ID W1P2L4_AMBTC Unreviewed; 336 AA.
AC W1P2L4;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE RecName: Full=tRNA pseudouridine synthase {ECO:0000256|RuleBase:RU003792};
DE EC=5.4.99.12 {ECO:0000256|RuleBase:RU003792};
GN ORFNames=AMTR_s00077p00023510 {ECO:0000313|EMBL:ERN04102.1};
OS Amborella trichopoda.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Amborellales; Amborellaceae; Amborella.
OX NCBI_TaxID=13333 {ECO:0000313|EMBL:ERN04102.1, ECO:0000313|Proteomes:UP000017836};
RN [1] {ECO:0000313|Proteomes:UP000017836}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=24357323;
RG Amborella Genome Project;
RT "The Amborella genome and the evolution of flowering plants.";
RL Science 342:1241089-1241089(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA;
CC Xref=Rhea:RHEA:22376, Rhea:RHEA-COMP:10085, Rhea:RHEA-COMP:10087,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.12;
CC Evidence={ECO:0000256|RuleBase:RU003792};
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC {ECO:0000256|ARBA:ARBA00009375, ECO:0000256|RuleBase:RU003792}.
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DR EMBL; KI394293; ERN04102.1; -; Genomic_DNA.
DR AlphaFoldDB; W1P2L4; -.
DR STRING; 13333.W1P2L4; -.
DR EnsemblPlants; ERN04102; ERN04102; AMTR_s00077p00023510.
DR Gramene; ERN04102; ERN04102; AMTR_s00077p00023510.
DR eggNOG; KOG2553; Eukaryota.
DR HOGENOM; CLU_023331_0_0_1; -.
DR OMA; YVGTDFR; -.
DR Proteomes; UP000017836; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.660; Pseudouridine synthase I, catalytic domain, C-terminal subdomain; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR PANTHER; PTHR11142; PSEUDOURIDYLATE SYNTHASE; 1.
DR PANTHER; PTHR11142:SF9; TRNA PSEUDOURIDYLATE SYNTHASE; 1.
DR Pfam; PF01416; PseudoU_synth_1; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 2.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|RuleBase:RU003792};
KW Reference proteome {ECO:0000313|Proteomes:UP000017836};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694,
KW ECO:0000256|RuleBase:RU003792}.
FT DOMAIN 193..272
FT /note="Pseudouridine synthase I TruA alpha/beta"
FT /evidence="ECO:0000259|Pfam:PF01416"
FT REGION 111..160
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 143..159
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 336 AA; 38216 MW; 5E7C31B9F8D478E5 CRC64;
MISLKMEIPE NAWNRDPNGI NMANTINAHL PNNMKVFGVL PSQRSFDARR ECIARMYSYL
LPAEIIGITN QCGPGEIERH LLEFNNILKT FEGDHPFHNY TARSKYRRRH CGGSNFSKGT
ISSKEKSDAE DEQSDEEEES GRATTDNEQS DDKGLDYSEM PDNNFELSND VAAPRVRAKW
VHEPDEKDLI SGSHFRKIFS FTCGKLETSN EMPFLEICVC GESFMLHQIR KMVAAAIAVK
RALLPHDVID LSLTKFSRMV LPIAPSEVLI LKGNKYSLRN LPCGSKPEVF RLIESKEINE
AIENFYSSVL LPQVSSAFLD PSRPPWMDWV CKYTRC
//
