ID V7I5Z9_9CLOT Unreviewed; 449 AA.
AC V7I5Z9;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 27-MAR-2024, entry version 47.
DE SubName: Full=RNA methyltransferase {ECO:0000313|EMBL:ETA80726.1};
GN ORFNames=T472_0210330 {ECO:0000313|EMBL:ETA80726.1};
OS Youngiibacter fragilis 232.1.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Youngiibacter.
OX NCBI_TaxID=994573 {ECO:0000313|EMBL:ETA80726.1, ECO:0000313|Proteomes:UP000017747};
RN [1] {ECO:0000313|EMBL:ETA80726.1, ECO:0000313|Proteomes:UP000017747}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=232.1 {ECO:0000313|EMBL:ETA80726.1,
RC ECO:0000313|Proteomes:UP000017747};
RX PubMed=24459265;
RA Wawrik C.B., Callaghan A.V., Stamps B.W., Wawrik B.;
RT "Genome Sequence of Youngiibacter fragilis, the Type Strain of the Genus
RT Youngiibacter.";
RL Genome Announc. 2:e01183-13(2014).
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC ProRule:PRU01024}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ETA80726.1}.
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DR EMBL; AXUN02000175; ETA80726.1; -; Genomic_DNA.
DR RefSeq; WP_023387147.1; NZ_AXUN02000175.1.
DR AlphaFoldDB; V7I5Z9; -.
DR STRING; 994573.T472_0210330; -.
DR PATRIC; fig|994573.3.peg.1924; -.
DR eggNOG; COG2265; Bacteria.
DR OrthoDB; 9804590at2; -.
DR Proteomes; UP000017747; Unassembled WGS sequence.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR GO; GO:0009451; P:RNA modification; IEA:UniProt.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 2.40.50.1070; -; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR030390; MeTrfase_TrmA_AS.
DR InterPro; IPR030391; MeTrfase_TrmA_CS.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR002792; TRAM_dom.
DR InterPro; IPR010280; U5_MeTrfase_fam.
DR NCBIfam; TIGR00479; rumA; 1.
DR PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1.
DR PANTHER; PTHR11061:SF30; TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE; 1.
DR Pfam; PF01938; TRAM; 1.
DR Pfam; PF05958; tRNA_U5-meth_tr; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR PROSITE; PS50926; TRAM; 1.
DR PROSITE; PS01230; TRMA_1; 1.
DR PROSITE; PS01231; TRMA_2; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01024}; Reference proteome {ECO:0000313|Proteomes:UP000017747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01024};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01024}.
FT DOMAIN 1..58
FT /note="TRAM"
FT /evidence="ECO:0000259|PROSITE:PS50926"
FT ACT_SITE 405
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10015"
FT ACT_SITE 405
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 280
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 309
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 330
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 378
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ SEQUENCE 449 AA; 49852 MW; 1D3922334B91830F CRC64;
MKKNDIIKAD ITGQGYNGEG IARIEGMPVF IENALPGEKA SIHILKVNKN LAFGKIASID
VPSKLRVEPP CPYYWKCGGC DLQHQTYESQ LEFKKKRVRD AIERIGGVKD PVVNDTLGME
NPFRYRNKVQ LPVGLENGKI VSGFYAKRSH RIIDMESCII QHEDGDKVLK IVREWMEDKG
ILPYQADGTG LIRHIMVRKG FTTGDLMVVI VATKAELPNN EDLTRRLSSI DGFKSLILNI
NDRDTNVILG ESNIVLYGTD HIEDYISEFK FRISPHSFFQ VNPAQTQVMY SKALEYAGLT
GNEIVFDAYC GAGTISMFLS KKARKVYGVE IVREAVADAW LNAEMNGVTN IEFIEGKSED
VISDLIGQGI HADVVVVDPP RKGCERSLLE SIAAMGPKRL VYVSCDAATL GRDIGILAEL
GYELVEATPV DNFPQTAHVE TVALITRNI
//
