ID W5PZ24_SHEEP Unreviewed; 363 AA.
AC W5PZ24; A0A6P3E2F3;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 52.
DE RecName: Full=S-adenosylmethionine-dependent nucleotide dehydratase RSAD2 {ECO:0000256|ARBA:ARBA00035038};
DE AltName: Full=Radical S-adenosyl methionine domain-containing protein 2 {ECO:0000256|ARBA:ARBA00035040};
DE AltName: Full=Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible {ECO:0000256|ARBA:ARBA00035042};
GN Name=RSAD2 {ECO:0000313|Ensembl:ENSOARP00000015710.1};
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940 {ECO:0000313|Ensembl:ENSOARP00000015710.1, ECO:0000313|Proteomes:UP000002356};
RN [1] {ECO:0000313|Ensembl:ENSOARP00000015710.1, ECO:0000313|Proteomes:UP000002356}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Texel {ECO:0000313|Ensembl:ENSOARP00000015710.1,
RC ECO:0000313|Proteomes:UP000002356};
RX PubMed=20809919; DOI=10.1111/j.1365-2052.2010.02100.x;
RA Archibald A.L., Cockett N.E., Dalrymple B.P., Faraut T., Kijas J.W.,
RA Maddox J.F., McEwan J.C., Hutton Oddy V., Raadsma H.W., Wade C., Wang J.,
RA Wang W., Xun X.;
RT "The sheep genome reference sequence: a work in progress.";
RL Anim. Genet. 41:449-453(2010).
RN [2] {ECO:0000313|Ensembl:ENSOARP00000015710.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=AH2 + CTP + S-adenosyl-L-methionine = 3'-deoxy-3',4'-
CC didehydro-CTP + 5'-deoxyadenosine + A + H(+) + H2O + L-methionine;
CC Xref=Rhea:RHEA:65944, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17319, ChEBI:CHEBI:17499,
CC ChEBI:CHEBI:37563, ChEBI:CHEBI:57844, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:166821; Evidence={ECO:0000256|ARBA:ARBA00034994};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004397}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004397}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004397}. Lipid droplet
CC {ECO:0000256|ARBA:ARBA00004502}. Membrane
CC {ECO:0000256|ARBA:ARBA00004287}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004287}; Cytoplasmic side
CC {ECO:0000256|ARBA:ARBA00004287}. Mitochondrion inner membrane
CC {ECO:0000256|ARBA:ARBA00004273}. Mitochondrion outer membrane
CC {ECO:0000256|ARBA:ARBA00004294}.
CC -!- SIMILARITY: Belongs to the radical SAM superfamily. RSAD2 family.
CC {ECO:0000256|ARBA:ARBA00035008}.
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DR EMBL; AMGL01075211; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_004005718.1; XM_004005669.3.
DR AlphaFoldDB; W5PZ24; -.
DR SMR; W5PZ24; -.
DR STRING; 9940.ENSOARP00000015710; -.
DR PaxDb; 9940-ENSOARP00000015710; -.
DR Ensembl; ENSOART00000015942.1; ENSOARP00000015710.1; ENSOARG00000014648.1.
DR GeneID; 101115013; -.
DR KEGG; oas:101115013; -.
DR CTD; 91543; -.
DR eggNOG; ENOG502QQMH; Eukaryota.
DR HOGENOM; CLU_049058_2_1_1; -.
DR OMA; ERWFKKY; -.
DR OrthoDB; 3671494at2759; -.
DR Proteomes; UP000002356; Chromosome 3.
DR Bgee; ENSOARG00000014648; Expressed in leukocyte and 52 other cell types or tissues.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005811; C:lipid droplet; IEA:UniProtKB-SubCell.
DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR026372; RSAD2.
DR InterPro; IPR007197; rSAM.
DR NCBIfam; TIGR04278; viperin; 1.
DR NCBIfam; NF038283; viperin_w_prok; 1.
DR PANTHER; PTHR21339; RADICAL S-ADENOSYL METHIONINE DOMAIN-CONTAINING PROTEIN 2; 1.
DR PANTHER; PTHR21339:SF0; RADICAL S-ADENOSYL METHIONINE DOMAIN-CONTAINING PROTEIN 2; 1.
DR Pfam; PF13353; Fer4_12; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SFLD; SFLDG01067; SPASM/twitch_domain_containing; 1.
DR SFLD; SFLDF00318; Viperin; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Immunity {ECO:0000256|ARBA:ARBA00022859};
KW Innate immunity {ECO:0000256|ARBA:ARBA00022588};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Lipid droplet {ECO:0000256|ARBA:ARBA00022677};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000002356};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691}.
FT DOMAIN 71..291
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 363 AA; 41577 MW; 2603A89C54D7C2C4 CRC64;
MWMLVPVAFA LKLLSIFLQP LGSVWSSLGP LFLQLRAASW LAGGERSRLL QSRREAGEPS
RAEGEGGGDL PTTPTSVNYH FTRQCNYKCG FCFHTAKTSF VLPLEEAKRG LLMLKEAGME
KINFSGGEPF LQDRGEYLGR LVKFCKQELQ LPSVSIVSNG SLIRESWFQK YGEYLDILAI
SCDSFDEQVN VLIGRGQGKK NHVENLQKLR TWCKEYRVAF KINSVINRFN VDEDMKEQIN
ALNPVRWKVF QCLIIEGENS GEDALREAER FVISDEEFEA FLDRHKDVSC LVPESNQKMR
DSYLILDEYM RFLNCRNGRK DPSKSILDVG VEEAIKFSGF DEKMFLKRGG KYVWSKADLK
LDW
//
