ID W2GX33_PHYPR Unreviewed; 642 AA.
AC W2GX33;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=L915_08569 {ECO:0000313|EMBL:ETK86880.1};
OS Phytophthora parasitica (Potato buckeye rot agent).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=4792 {ECO:0000313|EMBL:ETK86880.1};
RN [1] {ECO:0000313|EMBL:ETK86880.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CJ02B3 {ECO:0000313|EMBL:ETK86880.1};
RG The Broad Institute Genomics Platform;
RA Russ C., Tyler B., Panabieres F., Shan W., Tripathy S., Grunwald N.,
RA Machado M., Johnson C.S., Arredondo F., Hong C., Coffey M., Young S.K.,
RA Zeng Q., Gargeya S., Fitzgerald M., Abouelleil A., Alvarado L.,
RA Chapman S.B., Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M.,
RA Howarth C., Imamovic A., Ireland A., Larimer J., McCowan C., Murphy C.,
RA Pearson M., Poon T.W., Priest M., Roberts A., Saif S., Shea T., Sykes S.,
RA Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Phytophthora parasitica CJ02B3.";
RL Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
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DR EMBL; KI686237; ETK86880.1; -; Genomic_DNA.
DR AlphaFoldDB; W2GX33; -.
DR EnsemblProtists; ETK86880; ETK86880; L915_08569.
DR VEuPathDB; FungiDB:PPTG_11086; -.
DR Proteomes; UP000053236; Unassembled WGS sequence.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR CDD; cd00078; HECTc; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR PANTHER; PTHR11254:SF446; HECT-TYPE E3 UBIQUITIN TRANSFERASE; 1.
DR Pfam; PF00632; HECT; 1.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR PROSITE; PS50237; HECT; 1.
PE 4: Predicted;
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 291..642
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT ACT_SITE 609
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 642 AA; 72569 MW; 37AE5ABFB9B3ED83 CRC64;
MLPPPIGKQP RKSFQLLTSE STESQAELAA RNRSFHVRRL NEMNLNQRQR GARRRHLWQR
ERTSDGQFRW VRVGHCQPSR ISQLASNIRA SLPTSLLPAA GTENERVSAP AAYTFDEESH
SPTSPISLTR TSQGLNREDL GVETELTMVE STHADGAYSP AIRKNVRGCR SPVNCDDLTA
SAVSTDDGLI AQPSVGYVRH VNEHGHAEWV AADSLVHERA AMTAIDIEEF PRATSYIDFE
SVAALPFKAK VRWFLKELGK VAVPWDEGHL LLKIRREAVL SESMHLLMLV PAADLRQRLR
IEFIDEPGLD AGGLLREWVL LLCEQLFDES YGLFQRTHAD QSGYWINPNS RDIHRDHLHC
FQFVGRLLAK CLLEGQLLTV HLALPLLKHL LGVPISFSDL EFLDAELHRH ALWLRENEGV
EALALDFTAQ RQGTDGAIIT EELKPGGKDI PVTDANKEEY LTLLLKHKMF GAVREQLEAM
LQGLYDVLPR TLLAVFDYQE LELLVCGVPS IDVADWESHT DVRYMRAEQG LNRPAASERQ
VVKWFWEAVR AFSQEERARL LQFVTGTSRV PAEGFRALLS HDGRIRRFGL QMVAIGAPPA
GLYPKAHTCF NRLDLPVYRS YEELVTYLTL VINMEITGFT MQ
//
