KEGG   ENZYME: 1.14.14.102
Entry
EC 1.14.14.102              Enzyme                                 

Name
N-methylcoclaurine 3'-monooxygenase;
N-methylcoclaurine 3'-hydroxylase;
CYP80B1 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
(S)-N-methylcoclaurine,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (3'-hydroxylating)
Reaction(IUBMB)
(S)-N-methylcoclaurine + [reduced NADPH---hemoprotein reductase] + O2 = (S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R05732]
Reaction(KEGG)
R05732
Substrate
(S)-N-methylcoclaurine [CPD:C05176];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
(S)-3'-hydroxy-N-methylcoclaurine [CPD:C05202];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein involved in benzylisoquinoline alkaloid synthesis in higher plants.
History
EC 1.14.14.102 created 2001 as 1.14.13.71, transferred 2018 to EC 1.14.14.102
Pathway
ec00950  Isoquinoline alkaloid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K13385  N-methylcoclaurine 3'-monooxygenase
Genes
PSOM: 113314340
Reference
1  [PMID:9681018]
  Authors
Pauli HH, Kutchan TM
  Title
Molecular cloning and functional heterologous expression of two alleles encoding (S)-N-methylcoclaurine 3'-hydroxylase (CYP80B1), a new methyl jasmonate-inducible cytochrome P-450-dependent mono-oxygenase of benzylisoquinoline alkaloid biosynthesis.
  Journal
Plant J 13:793-801 (1998)
DOI:10.1046/j.1365-313X.1998.00085.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.102
IUBMB Enzyme Nomenclature: 1.14.14.102
ExPASy - ENZYME nomenclature database: 1.14.14.102
BRENDA, the Enzyme Database: 1.14.14.102
CAS: 202420-37-9

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