ID W2QN94_PHYPN Unreviewed; 1001 AA.
AC W2QN94;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=subtilisin {ECO:0000256|ARBA:ARBA00023619};
DE EC=3.4.21.62 {ECO:0000256|ARBA:ARBA00023619};
GN ORFNames=PPTG_07472 {ECO:0000313|EMBL:ETN14421.1};
OS Phytophthora parasitica (strain INRA-310).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=761204 {ECO:0000313|EMBL:ETN14421.1, ECO:0000313|Proteomes:UP000018817};
RN [1] {ECO:0000313|Proteomes:UP000018817}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=INRA-310 {ECO:0000313|Proteomes:UP000018817};
RG The Broad Institute Genome Sequencing Platform;
RA Russ C., Tyler B., Panabieres F., Shan W., Tripathy S., Grunwald N.,
RA Machado M., Young S.K., Zeng Q., Gargeya S., Fitzgerald M., Haas B.,
RA Abouelleil A., Alvarado L., Arachchi H.M., Berlin A., Chapman S.B.,
RA Gearin G., Goldberg J., Griggs A., Gujja S., Hansen M., Heiman D.,
RA Howarth C., Larimer J., Lui A., MacDonald P.J.P., McCowen C.,
RA Montmayeur A., Murphy C., Neiman D., Pearson M., Priest M., Roberts A.,
RA Saif S., Shea T., Sisk P., Stolte C., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RL Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ETN14421.1, ECO:0000313|Proteomes:UP000018817}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=INRA-310 {ECO:0000313|EMBL:ETN14421.1,
RC ECO:0000313|Proteomes:UP000018817};
RG The Broad Institute Genomics Platform;
RA Russ C., Tyler B., Panabieres F., Shan W., Tripathy S., Grunwald N.,
RA Machado M., Johnson C.S., Arredondo F., Hong C., Coffey M., Young S.K.,
RA Zeng Q., Gargeya S., Fitzgerald M., Abouelleil A., Alvarado L.,
RA Chapman S.B., Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M.,
RA Howarth C., Imamovic A., Ireland A., Larimer J., McCowan C., Murphy C.,
RA Pearson M., Poon T.W., Priest M., Roberts A., Saif S., Shea T., Sykes S.,
RA Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Phytophthora parasitica INRA-310.";
RL Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of proteins with broad specificity for peptide
CC bonds, and a preference for a large uncharged residue in P1.
CC Hydrolyzes peptide amides.; EC=3.4.21.62;
CC Evidence={ECO:0000256|ARBA:ARBA00023529};
CC -!- SIMILARITY: Belongs to the peptidase S8 family.
CC {ECO:0000256|ARBA:ARBA00011073, ECO:0000256|PROSITE-ProRule:PRU01240}.
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DR EMBL; KI669572; ETN14421.1; -; Genomic_DNA.
DR RefSeq; XP_008899999.1; XM_008901751.1.
DR AlphaFoldDB; W2QN94; -.
DR STRING; 761204.W2QN94; -.
DR EnsemblProtists; ETN14421; ETN14421; PPTG_07472.
DR GeneID; 20177342; -.
DR VEuPathDB; FungiDB:PPTG_07472; -.
DR OMA; ICRAMPC; -.
DR OrthoDB; 2441948at2759; -.
DR Proteomes; UP000018817; Unassembled WGS sequence.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008240; F:tripeptidyl-peptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd04857; Peptidases_S8_Tripeptidyl_Aminopeptidase_II; 1.
DR Gene3D; 1.25.40.710; -; 1.
DR Gene3D; 2.60.40.3170; -; 1.
DR Gene3D; 3.40.50.200; Peptidase S8/S53 domain; 2.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR022398; Peptidase_S8_His-AS.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR034051; TPP_II_domain.
DR InterPro; IPR046939; TPPII_C_sf.
DR InterPro; IPR048384; TPPII_GBD.
