ID W9BFA6_KLEPN Unreviewed; 236 AA.
AC W9BFA6;
DT 14-MAY-2014, integrated into UniProtKB/TrEMBL.
DT 14-MAY-2014, sequence version 1.
DT 27-MAR-2024, entry version 77.
DE RecName: Full=Ureidoacrylate amidohydrolase RutB {ECO:0000256|HAMAP-Rule:MF_00830};
DE EC=3.5.1.110 {ECO:0000256|HAMAP-Rule:MF_00830};
GN Name=rutB_2 {ECO:0000313|EMBL:SYR34800.1};
GN Synonyms=rutB {ECO:0000256|HAMAP-Rule:MF_00830,
GN ECO:0000313|EMBL:NGN72374.1}, rutB_1 {ECO:0000313|EMBL:CAH1470255.1};
GN ORFNames=B5L96_06225 {ECO:0000313|EMBL:OVF75890.1}, FXN67_17985
GN {ECO:0000313|EMBL:TYL76824.1}, G4V31_09515
GN {ECO:0000313|EMBL:NGN72374.1}, NCTC11679_03578
GN {ECO:0000313|EMBL:STV65995.1}, SAMEA3499901_03932
GN {ECO:0000313|EMBL:SXN32885.1}, SAMEA3538828_01509
GN {ECO:0000313|EMBL:SYR34800.1}, SAMEA4370542_02856
GN {ECO:0000313|EMBL:SLS49692.1}, VKR_03238
GN {ECO:0000313|EMBL:CAH1470255.1};
OS Klebsiella pneumoniae.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX NCBI_TaxID=573 {ECO:0000313|EMBL:SYR34800.1, ECO:0000313|Proteomes:UP000258253};
RN [1] {ECO:0000313|EMBL:OVF75890.1, ECO:0000313|Proteomes:UP000196447}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=39383 {ECO:0000313|EMBL:OVF75890.1,
RC ECO:0000313|Proteomes:UP000196447};
RA Fouts D., Stalin M.J., Chen L., Wright M., Sutton G., Nguyen K.,
RA Vanduin D., Rojas L., Hujer A., Hujer K., Bonomo R., Kreiswirth B.,
RA Adams M.;
RL Submitted (MAR-2017) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:STV65995.1, ECO:0000313|Proteomes:UP000255239}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC11679 {ECO:0000313|EMBL:STV65995.1,
RC ECO:0000313|Proteomes:UP000255239};
RG Pathogen Informatics;
RA Doyle S.;
RL Submitted (JUN-2018) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|Proteomes:UP000258253, ECO:0000313|Proteomes:UP000259975}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=EuSCAPE_AT029 {ECO:0000313|EMBL:SXN32885.1,
RC ECO:0000313|Proteomes:UP000259975}, EuSCAPE_HU047
RC {ECO:0000313|EMBL:SYR34800.1, ECO:0000313|Proteomes:UP000258253}, and
RC VRES1272 {ECO:0000313|EMBL:SLS49692.1,
RC ECO:0000313|Proteomes:UP000191996};
RG Pathogen Informatics;
RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:TYL76824.1, ECO:0000313|Proteomes:UP000322977}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UCO-494 {ECO:0000313|EMBL:TYL76824.1,
RC ECO:0000313|Proteomes:UP000322977};
RA Morales-Leon F., Caro C., Opazo-Capurro A., Lincopan N.,
RA Dominguez-Yevenes M., Lima C., Bello-Toledo H., Gonzalez-Rocha G.;
RT "Phenotypic and genetic characterization of extended-spectrum b-lactamase-
RT producing hypermucoviscous Klebsiella pneumoniae from Chile.";
RL Submitted (AUG-2019) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:NGN72374.1, ECO:0000313|Proteomes:UP000479475}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=2429 {ECO:0000313|EMBL:NGN72374.1,
RC ECO:0000313|Proteomes:UP000479475};
RA Starkova P.S., Sulyan O.S., Likholetova D.V., Ageevets V.A., Lazareva I.V.,
RA Sopova J.V., Sidorenko S.V.;
RT "Klebsiella pneumoniae genome sequencing and assembly.";
RL Submitted (FEB-2020) to the EMBL/GenBank/DDBJ databases.
