ID W7TLI3_9STRA Unreviewed; 719 AA.
AC W7TLI3;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 24-JAN-2024, entry version 24.
DE RecName: Full=glutamine--fructose-6-phosphate transaminase (isomerizing) {ECO:0000256|ARBA:ARBA00012916};
DE EC=2.6.1.16 {ECO:0000256|ARBA:ARBA00012916};
GN ORFNames=Naga_100246g4 {ECO:0000313|EMBL:EWM24368.1};
OS Nannochloropsis gaditana.
OC Eukaryota; Sar; Stramenopiles; Ochrophyta; Eustigmatophyceae;
OC Eustigmatales; Monodopsidaceae; Nannochloropsis.
OX NCBI_TaxID=72520 {ECO:0000313|EMBL:EWM24368.1, ECO:0000313|Proteomes:UP000019335};
RN [1] {ECO:0000313|EMBL:EWM24368.1, ECO:0000313|Proteomes:UP000019335}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=B-31 {ECO:0000313|EMBL:EWM24368.1,
RC ECO:0000313|Proteomes:UP000019335};
RX PubMed=23966634; DOI=10.1093/mp/sst120;
RA Corteggiani Carpinelli E., Telatin A., Vitulo N., Forcato C., D'Angelo M.,
RA Schiavon R., Vezzi A., Giacometti G.M., Morosinotto T., Valle G.;
RT "Chromosome Scale Genome Assembly and Transcriptome Profiling of
RT Nannochloropsis gaditana in Nitrogen Depletion.";
RL Mol. Plant 7:323-335(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-
CC phosphate + L-glutamate; Xref=Rhea:RHEA:13237, ChEBI:CHEBI:29985,
CC ChEBI:CHEBI:58359, ChEBI:CHEBI:58725, ChEBI:CHEBI:61527; EC=2.6.1.16;
CC Evidence={ECO:0000256|ARBA:ARBA00001031};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EWM24368.1}.
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DR EMBL; AZIL01001300; EWM24368.1; -; Genomic_DNA.
DR AlphaFoldDB; W7TLI3; -.
DR EnsemblProtists; EWM24368; EWM24368; Naga_100246g4.
DR OrthoDB; 1705390at2759; -.
DR Proteomes; UP000019335; Chromosome 14.
DR GO; GO:0097367; F:carbohydrate derivative binding; IEA:InterPro.
DR GO; GO:0004360; F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity; IEA:UniProtKB-EC.
DR GO; GO:1901137; P:carbohydrate derivative biosynthetic process; IEA:InterPro.
DR GO; GO:0006541; P:glutamine metabolic process; IEA:UniProtKB-KW.
DR CDD; cd00714; GFAT; 1.
DR CDD; cd05008; SIS_GlmS_GlmD_1; 1.
DR CDD; cd05009; SIS_GlmS_GlmD_2; 1.
DR Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR InterPro; IPR017932; GATase_2_dom.
DR InterPro; IPR005855; GFAT.
DR InterPro; IPR047084; GFAT_N.
DR InterPro; IPR035466; GlmS/AgaS_SIS.
DR InterPro; IPR035490; GlmS/FrlB_SIS.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR InterPro; IPR001347; SIS_dom.
DR InterPro; IPR046348; SIS_dom_sf.
DR NCBIfam; TIGR01135; glmS; 1.
DR PANTHER; PTHR10937; GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING; 1.
DR PANTHER; PTHR10937:SF0; GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING); 1.
DR Pfam; PF13522; GATase_6; 1.
DR Pfam; PF01380; SIS; 2.
DR SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
DR SUPFAM; SSF53697; SIS domain; 1.
DR PROSITE; PS51278; GATASE_TYPE_2; 1.
DR PROSITE; PS51464; SIS; 2.
PE 4: Predicted;
KW Aminotransferase {ECO:0000256|ARBA:ARBA00022576};
KW Glutamine amidotransferase {ECO:0000256|ARBA:ARBA00022962};
KW Reference proteome {ECO:0000313|Proteomes:UP000019335};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 101..326
FT /note="Glutamine amidotransferase type-2"
FT /evidence="ECO:0000259|PROSITE:PS51278"
FT DOMAIN 388..527
FT /note="SIS"
FT /evidence="ECO:0000259|PROSITE:PS51464"
FT DOMAIN 564..709
FT /note="SIS"
FT /evidence="ECO:0000259|PROSITE:PS51464"
SQ SEQUENCE 719 AA; 77533 MW; BCD9803352F63AE5 CRC64;
MHAQVSSVLV RRGSRHAWAS TVIKMTSSFS GNFAQKNSSR QRYPQQNVVG RYASRAAYMS
PGSSSSSSSS SSFMRWGWGL AAATAALGAL SERQDSPTEA CGIVGVIGED DARSILMEGL
TVLQNRGYDS AGIATISPHD NVLTVTKYAS AGSTYDCIDL VKENSHRHKG HHVGIAHTRW
ATHGGKTDLN AHPHTDSKGR VAVVHNGTIT NSHELRHELQ HAHGIKFVSE TDTEIIAQLI
GLGLDENLSL KDATAEALKR CDGTWGLVVL APKEPDELVV ACNGSPMVLG IGEGRVFIAS
ETSAFNRHTK NFIALKDGEL GVVRADGSTL DLTRVQKAPD VNILLSPAPY PHWTIRECME
QPEAIARALG FGGRMSDTRV YLGGLDRQKE RMQSIKHLVL TGCGTSLHAA MYAAKLMRDF
EAFRTVMAVD AAEVRLSDLP KHHAGLLAIS QSGETKDVHR AVVLADRMGL PTMSVVNTVG
SLIARTTKLG VYLNAGRESS VASTKAFTTQ VTVLTLITAW FRQVREEAGE EALPGRVTEL
LEALQRMPIG FGMARRTREQ CAELAVRLKD KEHVFLLGKG YGEPIALEGA LKLKEMAYIH
AEGYSGGALK HGPFALIEGK EGKFGATPII CLILDDEHAQ HMRTAAEEVK ARGADLIVIT
DNPKLARGLV PDPIIVPNNG PLTALIATLP LQLLAYELAV LRGINPDKPR NLAKAVTVD
//
