ID W5NZ74_SHEEP Unreviewed; 951 AA.
AC W5NZ74;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 62.
DE RecName: Full=hexokinase {ECO:0000256|ARBA:ARBA00012324};
DE EC=2.7.1.1 {ECO:0000256|ARBA:ARBA00012324};
GN Name=HK3 {ECO:0000313|Ensembl:ENSOARP00000003472.1};
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940 {ECO:0000313|Ensembl:ENSOARP00000003472.1, ECO:0000313|Proteomes:UP000002356};
RN [1] {ECO:0000313|Ensembl:ENSOARP00000003472.1, ECO:0000313|Proteomes:UP000002356}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Texel {ECO:0000313|Ensembl:ENSOARP00000003472.1,
RC ECO:0000313|Proteomes:UP000002356};
RX PubMed=20809919; DOI=10.1111/j.1365-2052.2010.02100.x;
RA Archibald A.L., Cockett N.E., Dalrymple B.P., Faraut T., Kijas J.W.,
RA Maddox J.F., McEwan J.C., Hutton Oddy V., Raadsma H.W., Wade C., Wang J.,
RA Wang W., Xun X.;
RT "The sheep genome reference sequence: a work in progress.";
RL Anim. Genet. 41:449-453(2010).
RN [2] {ECO:0000313|Ensembl:ENSOARP00000003472.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-fructose = ADP + D-fructose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:16125, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:37721, ChEBI:CHEBI:61527, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001397};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:16126;
CC Evidence={ECO:0000256|ARBA:ARBA00001397};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000435};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17826;
CC Evidence={ECO:0000256|ARBA:ARBA00000435};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-hexose = ADP + D-hexose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:22740, ChEBI:CHEBI:4194, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61567, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000417};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22741;
CC Evidence={ECO:0000256|ARBA:ARBA00000417};
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC phosphate and glycerone phosphate from D-glucose: step 1/4.
CC {ECO:0000256|ARBA:ARBA00004888}.
CC -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC {ECO:0000256|ARBA:ARBA00005028}.
CC -!- SIMILARITY: Belongs to the hexokinase family.
CC {ECO:0000256|ARBA:ARBA00009225}.
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DR EMBL; AMGL01092426; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01092427; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01092428; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; W5NZ74; -.
DR SMR; W5NZ74; -.
DR STRING; 9940.ENSOARP00000003472; -.
DR PaxDb; 9940-ENSOARP00000003472; -.
DR Ensembl; ENSOART00000003536.1; ENSOARP00000003472.1; ENSOARG00000003252.1.
DR eggNOG; KOG1369; Eukaryota.
DR HOGENOM; CLU_014393_1_0_1; -.
DR OMA; VRPCEVG; -.
DR UniPathway; UPA00109; UER00180.
DR UniPathway; UPA00242; -.
DR Proteomes; UP000002356; Chromosome 5.
DR Bgee; ENSOARG00000003252; Expressed in leukocyte and 51 other cell types or tissues.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005536; F:glucose binding; IEA:InterPro.
DR GO; GO:0004396; F:hexokinase activity; IEA:UniProtKB-EC.
DR GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0019318; P:hexose metabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0001678; P:intracellular glucose homeostasis; IEA:InterPro.
DR CDD; cd00012; NBD_sugar-kinase_HSP70_actin; 2.
DR Gene3D; 3.30.420.40; -; 2.
DR Gene3D; 3.40.367.20; -; 2.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR001312; Hexokinase.
DR InterPro; IPR019807; Hexokinase_BS.
DR InterPro; IPR022673; Hexokinase_C.
DR InterPro; IPR022672; Hexokinase_N.
DR PANTHER; PTHR19443; HEXOKINASE; 1.
DR PANTHER; PTHR19443:SF1; HEXOKINASE-3; 1.
DR Pfam; PF00349; Hexokinase_1; 2.
DR Pfam; PF03727; Hexokinase_2; 2.
DR PRINTS; PR00475; HEXOKINASE.
DR SUPFAM; SSF53067; Actin-like ATPase domain; 4.
DR PROSITE; PS00378; HEXOKINASE_1; 1.
DR PROSITE; PS51748; HEXOKINASE_2; 2.
PE 3: Inferred from homology;
KW Allosteric enzyme {ECO:0000256|ARBA:ARBA00022533};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Glycolysis {ECO:0000256|ARBA:ARBA00023152};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000002356};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 60..257
FT /note="Hexokinase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00349"
FT DOMAIN 266..499
FT /note="Hexokinase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03727"
FT DOMAIN 510..701
FT /note="Hexokinase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00349"
FT DOMAIN 708..941
FT /note="Hexokinase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03727"
FT REGION 1..53
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 951 AA; 102470 MW; 34497C50F15A20F6 CRC64;
EKEELRSKES DQLLLLPGGH PSPSWNHMDS IGSLGLQQGE GAPGCPQEGL PCPSNTSEMV
QECLQQFKVT GAQLRQIQTS LLGSMEQALR GQAGPAPAVR MLPTYVGSIP HGTEQGDFVV
LELGATGASL RVLWVTLTGI EGHKVEPRSQ EFVIPQEVML GPGQQLFDFA ARCLSEFLDV
LPVGNQGLQL GFSFSFPCHQ TGLDKSTLIS WTKGFKCSDV EGQDVVQLLR DAIQRQGAYS
IDVVAVVNDT VGTMMGCEPG VGPCEVGLVV DTGTNACYME EARHVAVLDE DRGRVCISIE
WGSFSDEGAL GPVQTIFDRT LDHESLNPGA QRFEKMIGGL YLGELVRLVL AHLAQRGALF
GGHTSPVLRS QGSILLEHVA EMEDPSAGAA RVHAVLQDLG LNPKASDAEW VQCVCVAVCT
RAAQLCAAAL AAVLSRLQHS REQQALQIAV ATGGRVFERH PRFLSVLRET VMLLAPDCNV
SFIPSVDGGG RGVAMVTAVA ARLAAHRRLL EETLAPFRLT REQLAEVQAQ MREAMAKGLQ
GEASSLRMLP TYVRATPDGS ERGDFLALDL GGTNFRVLLV RVATGGVQIS SQIYSIPECV
AQGSGQQLFD HIVDCIVDFQ QKQGLSGQSL PLGFTFSFPC RQMGLDQGIL LNWTKGFNAS
DCEGQDVVCL LREAIRRRQA VELNVVAIVN DTVGTMMSCG YEDPHCEVGL IVGTGTNACY
MEELRNVASV DGDSGQMCIN TEWGAFGDDG SLSMLSTRFD ACVDQASINP GKQRFEKMIS
GMYLGEIVRH ILLHLTSLGV LFRGQQTQRL QTRDIFKTKF LSEIESDSLA LRQVRAILED
LGLPLTSDDA LMVLEVCQAV SKRAAQLCGA GVAAVVEKIR ENRGLEELTI SVGVDGTLYK
LHPHFSSLVA ATVRELAPRC VVTFLQSEDG SGKGAALVTA VACRLAQRTR V
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