ID V6KFH6_STRNV Unreviewed; 2178 AA.
AC V6KFH6;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE RecName: Full=Ketosynthase family 3 (KS3) domain-containing protein {ECO:0000259|PROSITE:PS52004};
GN ORFNames=M877_08380 {ECO:0000313|EMBL:EST30925.1};
OS Streptomyces niveus NCIMB 11891.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1352941 {ECO:0000313|EMBL:EST30925.1, ECO:0000313|Proteomes:UP000017971};
RN [1] {ECO:0000313|EMBL:EST30925.1, ECO:0000313|Proteomes:UP000017971}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCIMB 11891 {ECO:0000313|EMBL:EST30925.1,
RC ECO:0000313|Proteomes:UP000017971};
RX PubMed=24407644;
RA Flinspach K., Ruckert C., Kalinowski J., Heide L., Apel A.K.;
RT "Draft Genome Sequence of Streptomyces niveus NCIMB 11891, Producer of the
RT Aminocoumarin Antibiotic Novobiocin.";
RL Genome Announc. 2:e01146-e01113(2014).
CC -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005194}.
CC -!- SIMILARITY: Belongs to the thioester dehydratase family. FabA
CC subfamily. {ECO:0000256|ARBA:ARBA00006714}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EST30925.1}.
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DR EMBL; AWQW01000058; EST30925.1; -; Genomic_DNA.
DR RefSeq; WP_023537968.1; NZ_CM002280.1.
DR STRING; 193462.BBN63_02825; -.
DR PATRIC; fig|1352941.4.peg.1717; -.
DR eggNOG; COG0764; Bacteria.
DR eggNOG; COG3321; Bacteria.
DR HOGENOM; CLU_000022_30_0_11; -.
DR OrthoDB; 9778690at2; -.
DR UniPathway; UPA00094; -.
DR Proteomes; UP000017971; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0008693; F:(3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity; IEA:InterPro.
DR GO; GO:0008659; F:(3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0016747; F:acyltransferase activity, transferring groups other than amino-acyl groups; IEA:UniProt.
DR GO; GO:0034017; F:trans-2-decenoyl-acyl-carrier-protein isomerase activity; IEA:UniProtKB-EC.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd01287; FabA; 2.
DR CDD; cd00833; PKS; 1.
DR Gene3D; 3.40.47.10; -; 2.
DR Gene3D; 3.10.129.10; Hotdog Thioesterase; 4.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 1.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR010083; FabA.
DR InterPro; IPR013114; FabA_FabZ.
DR InterPro; IPR029069; HotDog_dom_sf.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43074; OMEGA-3 POLYUNSATURATED FATTY ACID SYNTHASE PFAB-RELATED; 1.
DR PANTHER; PTHR43074:SF1; PKS_AT DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF00698; Acyl_transf_1; 1.
DR Pfam; PF07977; FabA; 2.
DR Pfam; PF16197; KAsynt_C_assoc; 1.
DR Pfam; PF00109; ketoacyl-synt; 2.
DR Pfam; PF02801; Ketoacyl-synt_C; 1.
DR SMART; SM00827; PKS_AT; 1.
DR SMART; SM00825; PKS_KS; 1.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 1.
DR SUPFAM; SSF54637; Thioesterase/thiol ester dehydrase-isomerase; 4.
DR SUPFAM; SSF53901; Thiolase-like; 3.
DR PROSITE; PS52004; KS3_2; 1.
