ID V5TJK2_HALHI Unreviewed; 546 AA.
AC V5TJK2;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE SubName: Full=Branched-chain alpha-keto acid dehydrogenase subunit E2 {ECO:0000313|EMBL:AHB64674.1};
GN ORFNames=HISP_01160 {ECO:0000313|EMBL:AHB64674.1};
OS Haloarcula hispanica N601.
OC Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC Haloarculaceae; Haloarcula.
OX NCBI_TaxID=1417673 {ECO:0000313|Proteomes:UP000018572};
RN [1] {ECO:0000313|EMBL:AHB64674.1, ECO:0000313|Proteomes:UP000018572}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=N601 {ECO:0000313|EMBL:AHB64674.1,
RC ECO:0000313|Proteomes:UP000018572};
RX PubMed=24625874;
RA Ding J.Y., Chiang P.W., Hong M.J., Dyall-Smith M., Tang S.L.;
RT "Complete Genome Sequence of the Extremely Halophilic Archaeon Haloarcula
RT hispanica Strain N601.";
RL Genome Announc. 2:e00178-14(2014).
CC -!- COFACTOR:
CC Name=(R)-lipoate; Xref=ChEBI:CHEBI:83088;
CC Evidence={ECO:0000256|ARBA:ARBA00001938};
CC -!- SIMILARITY: Belongs to the 2-oxoacid dehydrogenase family.
CC {ECO:0000256|ARBA:ARBA00007317}.
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DR EMBL; CP006884; AHB64674.1; -; Genomic_DNA.
DR RefSeq; WP_014039227.1; NC_023013.1.
DR AlphaFoldDB; V5TJK2; -.
DR GeneID; 23804917; -.
DR KEGG; hhn:HISP_01160; -.
DR HOGENOM; CLU_016733_10_0_2; -.
DR Proteomes; UP000018572; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0016746; F:acyltransferase activity; IEA:InterPro.
DR CDD; cd06849; lipoyl_domain; 1.
DR Gene3D; 2.40.50.100; -; 1.
DR Gene3D; 3.30.559.10; Chloramphenicol acetyltransferase-like domain; 1.
DR Gene3D; 4.10.320.10; E3-binding domain; 1.
DR InterPro; IPR003016; 2-oxoA_DH_lipoyl-BS.
DR InterPro; IPR001078; 2-oxoacid_DH_actylTfrase.
DR InterPro; IPR000089; Biotin_lipoyl.
DR InterPro; IPR023213; CAT-like_dom_sf.
DR InterPro; IPR036625; E3-bd_dom_sf.
DR InterPro; IPR004167; PSBD.
DR InterPro; IPR011053; Single_hybrid_motif.
DR PANTHER; PTHR43178; DIHYDROLIPOAMIDE ACETYLTRANSFERASE COMPONENT OF PYRUVATE DEHYDROGENASE COMPLEX; 1.
DR PANTHER; PTHR43178:SF5; LIPOAMIDE ACYLTRANSFERASE COMPONENT OF BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX, MITOCHONDRIAL; 1.
DR Pfam; PF00198; 2-oxoacid_dh; 1.
DR Pfam; PF00364; Biotin_lipoyl; 1.
DR Pfam; PF02817; E3_binding; 1.
DR SUPFAM; SSF52777; CoA-dependent acyltransferases; 1.
DR SUPFAM; SSF47005; Peripheral subunit-binding domain of 2-oxo acid dehydrogenase complex; 1.
DR SUPFAM; SSF51230; Single hybrid motif; 1.
DR PROSITE; PS50968; BIOTINYL_LIPOYL; 1.
DR PROSITE; PS00189; LIPOYL; 1.
DR PROSITE; PS51826; PSBD; 1.
PE 3: Inferred from homology;
KW Lipoyl {ECO:0000256|ARBA:ARBA00022823}.
FT DOMAIN 1..76
FT /note="Lipoyl-binding"
FT /evidence="ECO:0000259|PROSITE:PS50968"
FT DOMAIN 131..168
FT /note="Peripheral subunit-binding (PSBD)"
FT /evidence="ECO:0000259|PROSITE:PS51826"
FT REGION 92..129
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 147..212
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 291..322
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 113..128
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 175..204
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 546 AA; 56519 MW; 4199BCF18D877655 CRC64;
MFEFNLPDLG EGVAEGEVLT WHVSPGDAVT EDQVLAEVET DKAAVDVPSP VDGVVQELHA
EVGEMVQTGE VLITIDEAGD AEAADAAAGS AADAESAEAT TEAVADDTAS EATAADASDQ
SDASTADGRV FASPSVRRLA REKGVDIAAV DGSGPGGRVT EGDVEAATAS ADETADSDDG
PTSVVSEASD DGDGPTTAVS QVDDADTDDG NAVKSAVRSV SGDGQAAANR DQTLATPATR
KAARELDVNI DAVPTDQTRD GQPYVDESAV RTYAEAQQAA QAADAEAVSA EGGAAGTAAE
AGGAGAETTA VETGDGERRE PYRGVRRSIG EQMARSRREV PHATHHDQVD VSGLVEAHER
LQPLAEERDV TLTYTPFVVK CVAAALDQHP VLNTALDTEN EEIVYRDVHN IGVAAATDHG
LVVPVVDDVD GKCLVELADE INDLVGRARE RDIERSEMQG GTFTVTNFGA IGGEYASPII
NVPETAILGI GALKERPVAE DGEVVAKPTL PLSLAIDHRV IDGADAARFV NTLKEYLTDP
TRLLLE
//
