ID X0H5V4_FUSOX Unreviewed; 2361 AA.
AC X0H5V4;
DT 14-MAY-2014, integrated into UniProtKB/TrEMBL.
DT 14-MAY-2014, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=alpha-1,3-glucan synthase {ECO:0000256|ARBA:ARBA00012688};
DE EC=2.4.1.183 {ECO:0000256|ARBA:ARBA00012688};
GN ORFNames=FOPG_16561 {ECO:0000313|EMBL:EXL67316.1};
OS Fusarium oxysporum f. sp. conglutinans race 2 54008.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC Fusarium oxysporum species complex.
OX NCBI_TaxID=1089457 {ECO:0000313|EMBL:EXL67316.1};
RN [1] {ECO:0000313|EMBL:EXL67316.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=54008 {ECO:0000313|EMBL:EXL67316.1};
RG The Broad Institute Genome Sequencing Platform;
RA Ma L.-J., Gale L.R., Schwartz D.C., Zhou S., Corby-Kistler H., Young S.K.,
RA Zeng Q., Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Alvarado L.,
RA Arachchi H.M., Berlin A., Brown A., Chapman S.B., Chen Z., Dunbar C.,
RA Freedman E., Gearin G., Goldberg J., Griggs A., Gujja S., Heiman D.,
RA Howarth C., Larson L., Lui A., MacDonald P.J.P., Montmayeur A., Murphy C.,
RA Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T., Shenoy N.,
RA Sisk P., Stolte C., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Fusarium oxysporum PHW808.";
RL Submitted (NOV-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EXL67316.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=54008 {ECO:0000313|EMBL:EXL67316.1};
RG The Broad Institute Genomics Platform;
RA Ma L.-J., Corby-Kistler H., Broz K., Gale L.R., Jonkers W., O'Donnell K.,
RA Ploetz R., Steinberg C., Schwartz D.C., VanEtten H., Zhou S., Young S.K.,
RA Zeng Q., Gargeya S., Fitzgerald M., Abouelleil A., Alvarado L.,
RA Chapman S.B., Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M.,
RA Howarth C., Imamovic A., Ireland A., Larimer J., McCowan C., Murphy C.,
RA Pearson M., Poon T.W., Priest M., Roberts A., Saif S., Shea T., Sykes S.,
RA Wortman J., Nusbaum C., Birren B.;
RT "The Genome Annotation of Fusarium oxysporum PHW808.";
RL Submitted (MAY-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[(1->3)-alpha-D-glucosyl](n) + UDP-alpha-D-glucose = [(1->3)-
CC alpha-D-glucosyl](n+1) + H(+) + UDP; Xref=Rhea:RHEA:19749, Rhea:RHEA-
CC COMP:11150, Rhea:RHEA-COMP:11151, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:28100, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885;
CC EC=2.4.1.183; Evidence={ECO:0000256|ARBA:ARBA00000687};
CC -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC {ECO:0000256|ARBA:ARBA00006122}.
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DR EMBL; JH658968; EXL67316.1; -; Genomic_DNA.
DR HOGENOM; CLU_000488_0_0_1; -.
DR Proteomes; UP000030676; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0047657; F:alpha-1,3-glucan synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11323; AmyAc_AGS; 1.
DR CDD; cd03791; GT5_Glycogen_synthase_DULL1-like; 1.
DR Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 2.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR001296; Glyco_trans_1.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013534; Starch_synth_cat_dom.
DR PANTHER; PTHR47182; CELL WALL ALPHA-1,3-GLUCAN SYNTHASE AGS1-RELATED; 1.
DR PANTHER; PTHR47182:SF2; CELL WALL ALPHA-1,3-GLUCAN SYNTHASE MOK13; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF08323; Glyco_transf_5; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Membrane {ECO:0000256|SAM:Phobius}; Signal {ECO:0000256|SAM:SignalP};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT SIGNAL 1..18
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 19..2361
FT /note="alpha-1,3-glucan synthase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004940498"
FT TRANSMEM 1062..1088
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1938..1958