DR InterPro; IPR048383; TPPII_Ig-like-1.
DR InterPro; IPR046940; TPPII_Ig-like_sf.
DR PANTHER; PTHR43806; PEPTIDASE S8; 1.
DR PANTHER; PTHR43806:SF14; TRIPEPTIDYL-PEPTIDASE 2; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR Pfam; PF21316; TPPII_GBD; 1.
DR Pfam; PF21223; TPPII_Ig-like-1; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52743; Subtilisin-like; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00137; SUBTILASE_HIS; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU01240};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PROSITE-
KW ProRule:PRU01240}; Reference proteome {ECO:0000313|Proteomes:UP000018817};
KW Serine protease {ECO:0000256|ARBA:ARBA00022825, ECO:0000256|PROSITE-
KW ProRule:PRU01240}.
FT DOMAIN 29..497
FT /note="Peptidase S8/S53"
FT /evidence="ECO:0000259|Pfam:PF00082"
FT DOMAIN 523..640
FT /note="Tripeptidyl-peptidase II first Ig-like"
FT /evidence="ECO:0000259|Pfam:PF21223"
FT DOMAIN 655..751
FT /note="Tripeptidyl-peptidase II galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21316"
FT ACT_SITE 38
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01240"
FT ACT_SITE 260
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01240"
FT ACT_SITE 446
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01240"
SQ SEQUENCE 1001 AA; 110580 MW; FFAADC6B17E849B0 CRC64;
MKSFPTASLL PKEETLADRF LQQYPDYDGR NAVVAIFDTG VDPGAIGLQT TPDGRPKIID
VVDATGAGDV DTSTVIEAKD GQLTLANDRV LTLNPDWKPS QDGKYHVGTV AGYHLFPGPL
VTRLKTERKE KFDIEQRAAV NAVQEQLAKW SKENSATTND TAKLREKKDL QTRLKQLEEL
AKSYEDPGPI YDAVVFYDGT CWRAALDTKE TGDFTGIPVL TNFKDERQYA TFSGESQLNY
VLNIYDEGNT LSVVNDVGAH GTHVAGIVAA YHPDQSECNG VAPGAQIVAV KIGDGRLGSM
ETSSALSRAI LAVMDANVDV VNMSYGEYAS QHNYGRIVEL SNELVDEHNV TFVVSAGNNG
PALGTVGAPG GTTSSMLAVG AYVSPKMMEG EYIMRDSALS GIAYTWSSRG PTFDGDLGVN
ICAPGAAIAP VPNWTLNKKQ LMNGTSMSSP NCAGNIALLI SAMKAQGVEY TPYSIRRALE
NTAVKVPNVE VYAQGKGLIQ VLPAFKYLAG SNSFDGGRKF PLHYEIKTSS GDGKARGIFL
RDSVDFAHDS TEVNVTVTPK FHKKAVQDDK VHFEQHVRLV PSARWIDVGR NLALMHGGRA
FKVLVKTKHL TAGEHYGEIV AYDTKNEARG ALFTIPVTVI KPEDAKSVVT YQTKFQPGDI
SRRFFTPAQD STWADIIFSR ANENDREVES NASGKLYMFD ALQFQPFVRQ SSSSFHKAFF
LKPGEEVAFS MDLLGGLTTE FCLGQFWSAL GDSIVQIEIR FHGVKPDQEK IVPKDERDEE
EIANEAVRDL LLERVTKLVG KSTFLPAWQR LVDQFPNYLP AMQAKLHHFD FEANRSTQLA
AVIEAADAVL ALIKQDELAL FFGTRSVPGD QPATEKKLRK EKEKAILVDA LARKARALGD
SSQWDDFLLA YADLQKWEDV EAATYLHVSL LHDRHFDAFG LALQRLRKVQ DMEQADKTKI
ISDEKLAAEI NSTLDALQWQ HWTKYETQWE RRRAPTSYRI F
//