RN [6] {ECO:0000313|EMBL:CAH1470255.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=BB1542 {ECO:0000313|EMBL:CAH1470255.1};
RA Delgado-Blas J.;
RL Submitted (JAN-2022) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Hydrolyzes ureidoacrylate to form aminoacrylate and
CC carbamate. The carbamate hydrolyzes spontaneously, thereby releasing
CC one of the nitrogen atoms of the pyrimidine ring as ammonia and one of
CC its carbon atoms as CO2. {ECO:0000256|HAMAP-Rule:MF_00830}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(Z)-2-methylureidoacrylate + H(+) + H2O = (Z)-2-
CC methylaminoacrylate + CO2 + NH4(+); Xref=Rhea:RHEA:42620,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:28938, ChEBI:CHEBI:143783, ChEBI:CHEBI:145735;
CC EC=3.5.1.110; Evidence={ECO:0000256|HAMAP-Rule:MF_00830};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(Z)-3-ureidoacrylate + H(+) + H2O = (Z)-3-aminoacrylate + CO2
CC + NH4(+); Xref=Rhea:RHEA:42624, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:28938, ChEBI:CHEBI:59891,
CC ChEBI:CHEBI:59894; EC=3.5.1.110; Evidence={ECO:0000256|HAMAP-
CC Rule:MF_00830};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(Z)-3-ureidoacrylate + H2O = (Z)-3-aminoacrylate + carbamate +
CC H(+); Xref=Rhea:RHEA:31603, ChEBI:CHEBI:13941, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:59891, ChEBI:CHEBI:59894;
CC Evidence={ECO:0000256|HAMAP-Rule:MF_00830};
CC -!- SIMILARITY: Belongs to the isochorismatase family. RutB subfamily.
CC {ECO:0000256|HAMAP-Rule:MF_00830}.
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DR EMBL; OV753987; CAH1470255.1; -; Genomic_DNA.
DR EMBL; JAAKYD010000007; NGN72374.1; -; Genomic_DNA.
DR EMBL; NDBK01000035; OVF75890.1; -; Genomic_DNA.
DR EMBL; FWJM01000008; SLS49692.1; -; Genomic_DNA.
DR EMBL; UGMG01000001; STV65995.1; -; Genomic_DNA.
DR EMBL; UKGE01000016; SXN32885.1; -; Genomic_DNA.
DR EMBL; ULCI01000005; SYR34800.1; -; Genomic_DNA.
DR EMBL; VSSY01000016; TYL76824.1; -; Genomic_DNA.
DR RefSeq; WP_004224061.1; NZ_WVUB01000002.1.
DR KEGG; kpnk:BN49_2126; -.
DR KEGG; kpx:PMK1_03377; -.
DR Proteomes; UP000191996; Unassembled WGS sequence.
DR Proteomes; UP000196447; Unassembled WGS sequence.
DR Proteomes; UP000255239; Unassembled WGS sequence.
DR Proteomes; UP000258253; Unassembled WGS sequence.
DR Proteomes; UP000259975; Unassembled WGS sequence.
DR Proteomes; UP000322977; Unassembled WGS sequence.
DR Proteomes; UP000479475; Unassembled WGS sequence.
DR Proteomes; UP001158595; Chromosome.
DR GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:UniProtKB-UniRule.
DR GO; GO:0019740; P:nitrogen utilization; IEA:UniProtKB-UniRule.
DR GO; GO:0006212; P:uracil catabolic process; IEA:UniProtKB-UniRule.
DR CDD; cd00431; cysteine_hydrolases; 1.
DR Gene3D; 3.40.50.850; Isochorismatase-like; 1.
DR HAMAP; MF_00830; RutB; 1.
DR InterPro; IPR000868; Isochorismatase-like.
DR InterPro; IPR036380; Isochorismatase-like_sf.
DR InterPro; IPR019916; RutB.
DR NCBIfam; TIGR03614; RutB; 1.
DR PANTHER; PTHR43540:SF6; ISOCHORISMATASE DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR43540; PEROXYUREIDOACRYLATE/UREIDOACRYLATE AMIDOHYDROLASE-RELATED; 1.
DR Pfam; PF00857; Isochorismatase; 1.
DR SUPFAM; SSF52499; Isochorismatase-like hydrolases; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00830}.
FT DOMAIN 18..215
FT /note="Isochorismatase-like"
FT /evidence="ECO:0000259|Pfam:PF00857"
FT ACT_SITE 24
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00830"
FT ACT_SITE 133
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00830"
FT ACT_SITE 166
FT /note="Nucleophile"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00830"
SQ SEQUENCE 236 AA; 25861 MW; 2AB8BA9E31F70A12 CRC64;
MITLPARPES LTFAPQQSAL IVVDMQNAYA SQGGYLDLAG FDVSATRPVI DNINTAVAAA
RAAGMLIIWF QNGWDDQYVE AGGPGSPNYH KSNALKTMRQ RPELQGKLLA KGGWDYQLVD
ELTPQEGDIV LPKPRYSGFF NTPLDSILRS RGIRHLVFTG IATNVCVEST LRDGFFLEYF
GIVLEDATHQ AGPAFAQQAA LFNIETFFGW VSDVESFCHA LSPATPLALA KEKRYA
//
