ID   W4VMV4_9BACI            Unreviewed;       293 AA.
AC   W4VMV4;
DT   19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT   19-MAR-2014, sequence version 1.
DT   27-MAR-2024, entry version 17.
DE   SubName: Full=Beta-xylosidase {ECO:0000313|EMBL:GAE94456.1};
GN   ORFNames=JCM21714_3613 {ECO:0000313|EMBL:GAE94456.1};
OS   Gracilibacillus boraciitolerans JCM 21714.
OC   Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Gracilibacillus.
OX   NCBI_TaxID=1298598 {ECO:0000313|EMBL:GAE94456.1, ECO:0000313|Proteomes:UP000019102};
RN   [1] {ECO:0000313|EMBL:GAE94456.1, ECO:0000313|Proteomes:UP000019102}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JCM 21714 {ECO:0000313|EMBL:GAE94456.1,
RC   ECO:0000313|Proteomes:UP000019102};
RA   Ahmed I., Oshima K., Suda W., Kitamura K., Iida T., Ohmori Y., Fujiwara T.,
RA   Hattori M., Ohkuma M.;
RT   "Draft Genome Sequence of the Boron-Tolerant and Moderately Halotolerant
RT   Bacterium Gracilibacillus boraciitolerans JCM 21714T.";
RL   Genome Announc. 2:e00097-14(2014).
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC       {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:GAE94456.1}.
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DR   EMBL; BAVS01000024; GAE94456.1; -; Genomic_DNA.
DR   AlphaFoldDB; W4VMV4; -.
DR   STRING; 1298598.JCM21714_3613; -.
DR   eggNOG; COG3507; Bacteria.
DR   Proteomes; UP000019102; Unassembled WGS sequence.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   CDD; cd09001; GH43_FsAxh1-like; 1.
DR   InterPro; IPR006710; Glyco_hydro_43.
DR   InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR   PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR   PANTHER; PTHR42812:SF13; HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G00930)-RELATED; 1.
DR   Pfam; PF04616; Glyco_hydro_43; 1.
DR   SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
PE   3: Inferred from homology;
KW   Glycosidase {ECO:0000256|RuleBase:RU361187};
KW   Hydrolase {ECO:0000256|RuleBase:RU361187};
KW   Reference proteome {ECO:0000313|Proteomes:UP000019102}.
FT   ACT_SITE        17
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   ACT_SITE        181
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT   SITE            128
FT                   /note="Important for catalytic activity, responsible for
FT                   pKa modulation of the active site Glu and correct
FT                   orientation of both the proton donor and substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ   SEQUENCE   293 AA;  33963 MW;  A6BA90EE88CF573B CRC64;
     MNKITLTNPV FWSDVPDIDV IRVEDAFYMV STSMHSMPGC PIMKSFNLRD WEIVSYVYDT
     LEDNKAHVLL DEKGIYGQGQ WATSLRYHNE TFYLCFSSND MKQFYVYQTI DIEKGPWTRS
     VIEGIYHDPA LLFDEDRVFV IYGCGDIRIT ELTSDLSDVK KGGIDQLLLE TPKENIGLRC
     EGCHAYKIDE VYYLLFIEWP TDGNARRRQV CYRSTELLGD YERKVVLDDD MGYHNKGIAQ
     GGAIFDTPDH KWYAMLFQDH DAVGRIPYVL PVEWKDGWPM IGIAGKAPRI HRH
//