PE 3: Inferred from homology;
KW Antibiotic biosynthesis {ECO:0000256|ARBA:ARBA00023194};
KW Fatty acid biosynthesis {ECO:0000256|ARBA:ARBA00023160};
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00023160};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023160};
KW NADP {ECO:0000256|ARBA:ARBA00022857};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT DOMAIN 3..438
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT REGION 1316..1353
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1320..1349
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2178 AA; 230780 MW; 53BB2B143D2A9449 CRC64;
MEFEPIAIVG RGCVLPDALD PDTFWDNIAA GRTSLSAAPD GDRWRLPRRL AMGTVGDHLD
RTWTDAGGYV RGFESVFDPG GFRIAPERIL SLDPLFHWVM YGVRQALTEA GREGPLPRAG
LVLGNLSYPT PTGAVYAEHV WLSAQQPALR DALLTGERRR RPDARNRFSS GLPARLAARA
LGLGAGAWSL DAACASSLYA VKLACDRLHD GTADLMVAGA VSRADPLYLH VGFCGLSATS
RTGRSRPFHR DADGLVHGEG AGFVALMRLS DARAAGLPVL GVIRGVGLSN DGRGAGLISP
AEEGQVRAMR LAYEAAGVAP GTVSLVECHA TGTPVGDAVE ARGMAQVFGA GADVPIGSVK
SNVGHLLASA GVAGLLKVLG ALRTGVRPAT LGAELPLDAL ADTPLRVLTA PEPWSGPRRA
AVSAFGFGGT NAHLVVDHPD DGLVSAVPRP SRPVAAVTAP IGVREAPGGR TPVAIVALGA
RVGDGTCTED FRRAVLGGER RGPAPGIAVE LTDLCFPPVG LERTVRHQLL VLEAAREAVR
SVSLPRERTM VVVGMGVDPE VARAGARWRV PHWLEQAGLG ATPESADLAR DAFVPPMTAE
GVVGTLPNLV ANRISTQLDL AGPGFAVSAE EASGLVALEL AARAVRSGEV DAALVGATDL
SCEAVHRSAL RDLGHEHEPG DAAVVLVLKS LETARRDGDT VVALLDDVTA DEPAAVPDLV
VGDVPDSAYD PASAFGRAHA AYGLVCVAVA ALSLQHRALP RPGLPADTAA VVRTAKVAVT
PLEGPTASVR LRAGDTRPWL RGPALRLYVF SGADRREVLA ALESGTRSDT GPARLVLVAD
DDALPGRKEA ARRWLADGGA RPADVLYRDR PVTGRTAFVY TNGSAAYPGM GHELMLALPS
LGEAVRARHG AIGARLRSGA GRGVLDQIWG AAELAVHHTV FTRELLGLRP DAALGYSSGE
SAALVALGAW PDASGLYDAT RDSGLFTTEL TGELRAVRRH WQRLGVEGRR WSSYLVSAPL
DAVRAELAVE VAVHLMAVNA PCVCVIGGES GACAAVVARI GADRAIELDY DMAAHAPELA
EVRDVWRAVH RRPTVDVPGV RFYSGATGQS YRPTAEGAAD ALTAQGLNTI DFAATVEQAW
ADGVRVFVEH GPRKLCTGWI KRVLGDREHV AVALDAPGDT GLRQACLAVA ELVAAGVPVQ
AGALFARLAE AATDLPRPGR TVTVPVLAPP CLPPLAPAVV TLPRAPELVP VPDTPGGAVV
SGGPGSDTPG VLATVLRQSR RVAALHQEIT AGHTEAHQRF LQLSALAVTA LRSTAPAAPA
APVRPTPRHT PRPAPLPAAP APEAAPAQLP KPGPKFDRAQ LEYLASGKIS TLFGPRFAEQ
DAYPVQTRMP GPPMLFADRV TGIDAVPAAL AAPGPVRTDG RIWTETDVRA DSWYLDATGR
MPAGLLIEAG QADLLLLSWL GVDLINRGER AYRLLGCEVT YHGRPPEAGD VLRYEIYIDG
HAEHEDVRLA FFHYDCYVDG ELRLSIREGQ AGFFTPGELA GSGGVRWDPT ERPPGDDLPL
DPPAVRCGRT RFDADRVRSL TRGRPADCFG PGWEATAAHV RSPRLDDGRL RLIDEVTAFD
PAGGPWGRGY LRAETALSTD DWFFEGHFRN DPCMPGTLML QGGLQAMAFY LTAMGFTVNR
DGWRFEPVGD HPCKAMCRGQ ATPDNQRVVY EVFVRGVSAG PEPTLYADVL GSVDGARAFL
GRDVALRLVP DWPLSHWRHS GPPAVQGSGT PVPLPLLGGL SGHEEKRPVA TAADGLPFGY
ASLLACAWGR PSEAFGTAYE PFDSARKVAR LPGPPYHFMS RIVSVDGPQG GMREGSGVVA
EYEVPASAWY FEQSGGRTMP FAVLMEIALQ PCGWLASYAG SALTSDTDLL FRNLDGAGTV
TGEVTPATGT VRTHAELTRI SRSGDMIIES FDVRCTADGV PVFELSTVFG YFPPSAFADQ
QGLTVSPEDR ARLYEPCDRV TDLTTRPARY RAGEPRLPGP MLLMLDRITG YWPDGGGAGL
GRLRSEKDVV PDEWFFRAHF FQDPVQPGSL GVEAMCQLLQ YHLLERGAAT GIPHPRFEPV
LPGHETTWTY RGQITPSTRL IRVTMDITES GRDERGPYAL ADATLWGDDT CIYRVRGLGV
RVVSDQWAKG GEKQPVSM
//
