KEGG   ORTHOLOGY: K09832
Entry
K09832                      KO                                     

Name
CYP710A
Definition
sterol 22-desaturase [EC:1.14.19.41]
Pathway
ko00100  Steroid biosynthesis
ko01100  Metabolic pathways
ko01110  Biosynthesis of secondary metabolites
Brite
KEGG Orthology (KO) [BR:ko00001]
 09100 Metabolism
  09103 Lipid metabolism
   00100 Steroid biosynthesis
    K09832  CYP710A; sterol 22-desaturase
 09180 Brite Hierarchies
  09181 Protein families: metabolism
   00199 Cytochrome P450
    K09832  CYP710A; sterol 22-desaturase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.19  With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
    1.14.19.41  sterol 22-desaturase
     K09832  CYP710A; sterol 22-desaturase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, plant type
  CYP710 family
   K09832  CYP710A; sterol 22-desaturase
Other DBs
RN: R07489 R11097
COG: COG2124
GO: 0000249
Genes
ATH: AT2G28850(CYP710A3) AT2G28860(CYP710A4) AT2G34490(CYP710A2) AT2G34500(CYP710A1)
ALY: 9317081 9317405 9317406
CRB: 17887986 17889424 17889482
CSAT: 104699232 104699233 104700630 104750004 104750006 104753438 104781842 104781843 104786110 104786111 104786814 104789238 104789239 104789240 104792202 104792203
EUS: EUTSA_v10016547mg EUTSA_v10016552mg
BRP: 103865343 103867449
BNA: 106396203 106432759 106432788 106447495
BOE: 106339100 106339917 106340751 106341405
RSZ: 108807244 108833237 108852806
THJ: 104814293
CPAP: 110807637
CIT: 102618952
TCC: 18596516
EGR: 104440796
VRA: 106776160
VAR: 108340983
VUN: 114188144
CCAJ: 109789198
CAM: 101489453
LJA: Lj6g3v1932820.1(Lj6g3v1932820.1)
ADU: 107468177
AIP: 107623676
FVE: 105350241
RCN: 112166774
PPER: 18778941
PMUM: 103325114
MDM: 103434989
ZJU: 107413371
CSV: 101221194
CMO: 103484375
MCHA: 111015778
JCU: 105650293
POP: 7472572
PEU: 105117943
JRE: 109021374
QSU: 112009839
VVI: 100255505
SLY: 100136886(SD1)
SPEN: 107011895
SOT: 102581211
CANN: 107859112
NTA: 107763057 107771426(SD1)
NSY: 104234654
NTO: 104096813
NAU: 109208410
INI: 109192033
LSV: 111890946
CCAV: 112504656
DCR: 108199974
BVG: 104883528
NNU: 104597733
DOSA: Os01t0210900-01(Os01g0210900) Os01t0211000-00(Os01g0211000) Os01t0211200-00(Os01g0211200) Os01t0211600-01(Os01g0211600)
BDI: 100827562
ATS: 109746138(LOC109746138) 109757150(LOC109757150) 109772439(LOC109772439)
SBI: 8079522
ZMA: 107305676
DCT: 110110071
PEQ: 110029296
AOF: 109828814
ATR: 18435199
CRE: CHLREDRAFT_196874(CYP710B1)
MNG: MNEG_5387
APRO: F751_0963
DDI: DDB_G0269016(CYP524A1)
DFA: DFA_11768(CYP524A1)
 » show all
Reference
  Authors
Morikawa T, Mizutani M, Aoki N, Watanabe B, Saga H, Saito S, Oikawa A, Suzuki H, Sakurai N, Shibata D, Wadano A, Sakata K, Ohta D.
  Title
Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato.
  Journal
Plant Cell 18:1008-22 (2006)
DOI:10.1105/tpc.105.036012
  Sequence

