ID W5MBZ8_LEPOC Unreviewed; 609 AA.
AC W5MBZ8;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 50.
DE RecName: Full=glutaminase {ECO:0000256|ARBA:ARBA00012918};
DE EC=3.5.1.2 {ECO:0000256|ARBA:ARBA00012918};
OS Lepisosteus oculatus (Spotted gar).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Holostei; Semionotiformes; Lepisosteidae;
OC Lepisosteus.
OX NCBI_TaxID=7918 {ECO:0000313|Ensembl:ENSLOCP00000005907.1, ECO:0000313|Proteomes:UP000018468};
RN [1] {ECO:0000313|Proteomes:UP000018468}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Di Palma F., Alfoldi J., Johnson J., Berlin A., Gnerre S., Jaffe D.,
RA MacCallum I., Young S., Walker B.J., Lander E.S., Lindblad-Toh K.;
RT "The Draft Genome of Lepisosteus oculatus.";
RL Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSLOCP00000005907.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + L-glutamine = L-glutamate + NH4(+);
CC Xref=Rhea:RHEA:15889, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:29985, ChEBI:CHEBI:58359; EC=3.5.1.2;
CC Evidence={ECO:0000256|ARBA:ARBA00001062};
CC -!- SIMILARITY: Belongs to the glutaminase family.
CC {ECO:0000256|ARBA:ARBA00011076}.
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DR EMBL; AHAT01018728; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AHAT01018729; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_015199717.1; XM_015344231.1.
DR AlphaFoldDB; W5MBZ8; -.
DR STRING; 7918.ENSLOCP00000005907; -.
DR Ensembl; ENSLOCT00000005915.1; ENSLOCP00000005907.1; ENSLOCG00000004907.1.
DR GeneID; 102694598; -.
DR CTD; 27165; -.
DR eggNOG; KOG0506; Eukaryota.
DR GeneTree; ENSGT00390000010463; -.
DR HOGENOM; CLU_016439_1_0_1; -.
DR InParanoid; W5MBZ8; -.
DR OMA; CISARPC; -.
DR OrthoDB; 537490at2759; -.
DR Proteomes; UP000018468; Linkage group LG4.
DR Bgee; ENSLOCG00000004907; Expressed in muscle tissue and 13 other cell types or tissues.
DR GO; GO:0004359; F:glutaminase activity; IBA:GO_Central.
DR GO; GO:0006537; P:glutamate biosynthetic process; IBA:GO_Central.
DR GO; GO:0006543; P:glutamine catabolic process; IBA:GO_Central.
DR Gene3D; 1.10.238.210; -; 1.
DR Gene3D; 1.25.40.20; Ankyrin repeat-containing domain; 1.
DR Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR HAMAP; MF_00313; Glutaminase; 1.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR015868; Glutaminase.
DR InterPro; IPR041541; Glutaminase_EF-hand.
DR NCBIfam; TIGR03814; Gln_ase; 1.
DR PANTHER; PTHR12544; GLUTAMINASE; 1.
DR PANTHER; PTHR12544:SF33; GLUTAMINASE LIVER ISOFORM, MITOCHONDRIAL; 1.
DR Pfam; PF12796; Ank_2; 1.
DR Pfam; PF17959; EF-hand_14; 1.
DR Pfam; PF04960; Glutaminase; 1.
DR SMART; SM00248; ANK; 2.
DR SUPFAM; SSF48403; Ankyrin repeat; 1.
DR SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 1.
PE 3: Inferred from homology;
KW ANK repeat {ECO:0000256|ARBA:ARBA00023043, ECO:0000256|PROSITE-
KW ProRule:PRU00023}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000018468};
KW Repeat {ECO:0000256|ARBA:ARBA00022737}.
FT DOMAIN 81..163
FT /note="Glutaminase EF-hand"
FT /evidence="ECO:0000259|Pfam:PF17959"
FT REPEAT 526..546
FT /note="ANK"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00023"
FT REGION 587..609
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 591..609
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 609 AA; 67716 MW; 7718F80A58A90B58 CRC64;
MHCFRLLRAT HAGVRDVLVR DVFPKGAGAP GICRGSLFRR RRPGRQFCTG SASHLQQNAV
DRDHAPWQRG ASKKGQFLGL EDLLFYTITE GEEQIPVARF IAALKSRGLW TSDPRLRDCM
DLLRQSVQDT VGGSMMDREL FRKCVGSNIV LLTQAFRNKF VIPDFESFTA HINQLYRNAQ
AQTIGKVADY IPQLAKFSPD LWGVSLCTVD GQRHSVGDTK VPFCLQSCVK PLEYAIALHE
AGTERVHRYV GKEPSGLKFN KLFLNEEDKP HNPMVNAGAI VISSLIKPGS NKAEKFDCVM
EFLMKMAGRE FVGFSNATFQ SEKETGDRNF AIGYYLKEKK CFPSGVDMIA ALDFYFQLCS
IEVTCESGSV MAATLANGGI CPITGERVLS AEAVRNTLSL MHSCGMYDFS GQFAFHVGLP
AKSGVSGAVL LVVPNVMGLM CWSPPLDKVG NSVRGIHFCQ ELVSLFNFHN YDNLRHFAKK
LDPRREPGDV RNKSVVNLLF AAYSGDVSAL RRFALSATNM EQKDYDSRTA LHVAAAEGHV
EVVLFLTDTC KVNPFVKDRW GNIPLDDAVQ FGHEAVVKVL KEYQQHYSPR EPNSEAEDRG
KLETLEGMV
//
