ID   W0RF50_9BACT            Unreviewed;       524 AA.
AC   W0RF50;
DT   19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT   19-MAR-2014, sequence version 1.
DT   27-MAR-2024, entry version 30.
DE   SubName: Full=Type I phosphodiesterase/nucleotide pyrophosphatase {ECO:0000313|EMBL:AHG88965.1};
GN   ORFNames=J421_1428 {ECO:0000313|EMBL:AHG88965.1};
OS   Gemmatirosa kalamazoonensis.
OC   Bacteria; Gemmatimonadota; Gemmatimonadetes; Gemmatimonadales;
OC   Gemmatimonadaceae; Gemmatirosa.
OX   NCBI_TaxID=861299 {ECO:0000313|EMBL:AHG88965.1, ECO:0000313|Proteomes:UP000019151};
RN   [1] {ECO:0000313|EMBL:AHG88965.1, ECO:0000313|Proteomes:UP000019151}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KBS708 {ECO:0000313|EMBL:AHG88965.1,
RC   ECO:0000313|Proteomes:UP000019151};
RX   PubMed=24699952;
RA   Debruyn J.M., Radosevich M., Wommack K.E., Polson S.W., Hauser L.J.,
RA   Fawaz M.N., Korlach J., Tsai Y.C.;
RT   "Genome Sequence and Methylome of Soil Bacterium Gemmatirosa
RT   kalamazoonensis KBS708T, a Member of the Rarely Cultivated Gemmatimonadetes
RT   Phylum.";
RL   Genome Announc. 2:e00226-14(2014).
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DR   EMBL; CP007128; AHG88965.1; -; Genomic_DNA.
DR   AlphaFoldDB; W0RF50; -.
DR   STRING; 861299.J421_1428; -.
DR   KEGG; gba:J421_1428; -.
DR   eggNOG; COG1524; Bacteria.
DR   HOGENOM; CLU_034095_0_0_0; -.
DR   InParanoid; W0RF50; -.
DR   OrthoDB; 9766127at2; -.
DR   Proteomes; UP000019151; Chromosome.
DR   GO; GO:0004035; F:alkaline phosphatase activity; IEA:InterPro.
DR   Gene3D; 3.30.1360.150; -; 1.
DR   Gene3D; 3.40.720.10; Alkaline Phosphatase, subunit A; 1.
DR   InterPro; IPR017850; Alkaline_phosphatase_core_sf.
DR   InterPro; IPR026263; Alkaline_phosphatase_prok.
DR   InterPro; IPR002591; Phosphodiest/P_Trfase.
DR   PANTHER; PTHR10151:SF120; BIS(5'-ADENOSYL)-TRIPHOSPHATASE; 1.
DR   PANTHER; PTHR10151; ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE; 1.
DR   Pfam; PF01663; Phosphodiest; 1.
DR   PIRSF; PIRSF031924; Pi-irrepressible_AP; 1.
DR   SUPFAM; SSF53649; Alkaline phosphatase-like; 1.
PE   4: Predicted;
KW   Phosphoprotein {ECO:0000256|PIRSR:PIRSR031924-50};
KW   Reference proteome {ECO:0000313|Proteomes:UP000019151};
KW   Signal {ECO:0000256|SAM:SignalP}.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT   CHAIN           22..524
FT                   /evidence="ECO:0000256|SAM:SignalP"
FT                   /id="PRO_5004794030"
FT   ACT_SITE        77
FT                   /note="Phosphothreonine intermediate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR031924-50"
FT   BINDING         98
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR031924-51"
FT   BINDING         147..149
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR031924-51"
SQ   SEQUENCE   524 AA;  57059 MW;  8FBA27D1C49C2AAE CRC64;
     MRHRLLAAAL GVALGAAPLG AQRPTQRPTL VVFFTIDQGT PVYFTRFGSQ LTGGLKRLWD
     GGAVFTDANQ DHGTTETAPG HASVMSGRFP SHTGIVRNNA GVQDPQAPVI GAPKADPVSP
     FRFRGTVLTD WMRTADPRTR ALSVSRKDRG AILPMGRAHQ TVLWYGGNGT FTTSTYYADT
     LPTWVRQFNA RGGGPLKRFA GQTWDLLLPA SQYGEPDSVA LENGGKAYTF PHVFPTDSAA
     EAGELTNYPW MDQLTLDLAL EGLQQLKLGT GPAPDVLAVS LSTTDAVGHR YGMDSREMHD
     HILRLDRALG AFLDSLYKLR DSSRVIIALT ADHGMAPYPE LHFAANPSQG RADVAPVLQR
     VKQALDDRHV PRDAFDFESG VVLLDKPAVT RAGVNADSVV RALVADLSKV KGVMRVYRRE
     ELASLASRDK YARRWLHMTP DDLDAVAYVT LEPYVYYAQT QYATHGSPHD YDTRVSLIFY
     GPPFKPGKYG AFVRTVDIAP TLAAVLGVPP TEPIDGVVLR AALK
//