ID VAC8_PICPA Reviewed; 556 AA.
AC Q5EFZ4;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 22-FEB-2023, entry version 59.
DE RecName: Full=Vacuolar protein 8;
GN Name=VAC8;
OS Komagataella pastoris (Yeast) (Pichia pastoris).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Phaffomycetaceae; Komagataella.
OX NCBI_TaxID=4922;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Thomson M., Dunn W.A. Jr.;
RL Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Functions in both vacuole inheritance and protein targeting
CC from the cytoplasm to vacuole. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250}; Lipid-anchor
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the beta-catenin family. {ECO:0000305}.
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DR EMBL; AY886543; AAW78365.1; -; Genomic_DNA.
DR AlphaFoldDB; Q5EFZ4; -.
DR SMR; Q5EFZ4; -.
DR OrthoDB; 2721872at2759; -.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043495; F:protein-membrane adaptor activity; IEA:InterPro.
DR GO; GO:0071562; P:nucleus-vacuole junction assembly; IEA:InterPro.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 2.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR045156; Vac8.
DR PANTHER; PTHR47249; VACUOLAR PROTEIN 8; 1.
DR PANTHER; PTHR47249:SF1; VACUOLAR PROTEIN 8; 1.
DR Pfam; PF00514; Arm; 7.
DR Pfam; PF13513; HEAT_EZ; 1.
DR SMART; SM00185; ARM; 9.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR PROSITE; PS50176; ARM_REPEAT; 7.
PE 3: Inferred from homology;
KW Lipoprotein; Membrane; Myristate; Palmitate; Repeat; Vacuole.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..556
FT /note="Vacuolar protein 8"
FT /id="PRO_0000256216"
FT REPEAT 38..74
FT /note="ARM 1"
FT REPEAT 75..115
FT /note="ARM 2"
FT REPEAT 117..156
FT /note="ARM 3"
FT REPEAT 158..197
FT /note="ARM 4"
FT REPEAT 199..238
FT /note="ARM 5"
FT REPEAT 242..281
FT /note="ARM 6"
FT REPEAT 283..322
FT /note="ARM 7"
FT REPEAT 324..364
FT /note="ARM 8"
FT REPEAT 408..447
FT /note="ARM 9"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000250"
FT LIPID 4
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT LIPID 5
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
FT LIPID 7
FT /note="S-palmitoyl cysteine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 556 AA; 60725 MW; 6F51B8FD5390BA58 CRC64;
MGVCCSCFKR YEHDQSYPPL LAENEREAIS SLLQYLENRS EVDFFTDGPL RALSTLVYSE
NIDLQRSAAL AFAEVTEKDV RPVTRDVLEP ILILLQSSDA EVQRAACAAL GNLAVNDSNK
VLIVNMGGLE PLIRQMMSPN IEVQCNAVGC ITNLATQDQN KSKIATSGAL IPLTKLAKSK
DLRVQRNATG ALLNMTHSLE NRQELVNAGS VPILVQLLSS TDPDVQYYCT TALSNIAVDE
GNRKKLASTE PKLISQLVQL MDSTSPRVQC QATLALRNLA SDANYQLEIV RAGGLPNLVT
LLNSTHQPLV LAAVACIRNI SIHPLNEALI IDAGFLKPLV SLLDYNDNVE IQCHAVSTLR
NLAASSERNR LALLESGAVE KCEKLVLNSP ISVQSEISAC FAILALADDL KMKLLDSNII
EVLLPLTSSE NGEVCGNAAA ALANLCSRIP DYTIILKNYE QISKFIAKFL NSQNPTFEHI
ALWTTLQLLE SEDETIKAKL KKQINSGEIS LNNLTTSTLN NHSDTSNIIN NDGEEGEFND
GDDIINLTQQ ILEMIK
//