KEGG   ENZYME: 1.14.19.41
Entry
EC 1.14.19.41               Enzyme                                 

Name
sterol 22-desaturase;
ERG5 (gene name);
CYP710A (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
ergosta-5,7,24(28)-trien-3beta-ol,NADPH:oxygen oxidoreductase (22,23-dehydrogenating)
Reaction(IUBMB)
ergosta-5,7,24(28)-trien-3beta-ol + NADPH + H+ + O2 = ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADP+ + 2 H2O [RN:R07506]
Reaction(KEGG)
R07506;
(other) R07489 R11097
Substrate
ergosta-5,7,24(28)-trien-3beta-ol [CPD:C15778];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
ergosta-5,7,22,24(28)-tetraen-3beta-ol [CPD:C05440];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A heme-thiolate protein (P-450). The enzyme, found in yeast and plants, catalyses the introduction of a double bond between the C-22 and C-23 carbons of certain sterols. In yeast the enzyme acts on ergosta-5,7,24(28)-trien-3beta-ol, a step in the biosynthesis of ergosterol. The enzyme from the plant Arabidopsis thaliana acts on sitosterol and 24-epi-campesterol, producing stigmasterol and brassicasterol, respectively.
History
EC 1.14.19.41 created 2015
Pathway
ec00100  Steroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K09831  sterol 22-desaturase
K09832  sterol 22-desaturase
Genes
ATH: AT2G28850(CYP710A3) AT2G28860(CYP710A4) AT2G34490(CYP710A2) AT2G34500(CYP710A1)
ALY: 9317081 9317405 9317406
CRB: 17887986 17889424 17889482
CSAT: 104699232 104699233 104700630 104750004 104750006 104753438 104781842 104781843 104786110 104786111 104786814 104789238 104789239 104789240 104792202 104792203
EUS: EUTSA_v10016547mg EUTSA_v10016552mg
BRP: 103865343 103867449
BNA: 106396203 106432759 106432788 106447495
BOE: 106339100 106339917 106340751 106341405
RSZ: 108807244 108833237 108852806
THJ: 104814293
CPAP: 110807637
CIT: 102618952
TCC: 18596516
EGR: 104440796
VRA: 106776160
VAR: 108340983
VUN: 114188144
CCAJ: 109789198
CAM: 101489453
LJA: Lj6g3v1932820.1(Lj6g3v1932820.1)
ADU: 107468177
AIP: 107623676
FVE: 105350241
RCN: 112166774
PPER: 18778941
PMUM: 103325114
MDM: 103434989
ZJU: 107413371
CSV: 101221194
CMO: 103484375
MCHA: 111015778
JCU: 105650293
POP: 7472572
PEU: 105117943
JRE: 109021374
VVI: 100255505
SLY: 100136886(SD1)
SPEN: 107011895
SOT: 102581211
CANN: 107859112
NTA: 107763057 107771426(SD1)
NSY: 104234654
NTO: 104096813
NAU: 109208410
INI: 109192033
LSV: 111890946
CCAV: 112504656
DCR: 108199974
BVG: 104883528
NNU: 104597733
DOSA: Os01t0210900-01(Os01g0210900) Os01t0211000-00(Os01g0211000) Os01t0211200-00(Os01g0211200) Os01t0211600-01(Os01g0211600)
BDI: 100827562
ATS: 109746138(LOC109746138) 109757150(LOC109757150) 109772439(LOC109772439)
SBI: 8079522
ZMA: 107305676
DCT: 110110071
PEQ: 110029296
AOF: 109828814
ATR: 18435199
CRE: CHLREDRAFT_196874(CYP710B1)
MNG: MNEG_5387
APRO: F751_0963
SCE: YMR015C(ERG5)
KMX: KLMA_60417(cyp524A1)
NCS: NCAS_0A12700(NCAS0A12700)
NDI: NDAI_0B01560(NDAI0B01560)
TPF: TPHA_0I02420(TPHA0I02420)
TBL: TBLA_0H02650(TBLA0H02650)
TDL: TDEL_0B06510(TDEL0B06510)
KAF: KAFR_0A02110(KAFR0A02110)
PIC: PICST_73043(ERG5)
SPAA: SPAPADRAFT_63544(ERG5)
CAL: CAALFM_C702840CA(ERG5)
SLB: AWJ20_75(ERG5)
NCR: NCU05278
NTE: NEUTE1DRAFT116692(NEUTE1DRAFT_116692)
MGR: MGG_06047
SSCK: SPSK_00476
MAW: MAC_08636
MAJ: MAA_01109
CMT: CCM_02962
BFU: BCIN_07g01120(Bcerg5)
MBE: MBM_06688
ANI: AN4042.2
ANG: ANI_1_350014(An01g02810) ANI_1_446094(An11g03230)
PBL: PAAG_11073(PAAG_00230)
ABE: ARB_03814
TVE: TRV_05093
PTE: PTT_19871
ZTR: MYCGRDRAFT_55438(CYP-70)
SPO: SPAC19A8.04(erg5)
CNE: CNF03720
CNB: CNBF1100
TASA: A1Q1_00690
HIR: HETIRDRAFT_141009(cpm111)
LBC: LACBIDRAFT_187166(ERG5)
ABP: AGABI1DRAFT66440(AGABI1DRAFT_66440)
ABV: AGABI2DRAFT189837(AGABI2DRAFT_189837)
MGL: MGL_0310
MRT: MRET_0994
DDI: DDB_G0269016(CYP524A1)
DFA: DFA_11768(CYP524A1)
 » show all
Reference
1  [PMID:8543054]
  Authors
Kelly SL, Lamb DC, Corran AJ, Baldwin BC, Parks LW, Kelly DE
  Title
Purification and reconstitution of activity of Saccharomyces cerevisiae P450 61,  a sterol delta 22-desaturase.
  Journal
FEBS Lett 377:217-20 (1995)
DOI:10.1016/0014-5793(95)01342-3
Reference
2  [PMID:8635732]
  Authors
Skaggs BA, Alexander JF, Pierson CA, Schweitzer KS, Chun KT, Koegel C, Barbuch R, Bard M
  Title
Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol biosynthesis.
  Journal
Gene 169:105-9 (1996)
DOI:10.1016/0378-1119(95)00770-9
  Sequence
[sce:YMR015C]
Reference
3  [PMID:16531502]
  Authors
Morikawa T, Mizutani M, Aoki N, Watanabe B, Saga H, Saito S, Oikawa A, Suzuki H, Sakurai N, Shibata D, Wadano A, Sakata K, Ohta D
  Title
Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato.
  Journal
Plant Cell 18:1008-22 (2006)
DOI:10.1105/tpc.105.036012
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.41
IUBMB Enzyme Nomenclature: 1.14.19.41
ExPASy - ENZYME nomenclature database: 1.14.19.41
BRENDA, the Enzyme Database: 1.14.19.41

KEGG   REACTION: R07489
Entry
R07489                      Reaction                               

Name
beta-sitosterol,NADPH:oxygen oxidoreductase (22,23-dehydrogenating)
Definition
beta-Sitosterol + NADPH + H+ + Oxygen <=> Stigmasterol + NADP+ + 2 H2O
Equation
Reaction class
RC00001  C00005_C00006
RC01886  C01753_C05442
Enzyme
Pathway
rn00100  Steroid biosynthesis
rn01100  Metabolic pathways
rn01110  Biosynthesis of secondary metabolites
Orthology
K09832  sterol 22-desaturase [EC:1.14.19.41]
Other DBs
RHEA: 46579

DBGET integrated database retrieval system