ID W2P866_PHYPR Unreviewed; 686 AA.
AC W2P866;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=L914_00798 {ECO:0000313|EMBL:ETM56159.1};
OS Phytophthora parasitica (Potato buckeye rot agent).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=4792 {ECO:0000313|EMBL:ETM56159.1};
RN [1] {ECO:0000313|EMBL:ETM56159.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IAC_01/95 {ECO:0000313|EMBL:ETM56159.1};
RG The Broad Institute Genomics Platform;
RA Russ C., Tyler B., Panabieres F., Shan W., Tripathy S., Grunwald N.,
RA Machado M., Johnson C.S., Arredondo F., Hong C., Coffey M., Young S.K.,
RA Zeng Q., Gargeya S., Fitzgerald M., Abouelleil A., Alvarado L.,
RA Chapman S.B., Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M.,
RA Howarth C., Imamovic A., Ireland A., Larimer J., McCowan C., Murphy C.,
RA Pearson M., Poon T.W., Priest M., Roberts A., Saif S., Shea T., Sykes S.,
RA Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Phytophthora parasitica IAC_01/95.";
RL Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
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DR EMBL; KI690562; ETM56159.1; -; Genomic_DNA.
DR AlphaFoldDB; W2P866; -.
DR EnsemblProtists; ETM56159; ETM56159; L914_00798.
DR VEuPathDB; FungiDB:PPTG_00734; -.
DR Proteomes; UP000054532; Unassembled WGS sequence.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR CDD; cd00078; HECTc; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR PANTHER; PTHR11254:SF446; HECT-TYPE E3 UBIQUITIN TRANSFERASE; 1.
DR Pfam; PF00632; HECT; 1.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR PROSITE; PS50237; HECT; 1.
PE 4: Predicted;
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 349..686
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT ACT_SITE 653
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 686 AA; 77447 MW; 16FF3A5EDC12571E CRC64;
MEAVLGFLVV LAIIALLFFF LSMLARRAYI RRQRSLELHA PLLNSDDRRL DAALRDAEQG
FNECTVCSFE NFKRFKFCTV CGEAIVSDEQ DGDRDMNISK KRSTRLSFLP LLKRETKVEL
VTVTPPKQTT RRRLRAQRRK EWTRKIDVAG CAFWFRDGRW GDTQFTGFVV KFQSTPAMAG
NGMEKLLPNA VGEGESSLPP SECGTDVVTV ASSVKMDTPD PNPVSAEVNT ESVEQDSTQQ
PLVGSIDGCI ATLIRGTQEQ HVVLTPSTDT TVDASKLPLG NSTSTEDNAS TPLTEIMALA
AKDFPSKYAH FVTATSALLI PAEKEHLRFN VERANVFEDS MESLAIIPLQ NVHSVLRINF
LDESGVDAGG LHREWFVLLN EMLVDPARGL FVCTNENEQT YFLNPCFTAN SLPDQLLHLY
ATGRLLGRAL LEGSMLCFHL APPLLKLMLG YPLSFADVED LDPEVYTNLR WLLDNENVEL
IGLYFTVTVK EEEKYRDVEL VPGGSSIAVT DSNKREYVER KWQYLLVESV APQLQVFLCG
LYEIVPRELL LLFDPEEFDF LLCGSDEIDV DDWERNTKYS EELHHHRSLI WFWELVREMP
NEYRRRLLQF STGSSRVPLG GFSALMSYDG RLCPFTLKAV SLVGDGFIHS HACFNRLDLP
LHVVRDELKA VLYATLETEV YGFTTD
//
