ID W9G746_9MICO Unreviewed; 557 AA.
AC W9G746;
DT 14-MAY-2014, integrated into UniProtKB/TrEMBL.
DT 14-MAY-2014, sequence version 1.
DT 27-MAR-2024, entry version 28.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=N865_21195 {ECO:0000313|EMBL:EWS99698.1};
OS Intrasporangium oryzae NRRL B-24470.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Intrasporangiaceae;
OC Intrasporangium.
OX NCBI_TaxID=1386089 {ECO:0000313|EMBL:EWS99698.1, ECO:0000313|Proteomes:UP000019489};
RN [1] {ECO:0000313|EMBL:EWS99698.1, ECO:0000313|Proteomes:UP000019489}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NRRL B-24470 {ECO:0000313|EMBL:EWS99698.1,
RC ECO:0000313|Proteomes:UP000019489};
RA Liu H., Wang G.;
RT "Intrasporangium oryzae NRRL B-24470.";
RL Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EWS99698.1}.
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DR EMBL; AWSA01000078; EWS99698.1; -; Genomic_DNA.
DR AlphaFoldDB; W9G746; -.
DR STRING; 1386089.N865_21195; -.
DR PATRIC; fig|1386089.3.peg.4080; -.
DR eggNOG; COG0827; Bacteria.
DR Proteomes; UP000019489; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR33841; DNA METHYLTRANSFERASE YEEA-RELATED; 1.
DR PANTHER; PTHR33841:SF5; TYPE II METHYLTRANSFERASE M.HINDII; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:EWS99698.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000019489};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:EWS99698.1}.
FT DOMAIN 12..239
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 557 AA; 60653 MW; 035E45BA06AED478 CRC64;
MSAEQIEDTA ALRKARGAFF TPEEIARFIS EWAVRSVSDK VLEPSCGEAA FLLAAAERLD
RLTAETGQEF SGHLDGVELH AASAVSAESH VRMAGYETTI QTGDFFTVEP TSTYDAVIGN
PPYVRYQDFS GESRRLSRAA ALRAGVSLTA LASSWASFTL HSAMFLRKGG RLGLVLPAEL
LSVNYAAGVR QFLMERFTRV DLVLFEERVF PGVLEEVVLL MADGFDDGGT DHCFVYQARS
AADLEELLVA RRWTPVNSAD KWTPSLLPHA AFDAYTTLVS GDGFTDLEEW GDTTLGMVTG
NNHYFALTDA RVAELGIPRA ELLPLSPPGS THLRGLTFTT SAHEEMLRGG SAGWLFRPSN
GEPSEAALAY IAAGDATGVS EAYKCRVRKP WWRVPLVPPA DLLLTYMNAD TPRLTTNRAG
VHNLNSVHGI YLKTQHKTLG RDLLPLASLN SVTLLGAETV GRAYGGGMLK IEPREADRLP
VPSAPTVMAA KKALSRLRPK VTRHLRSGRL LEATHLVDEV LLVGELGVRV TELREIREAR
AELTARRTAR GIKQGGL
//
