ID W1NV36_AMBTC Unreviewed; 461 AA.
AC W1NV36;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 24-JAN-2024, entry version 41.
DE RecName: Full=digalactosyldiacylglycerol synthase {ECO:0000256|ARBA:ARBA00024055};
DE EC=2.4.1.241 {ECO:0000256|ARBA:ARBA00024055};
GN ORFNames=AMTR_s00101p00147880 {ECO:0000313|EMBL:ERM99120.1};
OS Amborella trichopoda.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Amborellales; Amborellaceae; Amborella.
OX NCBI_TaxID=13333 {ECO:0000313|EMBL:ERM99120.1, ECO:0000313|Proteomes:UP000017836};
RN [1] {ECO:0000313|Proteomes:UP000017836}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=24357323;
RG Amborella Genome Project;
RT "The Amborella genome and the evolution of flowering plants.";
RL Science 342:1241089-1241089(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol + UDP-alpha-
CC D-galactose = 1,2-diacyl-3-O-[alpha-D-galactosyl-(1->6)-beta-D-
CC galactosyl]-sn-glycerol + H(+) + UDP; Xref=Rhea:RHEA:10520,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17615, ChEBI:CHEBI:28396,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:66914; EC=2.4.1.241;
CC Evidence={ECO:0000256|ARBA:ARBA00023914};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004370}.
CC Plastid, chloroplast outer membrane {ECO:0000256|ARBA:ARBA00024013}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC Glycosyltransferase 4 subfamily. {ECO:0000256|ARBA:ARBA00009481}.
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DR EMBL; KI395058; ERM99120.1; -; Genomic_DNA.
DR AlphaFoldDB; W1NV36; -.
DR STRING; 13333.W1NV36; -.
DR EnsemblPlants; ERM99120; ERM99120; AMTR_s00101p00147880.
DR Gramene; ERM99120; ERM99120; AMTR_s00101p00147880.
DR eggNOG; ENOG502QQEJ; Eukaryota.
DR HOGENOM; CLU_011647_1_0_1; -.
DR OMA; APLTDMQ; -.
DR Proteomes; UP000017836; Unassembled WGS sequence.
DR GO; GO:0009707; C:chloroplast outer membrane; IBA:GO_Central.
DR GO; GO:0046481; F:digalactosyldiacylglycerol synthase activity; IEA:InterPro.
DR GO; GO:0035250; F:UDP-galactosyltransferase activity; IBA:GO_Central.
DR GO; GO:0016036; P:cellular response to phosphate starvation; IEA:EnsemblPlants.
DR GO; GO:0019375; P:galactolipid biosynthetic process; IBA:GO_Central.
DR CDD; cd01635; Glycosyltransferase_GTB-type; 1.
DR Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 1.
DR InterPro; IPR044525; DGDG1/2.
DR InterPro; IPR001296; Glyco_trans_1.
DR PANTHER; PTHR46132; DIGALACTOSYLDIACYLGLYCEROL SYNTHASE 2, CHLOROPLASTIC; 1.
DR PANTHER; PTHR46132:SF1; DIGALACTOSYLDIACYLGLYCEROL SYNTHASE 2, CHLOROPLASTIC; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Membrane {ECO:0000256|ARBA:ARBA00022805};
KW Plastid {ECO:0000256|ARBA:ARBA00022805};
KW Plastid outer membrane {ECO:0000256|ARBA:ARBA00022805};
KW Reference proteome {ECO:0000313|Proteomes:UP000017836};
KW Transferase {ECO:0000256|ARBA:ARBA00022676}.
FT DOMAIN 208..315
FT /note="Glycosyl transferase family 1"
FT /evidence="ECO:0000259|Pfam:PF00534"
SQ SEQUENCE 461 AA; 52345 MW; 712FD4AF18C84B8E CRC64;
MTGTAVNPLF RAAYLAKAGH GRVTLFIPWL SLKEQELVYP SGITFSTPLE QEAYIRQWLE
EKTGFSSDFN IRFYPGRFST DKRSILAVGD NTELVPDEEA DIAVLEEPEH LTWYHHGRSW
KTKFRRVIGV VHTNYIEYVK REKNGRVQAF FLNYVNNWVT QIYCHKVIRL SAATQDLPRS
VICNVHGVGP KFLEVGLKKQ EQQKLGGRTF SKGAYYIGKM VWSKGYKELL QLLSEHQSKL
ADFEIDLYGN GEDSVQIREA ARKLSLTINV YPGRDHADPL FHEYKVFINP STTDVVCTTT
AEALAMGKIV ICADHVSNDF FREFPNCRTY KGSNGFPPIV RAAMESEPLP LSDELRHRLS
WEAATERFVK AAELNEQSLG GKVVSSYPSK GFMSISFEMS DIRRFVDDAS AFMHNAASGI
EPARRAFGAI PKTLYPDEQQ CRELGLKPNG TNKSGKQWFK R
//
