ID Y3240_PSEAE Reviewed; 285 AA.
AC Q9HZ00;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 27-MAR-2024, entry version 112.
DE RecName: Full=Putative quercetin 2,3-dioxygenase PA3240;
DE Short=Putative quercetinase;
DE EC=1.13.11.24;
DE AltName: Full=Pirin-like protein PA3240;
GN OrderedLocusNames=PA3240;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Putative quercetin 2,3-dioxygenase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=O2 + quercetin = 2-(3,4-dihydroxybenzoyloxy)-4,6-
CC dihydroxybenzoate + CO; Xref=Rhea:RHEA:15381, ChEBI:CHEBI:15379,
CC ChEBI:CHEBI:17245, ChEBI:CHEBI:57628, ChEBI:CHEBI:57694;
CC EC=1.13.11.24;
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000250};
CC Note=Binds 1 divalent metal cation. {ECO:0000250};
CC -!- PATHWAY: Flavonoid metabolism; quercetin degradation.
CC -!- SIMILARITY: Belongs to the pirin family. {ECO:0000305}.
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DR EMBL; AE004091; AAG06628.1; -; Genomic_DNA.
DR PIR; B83239; B83239.
DR RefSeq; NP_251930.1; NC_002516.2.
DR RefSeq; WP_003114805.1; NZ_QZGE01000019.1.
DR AlphaFoldDB; Q9HZ00; -.
DR SMR; Q9HZ00; -.
DR STRING; 208964.PA3240; -.
DR PaxDb; 208964-PA3240; -.
DR DNASU; 882403; -.
DR GeneID; 882403; -.
DR KEGG; pae:PA3240; -.
DR PATRIC; fig|208964.12.peg.3387; -.
DR PseudoCAP; PA3240; -.
DR HOGENOM; CLU_045717_5_0_6; -.
DR InParanoid; Q9HZ00; -.
DR OrthoDB; 9780903at2; -.
DR PhylomeDB; Q9HZ00; -.
DR BioCyc; PAER208964:G1FZ6-3299-MONOMER; -.
DR UniPathway; UPA00724; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0008127; F:quercetin 2,3-dioxygenase activity; IEA:UniProtKB-EC.
DR CDD; cd02247; cupin_pirin_C; 1.
DR CDD; cd02909; cupin_pirin_N; 1.
DR Gene3D; 2.60.120.10; Jelly Rolls; 2.
DR InterPro; IPR012093; Pirin.
DR InterPro; IPR008778; Pirin_C_dom.
DR InterPro; IPR003829; Pirin_N_dom.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR PANTHER; PTHR13903:SF8; PIRIN; 1.
DR PANTHER; PTHR13903; PIRIN-RELATED; 1.
DR Pfam; PF02678; Pirin; 1.
DR Pfam; PF05726; Pirin_C; 1.
DR PIRSF; PIRSF006232; Pirin; 1.
DR SUPFAM; SSF51182; RmlC-like cupins; 1.
PE 3: Inferred from homology;
KW Dioxygenase; Metal-binding; Oxidoreductase; Reference proteome.
FT CHAIN 1..285
FT /note="Putative quercetin 2,3-dioxygenase PA3240"
FT /id="PRO_0000214071"
FT BINDING 60
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000250"
FT BINDING 62
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000250"
FT BINDING 104
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000250"
FT BINDING 106
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /evidence="ECO:0000250"
SQ SEQUENCE 285 AA; 31628 MW; A26ED494A03662D9 CRC64;
MTRYRDVLEL HTGHPASDGA GVRLTRVIGG PSPERFDPFL MLDQFDTQNP DDYVAGFPSH
PHRGFETVTY MLEGRMRHED HLGNRGLLKP GGVQWMTAAH GIIHSEMPEQ VEGAMRGFQL
WVNLPAKNKL APAGYRDIEP EDVPRLETAG GVKVTVIAGR FDDGQAQQIG AVERPDTEPH
YYDLQLPAGG RIAPRLPDGH RVLLYVYEGS LTVEGERPVE IATNRLARLS EEGELSLRSE
AGARVLVLAG KPLHEPIVQY GPFVMNSREE IEQALRDYRD GVLAV
//
