ID V7BFZ5_PHAVU Unreviewed; 1118 AA.
AC V7BFZ5;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE RecName: Full=phosphoenolpyruvate carboxylase {ECO:0000256|ARBA:ARBA00012305};
DE EC=4.1.1.31 {ECO:0000256|ARBA:ARBA00012305};
GN ORFNames=PHAVU_007G101300g {ECO:0000313|EMBL:ESW15778.1};
OS Phaseolus vulgaris (Kidney bean) (French bean).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Phaseolus.
OX NCBI_TaxID=3885 {ECO:0000313|EMBL:ESW15778.1, ECO:0000313|Proteomes:UP000000226};
RN [1] {ECO:0000313|Proteomes:UP000000226}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. G19833 {ECO:0000313|Proteomes:UP000000226};
RX PubMed=24908249; DOI=10.1038/ng.3008;
RA Schmutz J., McClean P.E., Mamidi S., Wu G.A., Cannon S.B., Grimwood J.,
RA Jenkins J., Shu S., Song Q., Chavarro C., Torres-Torres M., Geffroy V.,
RA Moghaddam S.M., Gao D., Abernathy B., Barry K., Blair M., Brick M.A.,
RA Chovatia M., Gepts P., Goodstein D.M., Gonzales M., Hellsten U.,
RA Hyten D.L., Jia G., Kelly J.D., Kudrna D., Lee R., Richard M.M.,
RA Miklas P.N., Osorno J.M., Rodrigues J., Thareau V., Urrea C.A., Wang M.,
RA Yu Y., Zhang M., Wing R.A., Cregan P.B., Rokhsar D.S., Jackson S.A.;
RT "A reference genome for common bean and genome-wide analysis of dual
RT domestications.";
RL Nat. Genet. 46:707-713(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC Evidence={ECO:0000256|ARBA:ARBA00001071};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the PEPCase type 1 family.
CC {ECO:0000256|ARBA:ARBA00008346}.
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DR EMBL; CM002294; ESW15778.1; -; Genomic_DNA.
DR RefSeq; XP_007143784.1; XM_007143722.1.
DR AlphaFoldDB; V7BFZ5; -.
DR STRING; 3885.V7BFZ5; -.
DR EnsemblPlants; ESW15778; ESW15778; PHAVU_007G101300g.
DR GeneID; 18625444; -.
DR Gramene; ESW15778; ESW15778; PHAVU_007G101300g.
DR KEGG; pvu:PHAVU_007G101300g; -.
DR eggNOG; ENOG502QPVS; Eukaryota.
DR OMA; FGWTQSR; -.
DR OrthoDB; 355614at2759; -.
DR PhylomeDB; V7BFZ5; -.
DR Proteomes; UP000000226; Chromosome 7.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-EC.
DR GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR Gene3D; 1.20.1440.90; Phosphoenolpyruvate/pyruvate domain; 2.
DR HAMAP; MF_00595; PEPcase_type1; 1.
DR InterPro; IPR021135; PEP_COase.
DR InterPro; IPR022805; PEP_COase_bac/pln-type.
DR InterPro; IPR018129; PEP_COase_Lys_AS.
DR InterPro; IPR033129; PEPCASE_His_AS.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR PANTHER; PTHR30523; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR PANTHER; PTHR30523:SF6; PHOSPHOENOLPYRUVATE CARBOXYLASE; 1.
DR Pfam; PF00311; PEPcase; 2.
DR PRINTS; PR00150; PEPCARBXLASE.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
DR PROSITE; PS00781; PEPCASE_1; 1.
DR PROSITE; PS00393; PEPCASE_2; 1.
PE 3: Inferred from homology;
KW Carbon dioxide fixation {ECO:0000256|ARBA:ARBA00023300};
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000000226};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 41..63
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT REGION 440..496
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 464..496
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 236
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10111"
FT ACT_SITE 785
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10112"
SQ SEQUENCE 1118 AA; 125049 MW; 7120E2350FFF61B9 CRC64;
MREETVTDID AIQVYKVTIV GVCSIPFTLQ QQPLGSRCAC LPHVLLFPST FTLTPLSLLY
YLLITLLSPS PSSISYLPSF LTMTDITDDV AEEISFQSFD DDCRLLGNLL NDILQREVGT
NLVDKLERIR VLAQSGCNMR QAGILNMAEL LEKQLASELS KMTLEEAFTL ARAFSHYLTL
MGIAETHHRV RKGGNMAQIA KSCDDIFNQL VQGGVPPEKL YDTVCKQEVE IVLTAHPTQI
NRRTLQFKHV RIAHLLDYND RPDLSTEDRE MVIEDLVREI TSIWQTDELR RQKPTPVDEA
RAGFNIVDQS LWKAVPHYLR RVSSALKKHT GKPLPLTCTP IKFGSWMGGD RDGNPNVTAK
VTKDVSLLSR WMAIDLYIRE VDSLRFELSM NQCSDRLSRL AHDILEAKHE NPRENWNQSV
NRSPALPTQL PARAHLPSIA ENGESRHPRL DIPGPDYIQS NHKDGGAALS SNTSKNANPN
IQLSGTSSAN SSASSAGISS SFGQKKLYAE PQTGKSTFQK LLEPMLPQLP GIAPYRIVLG
NVKDKLERSR RRLELLLEDV ACDYDPLEYY ETSDQLLEPL LLCYESLQSC GSGVLADGRL
ADLIRRVATF GMVLMKLDLR QESGRHAEAL DAITEYLDMG TYSEWDEEKK LDFLIKELKG
KRPLVPVSIE VPSDVKEVLD TFRIAAELGS DSLGAYVISM ASNASDVLAV ELLQKDARLA
AIGELGKACP GGTLRVVPLF ETVKDLREAG SVIRKLLSID WYHEHIIKNH NGHQEVMVGY
SDSGKDAGRF TAAWELFKAQ EDVVAACNDY GIKVTLFHGR GGSIGRGGGP TYLAIQSQPP
GSVMGTLRST EQGEMVEAKF GLPQIAVRQL EIYTTAVLLA TLRPPIPPRE EKWRNVMEEI
SNISCQCYRN VVYENPEFLA YFHEATPEAE LGFLNIGSRP ARRKSSRGIG HLRAIPWLFA
WTQTRFVLPA WLGVGAGLEG ACEKGLTEDL KAMYKEWPFF QSTIDLIEMV LGKADIPIAK
HYDEVLVSQE RQELGRELRS ELMTAEKFVL VISGHEKLQQ NNRSLRRLIE NRLPFLNPLN
MLQVEILKRL RRDDDNRKIR DALLITINGI AAGMKNTG
//
