ID W7MY78_GIBM7 Unreviewed; 830 AA.
AC W7MY78;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 24-JAN-2024, entry version 39.
DE RecName: Full=tRNA ligase {ECO:0000256|PIRNR:PIRNR019634};
DE EC=6.5.1.3 {ECO:0000256|PIRNR:PIRNR019634};
GN ORFNames=FVEG_08903 {ECO:0000313|EMBL:EWG49342.1};
OS Gibberella moniliformis (strain M3125 / FGSC 7600) (Maize ear and stalk rot
OS fungus) (Fusarium verticillioides).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC Fusarium fujikuroi species complex.
OX NCBI_TaxID=334819 {ECO:0000313|EMBL:EWG49342.1, ECO:0000313|Proteomes:UP000009096};
RN [1] {ECO:0000313|EMBL:EWG49342.1, ECO:0000313|Proteomes:UP000009096}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=M3125 / FGSC 7600 {ECO:0000313|Proteomes:UP000009096};
RX PubMed=20237561; DOI=10.1038/nature08850;
RA Ma L.J., van der Does H.C., Borkovich K.A., Coleman J.J., Daboussi M.J.,
RA Di Pietro A., Dufresne M., Freitag M., Grabherr M., Henrissat B.,
RA Houterman P.M., Kang S., Shim W.B., Woloshuk C., Xie X., Xu J.R.,
RA Antoniw J., Baker S.E., Bluhm B.H., Breakspear A., Brown D.W.,
RA Butchko R.A., Chapman S., Coulson R., Coutinho P.M., Danchin E.G.,
RA Diener A., Gale L.R., Gardiner D.M., Goff S., Hammond-Kosack K.E.,
RA Hilburn K., Hua-Van A., Jonkers W., Kazan K., Kodira C.D., Koehrsen M.,
RA Kumar L., Lee Y.H., Li L., Manners J.M., Miranda-Saavedra D., Mukherjee M.,
RA Park G., Park J., Park S.Y., Proctor R.H., Regev A., Ruiz-Roldan M.C.,
RA Sain D., Sakthikumar S., Sykes S., Schwartz D.C., Turgeon B.G.,
RA Wapinski I., Yoder O., Young S., Zeng Q., Zhou S., Galagan J., Cuomo C.A.,
RA Kistler H.C., Rep M.;
RT "Comparative genomics reveals mobile pathogenicity chromosomes in
RT Fusarium.";
RL Nature 464:367-373(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + (ribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC (ribonucleotide)m = (ribonucleotide)n+m + AMP + diphosphate.;
CC EC=6.5.1.3; Evidence={ECO:0000256|PIRNR:PIRNR019634};
CC -!- SIMILARITY: Belongs to the TRL1 family.
CC {ECO:0000256|PIRNR:PIRNR019634}.
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DR EMBL; DS022253; EWG49342.1; -; Genomic_DNA.
DR RefSeq; XP_018755533.1; XM_018897784.1.
DR AlphaFoldDB; W7MY78; -.
DR STRING; 334819.W7MY78; -.
DR GeneID; 30066597; -.
DR KEGG; fvr:FVEG_08903; -.
DR VEuPathDB; FungiDB:FVEG_08903; -.
DR eggNOG; ENOG502QQB9; Eukaryota.
DR OrthoDB; 1405617at2759; -.
DR Proteomes; UP000009096; Chromosome 5.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0051730; F:GTP-dependent polyribonucleotide 5'-hydroxyl-kinase activity; IEA:InterPro.
DR GO; GO:0008081; F:phosphoric diester hydrolase activity; IEA:InterPro.
DR GO; GO:0003972; F:RNA ligase (ATP) activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006388; P:tRNA splicing, via endonucleolytic cleavage and ligation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR019039; T4-Rnl1-like_N.
DR InterPro; IPR012387; Trl1_fun.
DR InterPro; IPR015966; tRNA_lig_kin_fungi.
DR InterPro; IPR015965; tRNA_lig_PDEase.
DR PANTHER; PTHR32004; TRNA LIGASE; 1.
DR PANTHER; PTHR32004:SF1; TRNA LIGASE; 1.
DR Pfam; PF09511; RNA_lig_T4_1; 1.
DR Pfam; PF08302; tRNA_lig_CPD; 1.
DR Pfam; PF08303; tRNA_lig_kinase; 1.
DR PIRSF; PIRSF019634; tRNA_lig_yeast; 1.
PE 3: Inferred from homology;
KW Ligase {ECO:0000256|PIRNR:PIRNR019634};
KW Reference proteome {ECO:0000313|Proteomes:UP000009096};
KW tRNA processing {ECO:0000256|PIRNR:PIRNR019634}.
FT DOMAIN 102..336
FT /note="T4 RNA ligase 1-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF09511"
FT DOMAIN 427..584
FT /note="tRNA ligase kinase"
FT /evidence="ECO:0000259|Pfam:PF08303"
FT DOMAIN 587..827
FT /note="tRNA ligase phosphodiesterase"
FT /evidence="ECO:0000259|Pfam:PF08302"
FT REGION 633..655
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 153
FT /note="N6-AMP-lysine intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR019634-50"
SQ SEQUENCE 830 AA; 94348 MW; 52A93F8A4E00F7F5 CRC64;
MLVYRRSLCS LLTPLSRVTK DHQNLRASSS ALDRMSAPYA PQDVQAVANV VRALDSARKD
KKRGGFAVKK TTFDVKGSAD GIQVDSWRLQ DWDYKRHDLP TYARGLFTTK TRRNEPEIAV
RGYDKFFNVN EVHETKWENI FTRTQGPYEL TLKENGCIIF IAGLEDDTLI VCSKHSTGDR
DDIQVSHASA GEKRLEQQLA AVGKTKADLA RELRKRNVTA VAELCDDQFE EHILAYGPDK
AGLYLHGINL NLPEFATYPS RFVQEFADAW AFRKTGLITM DDIHQVKAFL EEVAETGAHD
GRDVEGFVIR CKMSHDPSKH PYRDWFFKYK FEEPYLMYRQ WRECTKALIA GKQPKFKKHT
KITEEYLLYA RRRLAADPKL GKEYNNNHGI IALRDDFLKF KNINGADAAN MGELDPVVMT
EVTRDVILCP IATIGCGKTT LAMGLSHLFG WGHVQNDNIS GKGRPPRFTK MVLDELKDHP
AVIADRNNAQ RHERKQIITD VKLQHSSAKL VCMNFKHDED SIDEIRRITQ ERIIARGDNH
QTIHAASDKE KFIGVMEGFI NRYEACNPHS RPDDGFDAVI DLDPTASSRQ NLETLVTQLH
KLFPNLVKEI PSAASFDAAM DYALGYKPDF RHDIPDRGKK GYPQQQKPKV QKPRKMEYMS
VGISTQEVNN ALEQAFKATQ PATSRLYTQL KQTRRIQPKF HVTLLHKSGS KEHPELWQRY
AALQKEAEAA GDPEGQVGEC DVMLERAVFD DRIMAIVVRL ADQNDQWQCV NRVAHITVGT
RDDSVKPKES NDLLARWLEV GSSPETKIGE VVFEERPTLK GTVAPVLSKF
//
