ID W6TEF4_HOLOB Unreviewed; 616 AA.
AC W6TEF4;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE SubName: Full=NADP-dependent malic enzyme {ECO:0000313|EMBL:ETZ07104.1};
GN ORFNames=P618_200733 {ECO:0000313|EMBL:ETZ07104.1};
OS Holospora obtusa F1.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Holosporales; Holosporaceae;
OC Holospora.
OX NCBI_TaxID=1399147 {ECO:0000313|EMBL:ETZ07104.1, ECO:0000313|Proteomes:UP000019112};
RN [1] {ECO:0000313|EMBL:ETZ07104.1, ECO:0000313|Proteomes:UP000019112}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=F1 {ECO:0000313|EMBL:ETZ07104.1,
RC ECO:0000313|Proteomes:UP000019112};
RX PubMed=25115770;
RA Dohra H., Tanaka K., Suzuki T., Fujishima M., Suzuki H.;
RT "Draft genome sequences of three Holospora species (Holospora obtusa,
RT Holospora undulata, and Holospora elegans), endonuclear symbiotic bacteria
RT of the ciliate Paramecium caudatum.";
RL FEMS Microbiol. Lett. 359:16-18(2014).
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ETZ07104.1}.
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DR EMBL; AWTR02000065; ETZ07104.1; -; Genomic_DNA.
DR RefSeq; WP_021826869.1; NZ_AWTR02000065.1.
DR AlphaFoldDB; W6TEF4; -.
DR STRING; 1399147.P618_200733; -.
DR eggNOG; COG0281; Bacteria.
DR Proteomes; UP000019112; Unassembled WGS sequence.
DR GO; GO:0004470; F:malic enzyme activity; IEA:InterPro.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR CDD; cd05311; NAD_bind_2_malic_enz; 1.
DR Gene3D; 3.40.50.10380; Malic enzyme, N-terminal domain; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR046346; Aminoacid_DH-like_N_sf.
DR InterPro; IPR012301; Malic_N_dom.
DR InterPro; IPR037062; Malic_N_dom_sf.
DR InterPro; IPR012302; Malic_NAD-bd.
DR InterPro; IPR045213; Malic_NAD-bd_bact_type.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR43237; NADP-DEPENDENT MALIC ENZYME; 1.
DR PANTHER; PTHR43237:SF4; NADP-DEPENDENT MALIC ENZYME; 1.
DR Pfam; PF00390; malic; 1.
DR Pfam; PF03949; Malic_M; 1.
DR SMART; SM01274; malic; 1.
DR SMART; SM00919; Malic_M; 1.
DR SUPFAM; SSF53223; Aminoacid dehydrogenase-like, N-terminal domain; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 4: Predicted;
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000019112}.
FT DOMAIN 16..149
FT /note="Malic enzyme N-terminal"
FT /evidence="ECO:0000259|SMART:SM01274"
FT DOMAIN 161..396
FT /note="Malic enzyme NAD-binding"
FT /evidence="ECO:0000259|SMART:SM00919"
SQ SEQUENCE 616 AA; 68660 MW; ED72753959BD784E CRC64;
MKFKVPSLHY HAYPKAGKWE MIPSKSLKGW RDFALAYNPG VAFACEAIVD DPHSVYDLTN
KGHLVAVITN GTAVLGMGDI GPLAAKPVME GKSVLIKYFS GLDSIDLELA ETDPYKLIEH
IAALAPSFGA INLEDIKAPE CFIVEQALIE RLTIPVFHDD QHGTAIVVCA AVLNGLYLKN
LSIEEAIVVV NGAGAGALAC LSLLCHFGLK KENIWLCDQK GVVYKGREHV NPYKAEYAQN
TCKRTLSEAL QGADVFLGLS KGRILTKDML SVMSARPLIF ALANPVPEIF PEEVYEAHPD
ALVSTGRSDY PNQINNILCF PYMFKGALAV RASCISQEMK KACVQELENI AREGEWGSCG
AYEGELHSFG SKYFIPKAFD PRLRVRLPMA IAQAAVRSGV AFLEESVLLD FKQQLIWEAY
SWLPTFCRLY LRKSFEISYF SKPFFVFSYD LDAQDRWKLF KSALLALQSH DLCTVKVVHP
KGGLYFDDSD LPHCGQTDLS TVHLHGFSCL PGQDIFKLKS ELHEECCLIS LLKEAKQDTK
QYLTQLMKAA SVLYQSPCEL KDLNSSNMIW SVPLEISLPC DTHIYGETLY IHKNISFRDM
IELAALVMGR VYEHNT
//
