ID W5IIB2_SCAIO Unreviewed; 778 AA.
AC W5IIB2;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE RecName: Full=starch synthase (maltosyl-transferring) {ECO:0000256|ARBA:ARBA00012603};
DE EC=2.4.99.16 {ECO:0000256|ARBA:ARBA00012603};
GN ORFNames=HMPREF9020_00239 {ECO:0000313|EMBL:EFG26614.1};
OS Scardovia inopinata F0304.
OC Bacteria; Actinomycetota; Actinomycetes; Bifidobacteriales;
OC Bifidobacteriaceae; Scardovia.
OX NCBI_TaxID=641146 {ECO:0000313|EMBL:EFG26614.1, ECO:0000313|Proteomes:UP000005777};
RN [1] {ECO:0000313|EMBL:EFG26614.1, ECO:0000313|Proteomes:UP000005777}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=F0304 {ECO:0000313|EMBL:EFG26614.1,
RC ECO:0000313|Proteomes:UP000005777};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Izard J., Baranova O.V.,
RA Blanton J.M., Tanner A.C., Dewhirst F.E., Young S.K., Zeng Q., Gargeya S.,
RA Fitzgerald M., Haas B., Abouelleil A., Alvarado L., Arachchi H.M.,
RA Berlin A., Chapman S.B., Gearin G., Goldberg J., Griggs A., Gujja S.,
RA Hansen M., Heiman D., Howarth C., Larimer J., Lui A., MacDonald P.J.,
RA McCowen C., Montmayeur A., Murphy C., Neiman D., Pearson M., Priest M.,
RA Roberts A., Saif S., Shea T., Sisk P., Stolte C., Sykes S., Wortman J.,
RA Nusbaum C., Birren B.;
RT "The Genome Sequence of Scardovia inopinata F0304.";
RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[(1->4)-alpha-D-glucosyl](n) + alpha-maltose 1-phosphate =
CC [(1->4)-alpha-D-glucosyl](n+2) + phosphate; Xref=Rhea:RHEA:42692,
CC Rhea:RHEA-COMP:9584, Rhea:RHEA-COMP:10183, ChEBI:CHEBI:15444,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:63576; EC=2.4.99.16;
CC Evidence={ECO:0000256|ARBA:ARBA00000575};
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EFG26614.1}.
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DR EMBL; ADCX01000002; EFG26614.1; -; Genomic_DNA.
DR RefSeq; WP_006292578.1; NZ_GG770225.1.
DR AlphaFoldDB; W5IIB2; -.
DR eggNOG; COG0366; Bacteria.
DR HOGENOM; CLU_015798_0_0_11; -.
DR Proteomes; UP000005777; Unassembled WGS sequence.
DR GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR Gene3D; 1.20.58.80; Phosphotransferase system, lactose/cellobiose-type IIA subunit; 1.
DR InterPro; IPR049171; GLGE_C.
DR InterPro; IPR021828; GlgE_dom_N/S.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR PANTHER; PTHR47786:SF2; AAMY DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR47786; ALPHA-1,4-GLUCAN:MALTOSE-1-PHOSPHATE MALTOSYLTRANSFERASE; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF21702; GLGE_C; 1.
DR Pfam; PF11896; GlgE_dom_N_S; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE 4: Predicted;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Reference proteome {ECO:0000313|Proteomes:UP000005777};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 310..660
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT REGION 1..89
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 29..78
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 778 AA; 86608 MW; 37DC6F158DA96431 CRC64;
MAATAQSQSE RESPAEAGVK KRTRARKAQS HTQPVSAAHT GAKKSISRAG STTESLKSSR
KNGGKSGLKS SKTGKSKKSA AASPFDRKNP QEFGRVNVIN VSPQTSNGFL PRVEIGELVD
VQANVFIEGR TRVGASAVVY TKTGALFQRV PLTCTVAGLD QWNGTVRMGK AELALPWEEG
FAAVQRRLGI WKLVVEGWQD TYATWLHDAR IKIAAHDDQE NVLDEGQEIL KRWAASKDAH
LTVAEKKTLR LAIALIDDDT MTYDDRLAAA DNSDLHALHD HKPLREGISE SNPVNFMVER
PHSSFASWYQ FFPRSEGAYV DDKTGKIVQG NLRTALTGLD RAKAEGFSIV YLPPIFPIGV
TNRKGKNNAL TATEDDPGSP FGIGSQLGGH DTVDPLLGTM EDFENFVAYA HKLGLEIALD
FALQCSPDHP WVKQHPEWFK IKPDGTIAFA ENPPKKYQDI YPIDFEKDMR GIEKEVERIL
LLWISKGVTI FRVDNPHTKP VRFWQDVITA VTKKHPEVLF LAEAFTRPAM MRSLSFAGFT
QSHCYFPWRN TSEELNEYLA ETNSNQAFFQ HNTFWPTTPD ILTAYLRDNG IAGHAVRAVL
AALGSPSWGI YNGYELIENV QCGQFEEPND NEKYEIKIRD WQKADDYGIS ELLTRLNQIR
NAHPATRTYH DLHVLPTANP ALVAFYRHLP ANLSENGTAD TVIVVVNLDG HNAQQGSVHI
EPSALGLQGS EIRVHDELTG RNFTWSYDNY VSLAPWADVA HVLTVRQQDA PAPRKSKR
//
