| Entry |
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| Name |
sterol 24-C-methyltransferase;
Delta24-methyltransferase;
Delta24-sterol methyltransferase;
zymosterol-24-methyltransferase;
S-adenosyl-4-methionine:sterol Delta24-methyltransferase;
SMT1;
24-sterol C-methyltransferase;
S-adenosyl-L-methionine:Delta24(23)-sterol methyltransferase;
phytosterol methyltransferase
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| Class |
Transferases;
Transferring one-carbon groups;
Methyltransferases
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| Sysname |
S-adenosyl-L-methionine:zymosterol 24-C-methyltransferase
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| Reaction(IUBMB) |
S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol [RN: R04427]
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| Reaction(KEGG) |
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| Substrate |
S-adenosyl-L-methionine [CPD: C00019];
5alpha-cholesta-8,24-dien-3beta-ol [CPD: C05437]
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| Product |
S-adenosyl-L-homocysteine [CPD: C00021];
24-methylene-5alpha-cholest-8-en-3beta-ol [CPD: C04525]
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| Comment |
Requires glutathione. Acts on a range of sterols with a 24(25)-double bond in the sidechain. While zymosterol is the preferred substrate it also acts on desmosterol, 5alpha-cholesta-7,24-dien-3beta-ol, 5alpha-cholesta-5,7,24-trien-3beta-ol, 4alpha-methylzymosterol and others. S-Adenosyl-L-methionine attacks the Si-face of the 24(25) double bond and the C-24 hydrogen is transferred to C-25 on the Re face of the double bond.
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| History |
EC 2.1.1.41 created 1972, modified 2001
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| Pathway |
| ec01110 | Biosynthesis of secondary metabolites |
| ec01130 | Biosynthesis of antibiotics |
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| Orthology |
| K00559 | sterol 24-C-methyltransferase |
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| Genes |
» show all
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| Reference |
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| Authors |
Moore JT, Gaylor JL. |
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| Title |
Isolation and purification of an S-adenosylmethionine: delta 24-sterol methyltransferase from yeast. |
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| Journal |
J Biol Chem 244:6334-40 (1969) |
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| Reference |
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| Authors |
Venkatramesh M, Guo DA, Jia Z, Nes WD. |
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| Title |
Mechanism and structural requirements for transformation of substrates by the (S)-adenosyl-L-methionine:delta 24(25)-sterol methyl transferase from Saccharomyces cerevisiae. |
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| Journal |
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| Reference |
3 |
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| Authors |
Tong, Y., McCourt, B.S., Guo, D., Mangla, A.T., Zhou, W.X., Jenkins, M.D., Zhou, W., Lopez, M. and Nes, W.D. |
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| Title |
, Stereochemical features of C-methylation on the path to Delta24(28)-methylene and Delta24(28)-ethylidene sterols: studies on the recombinant phytosterol methyl transferase from Arabidopsis thaliana. |
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| Journal |
Tetrahedron Lett 38:6115-6118 (1997) |
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| Reference |
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| Authors |
Bouvier-Nave P, Husselstein T, Benveniste P. |
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| Title |
Two families of sterol methyltransferases are involved in the first and the second methylation steps of plant sterol biosynthesis. |
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| Journal |
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| Sequence |
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| Reference |
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| Authors |
Nes WD, McCourt BS, Zhou WX, Ma J, Marshall JA, Peek LA, Brennan M. |
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| Title |
Overexpression, purification, and stereochemical studies of the recombinant (S)-adenosyl-L-methionine: delta 24(25)- to delta 24(28)-sterol methyl transferase enzyme from Saccharomyces cerevisiae. |
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| Journal |
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| Other DBs |
| ExplorEnz - The Enzyme Database: | 2.1.1.41 |
| ExPASy - ENZYME nomenclature database: | 2.1.1.41 |
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