ID W1WUT1_9ZZZZ Unreviewed; 391 AA.
AC W1WUT1;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 25.
DE RecName: Full=diglucosyl diacylglycerol synthase (1,6-linking) {ECO:0000256|ARBA:ARBA00012817};
DE EC=2.4.1.315 {ECO:0000256|ARBA:ARBA00012817};
DE AltName: Full=Diglucosyl diacylglycerol synthase (1,6-linking) {ECO:0000256|ARBA:ARBA00030341};
GN ORFNames=Q604_UNBC18433G0013 {ECO:0000313|EMBL:ETJ20204.1};
OS human gut metagenome.
OC unclassified sequences; metagenomes; organismal metagenomes.
OX NCBI_TaxID=408170 {ECO:0000313|EMBL:ETJ20204.1};
RN [1] {ECO:0000313|EMBL:ETJ20204.1}
RP NUCLEOTIDE SEQUENCE.
RA Brown C.T., Sharon I., Thomas B.C., Castelle C.J., Morowitz M.J.,
RA Banfield J.F.;
RT "A Varibaculum cambriense genome reconstructed from a premature infant gut
RT community with otherwise low bacterial novelty that shifts toward anaerobic
RT metabolism during the third week of life.";
RL Submitted (DEC-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol + UDP-
CC alpha-D-glucose = a 1,2-diacyl-3-O-(beta-D-Glc-(1->6)-beta-D-Glc)-sn-
CC glycerol + H(+) + UDP; Xref=Rhea:RHEA:39031, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:75799,
CC ChEBI:CHEBI:76264; EC=2.4.1.315;
CC Evidence={ECO:0000256|ARBA:ARBA00000421};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004370}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 28 family.
CC {ECO:0000256|ARBA:ARBA00006962}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ETJ20204.1}.
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DR EMBL; AZMM01018433; ETJ20204.1; -; Genomic_DNA.
DR AlphaFoldDB; W1WUT1; -.
DR SMR; W1WUT1; -.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-KW.
DR GO; GO:0047228; F:1,2-diacylglycerol 3-glucosyltransferase activity; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0009247; P:glycolipid biosynthetic process; IEA:InterPro.
DR CDD; cd17507; GT28_Beta-DGS-like; 1.
DR Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 1.
DR HAMAP; MF_01280; Diacylglyc_glucosyltr; 1.
DR InterPro; IPR009695; Diacylglyc_glucosyltr_N.
DR InterPro; IPR007235; Glyco_trans_28_C.
DR InterPro; IPR023589; Pro_diacylglycrl_glcsylTrfase.
DR PANTHER; PTHR43025; MONOGALACTOSYLDIACYLGLYCEROL SYNTHASE; 1.
DR PANTHER; PTHR43025:SF3; MONOGALACTOSYLDIACYLGLYCEROL SYNTHASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF04101; Glyco_tran_28_C; 1.
DR Pfam; PF06925; MGDG_synth; 1.
DR SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Membrane {ECO:0000256|ARBA:ARBA00022475};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:ETJ20204.1}.
FT DOMAIN 19..179
FT /note="Diacylglycerol glucosyltransferase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF06925"
FT DOMAIN 227..349
FT /note="Glycosyl transferase family 28 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF04101"
SQ SEQUENCE 391 AA; 44762 MW; D93A7999118DFA60 CRC64;
MVTQNKKILI ITGSFGNGHM QVTQSIVNQL NEMNLNHLSV IQHDLFMEAH PIMTSICKKW
YINSFKYFRN TYKRFYYSRP NELDKCFYKY YGLNKLINLL IKEKPDLILL TFPTPVMSVL
TEQFNINIPI ATVMTDYRMH KNWITPYSQR YYVATKDTKD DFIEAGVPAS YIKVTGIPIA
DKFEESIDKE EWLSQQHLDP SKPTILMSAG AFGVSKGFDY MINNILEKSP NSQVVMICGR
SKELKRSLKA KFKDNPSVII LGYTNHMNEW MASSQLMITK PGGITISEGL SRCIPMIFLN
PAPGQELENA YYFESKGFGK IADTPNEAID IVSDLTNNEE TLKVMSSKML ESKVGYSTRK
ICKDLLDLIG HSSQPDEIYG KVPLYARFFV K
//
