ID   W7J748_9PSEU            Unreviewed;       709 AA.
AC   W7J748;
DT   16-APR-2014, integrated into UniProtKB/TrEMBL.
DT   16-APR-2014, sequence version 1.
DT   27-MAR-2024, entry version 45.
DE   SubName: Full=Glycogen debranching enzyme {ECO:0000313|EMBL:EWC61874.1};
GN   ORFNames=UO65_2807 {ECO:0000313|EMBL:EWC61874.1};
OS   Actinokineospora spheciospongiae.
OC   Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
OC   Pseudonocardiaceae; Actinokineospora.
OX   NCBI_TaxID=909613 {ECO:0000313|EMBL:EWC61874.1, ECO:0000313|Proteomes:UP000019277};
RN   [1] {ECO:0000313|EMBL:EWC61874.1, ECO:0000313|Proteomes:UP000019277}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EG49 {ECO:0000313|EMBL:EWC61874.1,
RC   ECO:0000313|Proteomes:UP000019277};
RX   PubMed=24604655;
RA   Harjes J., Ryu T., Abdelmohsen U.R., Moitinho-Silva L., Horn H., Ravasi T.,
RA   Hentschel U.;
RT   "Draft Genome Sequence of the Antitrypanosomally Active Sponge-Associated
RT   Bacterium Actinokineospora sp. Strain EG49.";
RL   Genome Announc. 2:e00160-14(2014).
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 13 family.
CC       {ECO:0000256|ARBA:ARBA00008061}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EWC61874.1}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AYXG01000100; EWC61874.1; -; Genomic_DNA.
DR   RefSeq; WP_035282533.1; NZ_AYXG01000100.1.
DR   AlphaFoldDB; W7J748; -.
DR   STRING; 909613.UO65_2807; -.
DR   PATRIC; fig|909613.9.peg.2807; -.
DR   eggNOG; COG1523; Bacteria.
DR   OrthoDB; 3236218at2; -.
DR   Proteomes; UP000019277; Unassembled WGS sequence.
DR   GO; GO:0004133; F:glycogen debranching enzyme activity; IEA:InterPro.
DR   GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0005980; P:glycogen catabolic process; IEA:InterPro.
DR   CDD; cd11326; AmyAc_Glg_debranch; 1.
DR   CDD; cd02856; E_set_GDE_Isoamylase_N; 1.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR   InterPro; IPR044505; GlgX_Isoamylase_N_E_set.
DR   InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR   InterPro; IPR004193; Glyco_hydro_13_N.
DR   InterPro; IPR013780; Glyco_hydro_b.
DR   InterPro; IPR011837; Glycogen_debranch_GlgX.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR014756; Ig_E-set.
DR   NCBIfam; TIGR02100; glgX_debranch; 1.
DR   PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR   PANTHER; PTHR43002:SF1; ISOAMYLASE 1, CHLOROPLASTIC; 1.
DR   Pfam; PF00128; Alpha-amylase; 1.
DR   Pfam; PF02922; CBM_48; 1.
DR   SMART; SM00642; Aamy; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE   3: Inferred from homology;
KW   Reference proteome {ECO:0000313|Proteomes:UP000019277}.
FT   DOMAIN          166..572
FT                   /note="Glycosyl hydrolase family 13 catalytic"
FT                   /evidence="ECO:0000259|SMART:SM00642"
FT   REGION          470..492
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        470..485
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   709 AA;  77402 MW;  94253804D36E3553 CRC64;
     MSHTDPRTLP GHPFPLGAHP EAGGVRFAVA SSVAESVELC LVGDGDDAPV EQRIELTERT
     FGIWHALVPG ITPGQRYGYR VHGPWEPGRG IRCNPAKLLV DPYARRITGT VGDLAAVRAH
     RGDALTGAPS RLDSRGSVPL SVVAAAGGSA TGARPDVPFE ETVVYELHVR GFTKLHPEVP
     KHQRGTYLGM AHPAVIEHLV RLGVTAVELL PVHSHSDEPM LLRSGRHNYW GYSTLGFLAP
     HEGYACRPGH EIEEFRTMVA ALHAAGIEVI LDVVYNHTCE GGVDGPTLSM RGLDAPAYYL
     LDDAGRDIDL TGCGNTLDPG SPTVVRLVTD SMRYFATELG VDGFRLDLAS ALGRPRGGAF
     DPNSTLLTAI TTDPVLSNRK LIAEPWDATG EGYRVGGFGG QWAEWNGRFR DGARDFWRGA
     TGPRDLAYRL SGSSDLYAGS GRRPWASINF ITAHDGFTLR DLVSYDHKHN QENGEHNRDG
     TDDNRSWNCG AEGETGDPTV LALRDRQARN LLGTLLLSTG TPMLCAGDER WRTQHGNNNP
     YCLDNHTSWF DWAVDARAED LLAFTRRLTA LRAESPALRQ PHFFDGRPTR SGEPDLVWFR
     ADGEVMTDED WFDEGNRTLG MWIDGSECLS RNREGELVAD DSWLLLLHSG PDAVEFTLPS
     QRYGECFVPV LDSGTPRGVP AAVDPLSPGK AMVLTGRTLL LLRAPRTNQ
//