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1970..1989
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2001..2020
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2032..2050
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2062..2082
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2106..2127
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2148..2165
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2185..2206
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2232..2254
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2260..2280
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2287..2309
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 2334..2353
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 64..523
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
FT REGION 1385..1413
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1873..1911
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1390..1413
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1897..1911
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2361 AA; 264394 MW; 3296B049E52701B8 CRC64;
MAPMKLLFSF LLATLAQGLH YDPSEIDYNL NTNKQAKSPL EYSGARKNHD YWPSPANWRM
PFYTLFLDRY ANGDPSNDDI NGTVYEQDIT SNQLRHGGDL QGLVDSLDYI QGMGVKAIYI
AGCPFINFPW GVDSYSPLDF TVLDHHFGAI MDWQNMVDEA HSRGMYVILD HTFSTMGDLL
AFKGFENNTA IFSTKEHDVL YKTGRQYLDF APSNDYNQTC DYPRFYLDTG YPVGTNVTDQ
LVGCFNSDFD QYGDIEAFGV HPDWQRSLAK FASVQDRLRE WVPSVRERLE RFSCITIQQL
DIDGFRFDKA TQITVDALGN HSAAVRDCAS RLGKKNFFLP GEITGGNNYG SIYLGRGRTA
DKYLDDVATA MNLTTKKKDE LANSTTRDLG QNALDAAAFH YSVYRFLTRF LGMSGNLEAG
FDLPLNWVDM WSQMVLTNDL VNANTGKFDP RHMYGTSNQD VFRWPSIAQG IERLLMGFFI
TTLHMPGLPL LLYGEEQAFY IIDSTADNYI FGRQAFTASQ GWRMHGCYTG TSTQYVNWPI
EAARHGCTDE MAAYDHRDPS HPVRNILKSM YAMRENFDTF SEAWLLQPIG NQTFHTILSG
SNTTGTEFGI WSVARAFQPT VQGEFASSPV WLVYHNQNVS TTYRFDCEDE DKAFYAPFDQ
KSTVKNLFYP WDKITLGTST KSWHFSGSSK NSGCISEITM APFEFRAYVP KSDWKGPPPM
ITKFTPGHDV PLLSSSVNGE VDITFEFSKV MDCDGLNEAI SITSVTENGN NVTVTRAKCK
TLASDKPVAS YAGSIPSKFR YSATLTNVDD GVHRITIKNA STKADHEAMN SVDHFLLRVG
AADNPIVFPT SANYSKTLVI NDSNKLFVNH SASGANKWRF STNWGSTWSN WTAYTGGIRE
ITRLPWSGTS LQSWSGDHVM VQYWSQLHSS SSFMQAGDSS NQFERRFPHL FVEGTFNKFG
FDAGVANQMS QISDGVWQMH LMDEWPTQFQ LNVWGMNPDG KPDASWVYGD VDFDGILDRM
PPTSLALNVL NATNPPPLPY LSYQLLFNDA TLTFTKQPQG SMWIQIIMFI LLAVLPVVGG
LTAVWIFLGS FYKVKVNKVG FKKQGLNPFG KLANRLSSVS FENFRHGELG AVPLKRRKVL
IATIEYNIDD WNIKIKIGGL GVMAQLMGKA LKHQDLIWVV PCVGGVEYPV DQRAEPMYIL
SIGKMYEIDV QYHQVQNITY VLLDAPIFRQ QTKSDPYPPR MDDINSAIYY SAWNYCIAEV
IRRFDPTLYH INDYHGAAAP LHLLPERTIP CILSLHNAEF QGLWPMRTAE GFKEVCEVFD
LDPKIVKEYV QFGSVFNLLH AGASYLRVHQ KGFGAVGVSK KYGDRSYARY PIFWGLSKVG
QLPNPDPSDM AEWTRDEEAQ EKDADVNMET ESKRGDLRKQ AQEWAGLEIN PNAELFVFVG
RWSIQKGVDL IADIFPSILE KHKDVQLICI GPVIDLYGKF AALKLAKLME KYPKRVYSKP
EFTALPPYIF SGAEFALIPS RDEPFGLVAV EFGRQGALGV GARVGGLGQM PGFWYTIEST
APQHLLQQFC EAITRALESK AKMRQKMRAW SAKQRFPVAQ WVEDVDKLQS MAIRIHNREA
KKRRRITSGS LLSVPFNVVL HAPGGSRPSL DDSGIEAPLH SAHQSHTSNL SIPNIIETDY
DVEASRRAIS NPLINTAHLA AAPTLGTPGA PPISATTDSA DEPALPRNPM FLNQSASCSV
SSLATITNEP GSRLGVDTFA MHMMSPDESE TRPQAFGLHS PQSPPDASLN FQHDDQWRSS
RLSVENVIGE RNDLKLQIVQ PFFTDVTGEY YKAFEQKLVG LTSKNSDTDL CIEDYLKESE
KEWFKHFHNA KLGRSRSPSR TRSPAPSMSG ETLAPLDDDM QHGEWQNRPD SDDEFLLGTG
YKPPKGFKKW LSIRIGDWPI YSFILAIGQI MASNSYQIVL LAGEASQTAA QLYIIAGTYG
VTSILWWLTF RRLSTVYTLS LPWLFYGFAF VLLGVAPFML ENDRGRLHDT ATAMYAAGAS
SGSMFFAMNF GDEAGAPTSV WIFRAAIIQG IQQLYVVGLW YWGSLISSKT SLVNGMVTSK
TGPKTLVITI PIAVILWFLG VVSFVGLPDC YRQSPDKIPS FYMSLLRRHI VPWFLFSVAG
QSYWLSAPYG RNWQFLFSAK AVTGWAVMLL ILGFFVILWL IGLWIFSMFS KTHPWIVPLF
AIGLGVPRWA QMLWGISGIG LYLPWVGGAV ASAIVSRCLW LWLGVLDTIQ GVGLGMVLLL
TLTRQHVAAT LIGAQILGSG FIILARATAP DKIGPGPVFP DLSEGIMPGI AQPWFWVALC
LQLILPIGFF KFFRKEQVAK P
//
