYLPHOSPHATASE_1; 1.
DR PROSITE; PS51160; ACYLPHOSPHATASE_3; 1.
DR PROSITE; PS51163; YRDC; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|PROSITE-ProRule:PRU00520};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771}.
FT DOMAIN 1..86
FT /note="Acylphosphatase-like"
FT /evidence="ECO:0000259|PROSITE:PS51160"
FT DOMAIN 192..376
FT /note="YrdC-like"
FT /evidence="ECO:0000259|PROSITE:PS51163"
FT ACT_SITE 16
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00520"
FT ACT_SITE 34
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00520"
SQ SEQUENCE 750 AA; 84754 MW; B5D15481DD04A6B4 CRC64;
MNKITLFGVV QGVGMRPFIY TLAQKLELVG FVRNAQVALE IVLPAHQTES FLNALKKESP
PLALVEKIII SPYDKALHFN DFRILESKNH PLNLLSQIPK DLGVCEDCLR EIRDKNSPYF
YYAFNSCAKC GARYSLLNAM PYDRENSALK PFKLCEFCAS VYQDPTNKRF HIQGISCKKC
GIALNYKRFK NDDALLECAK DIQKGKIIAL KGLGGFALLC DGRNFQTIER LRLLKNRPLK
PFALMFKDLN TAKQHAFLNA SEGESLISAS APILLARKKP DTPLAPNIAK NSPFYGVILP
YTPLHALLLD LLDFPIVFTS ANFSSLPLAS DEAEIDSLSF IFDFKLTHNR AIIHRIDDSI
AQLVDNAIRP MRLARGFAPL YLALPKRSNG SPKKILALGA QQKGHFSLLD SETSILLLSP
FCGDLSVLEN EKHFKETLNF FLKTYDFKPT ILACDKHQNY TTTKMACGFN TPLLQVQHHH
AHFLASVLDA LLQDPHLNHP FIGIVWDGSG AYENKVYGAE CFVGDFERIE EVARFEEFWL
LGGQKAIKEP KRLVLEISLK HQLNKLLKRV QKHFKEDELE IFQQMHDRKI QSIATNSIGR
LFDIIAFSLD LTGTISFEAE SGQVLENLAL QSDEIAFYPF TIKNSVVCLK EFYQAFEKDL
GVLEPERIAK KFFNSLVEII TALIAPFKEH VVVCSGGVFC NQLLCEQLAK RLRGLKRQYF
FHKHFPPNDS SIPIGQALMA YFNPTIIKKG
//
